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CRIMIC CR02993

Gene Lsd-1

CG10374

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	3R:23765362..23765363	95B1-95B1	FBti0228262	97723

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCCCTGAATGATAACTTTTCGCCG	GAGAAGCGTTGACCATGATGGATC

Images

Construct Design Sequences

sgRNA sequence	GATAACCTTCCCGACAACGTGGG
sgRNA2 sequence

 

left_200bp	right_200bp
ATGTTTGGCCACAAAAGTTAGTATGCAAAATTACATATTTCTCCATATAACTAGAGATAATCAAAATCATATGAAAGATAGTTTAGTTTGCCAAAAGTTTTACATAAAAAGTAAGCGAAAGCAAGTGATTTGAAAAGTCAGCATCCAGGCAAATCACCTTAAGGTGCACTCATCTCATAGTTTATTTATAAATGCCCACG	TTGTCGGGAAGGTTATCTCTGGGGAACGGCGGATTCATTGAAACCTGAAGCCAGAGAAAGTTGCGATAAGCTGAGCTCTGGCCGGAATTTGCTCTCTCATGCTCAAAAAAAATTGTATAAGACACTTATTCCGAGTATCCGCCTTGCAGGCCCACAGGAGGATGATAACGAGGCCGTGCCGGACGAGAGGGGCGCCATCA

 

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