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CRIMIC CR02996

Gene Zip89B

CG6898

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	3R:16307967..16307968	89B12	FBti0228263	97724

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AACGGTGATTAGTACGATCGTCGC	GACCCTGTTTCCTCATGGTGAT

Images

Construct Design Sequences

sgRNA sequence	GTCATCTCGCAAAAATCAGGGGG
sgRNA2 sequence

 

left_200bp	right_200bp
TTTAACCGGAATCGATTCGGGGAAATTGGGAACAGGTTCTATATAGCTGATCTGCTAAATGCTGCGTGGTTGACCTTGTAACAGACCTTGCTGTGATTTATTTTGCATACTCATATACAAGACGGCTTGTCAGTCTGTGTACCTAGAGTAAATGTGAAATAAAAATCCCGTCCCTGCCATGAAGTCATCTCGCAAAAATC	AGGGGGTTGCCCATGCGAAAAAGCACCGTGAAAACCGCACTCGCAATTGTATTGATGAGCACGGGATAAAAAATCAGACGAGAAGTTTAGGCCACAAATATGTGATTACCCGAAAGTCGGTTAGGTTTTTATATGGTCTAGGGCCAGATAAGGCAGGACAAAACGCTCATAAATCATTGCGTGGAAGTGGGCTGTTGGAA

 

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