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CRIMIC CR02986

Gene CG10280

CG10280

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	3R:6011471..6011472	83D2-83D2	FBti0228260	97721

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CTTCGAGCATGAGTTCACCAGTGT	TTGGATGCCTCATTGGATTGACGC

Images

Construct Design Sequences

sgRNA sequence	ATCGATGTGATGTTATATTTTGG
sgRNA2 sequence

 

left_200bp	right_200bp
GAAGGTGCGTTCCACTCCCAGTTTCTCCCATGTCTTTATTAATCACTAAGTCCATTTTTGTTTGATTTGAGTTGTGAAGCCCTAAAGATCTTTTCTAGTTTAATCTCCGAACTAAAGAATCTTTTAGGAATATCGTATTTTCTCATTCTGTTCAGTGAATTCGCAATGTGAGTGTTAAAAGGACAATGCAATAACCAAAA	TATAACATCACATCGATACGTGGGCCTTAAAATTTCAAGAAAATACCATATGGCATATTATATATTCTAAATTAGTTTTGAAACCCCTTTCAGATGAATACGATCCCTACGGACCCGACATCGGTGACGATGACCTGGGAATCGACGATCCGGAGACCATGCCCCGCTTGTTCCAAGGCGACATCGCCATTGATCCGTAC

 

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