CR60004

CRIMIC CR60004

Gene CG7332

CG7332

Information

Donor Location Cytology FBti Stock number
pM37_p1 X:18884513..1888886017F2Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK OK

Images

Construct Design Sequences

sgRNA sequence AATATGAATGAACATGACCTGGG
sgRNA2 sequence
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
 
Amplicon 5' Amplicon 3'
GGATTGCTTTGTTTAAATGTTACAACCAGTTTGACTTGTAATATTTGTAATAACTTCCATAAGTAATCGCTTGTTTTGTCAAATAACACTTAGAAAAAGAGAAGTTTAAAAGGTAAAGCTTAAAATTAAACAATCTCTAGACCTAGAAATTAGTAAGCTTTGCTTGCTAGGGTTCGTATTTGCAGACCGCCAATCGATTGTTTACGTATTTGCGCACACTCTGGCCACATTGCCCCACACTGTTGCACTAGTCAATCGATTGGCTGTGCCAAATAACGCCTCCTGCTATCGATATCAATCAAGAAAAAATAAAATTTATTAAATAGATTTGTACATAGGAAATCGGGTGACGCGAAGCGAATAACATCTGGTCTAGCCAAAAATGCCAACCAAGTTGGTGGGCTAAAAGAGTGAATCCGAAATCGAGGACTAGGAGCAGCAGAAGGAGTAGGAGCTGACCACCAGTGGAGGAGCGACCGTGCGAACGAGCGAGCAAACGGAGCGGAGCAGAGCAGTAAAAGCAGTGCAGCCGTTGATTTCATCGGAGATGAACGAGAAAATCGTGCGGGAGCAGCGCGGCGGCGAAGATTCCCCATCCTCCGTCTCGGCGAAAAGTGCAACGGCAGCGGCGTCAGCGTCGACAGCGTCGATGACGTTTGCCTCGGCGGCCTTGGAATCGCACAAGACGACCACCATCACGACCGCCAGCAGCAGCCCTCGCACCAGTGGAGCAAGTGCATCCGCCTCGTCATCCTCTCGATCCGCATCTGCACCTGCATCGTCGTCCAGCCAGCAGGAAAAGCAACCGATGCTGAATGCCACCGATATGCGCGAACTGCGCGAAATCAAGCAGTTGCTGTGGGGCGACAATGTGCGCGAAGATGTATTCAAGCGTTGGTCTCAAGGTAACCACCCATCTCCTCATCTTCCCATACCCCATGCACATAGTATGCACCACCACCCATTGTGGGGCAAAAAGCGCGCCCTTTTTGCGCTCGGCAAGGCTGCGTCCAGTTGGAATGGGCGCTTTTATCTTCAACTTGGCCCAGG CCAACTACAATCAGTTGCCTTTTCGCTAAGAATAGAAACTTATCCCAGATCATGTTTAAAGCATTGCTGTCAAGAAGCAAACATGAAGATTGTAAATACTCCGCGTCTGTATTTCCATTTTCATATCTCAATTCTTATAATTGGGCATTAATTGATTACTTCTTTTAGTGGCACGAGTAGTTGTAGTATTTAATGACTAACTGAGATTATAGACAAAGTTAATTAGTTAACCTGTCATCAAATTTTGAGATAAATAAGTCCCATTTCAATGCTCTGTGAGTATTGAGTATTATCATTCAAGTACCATTCCTTGTTATCAAAGCTTTTTTTTTGCTGCTATTCCCAGTTATTAGATATGAATATAAGATTTGGTAGCTTTATGAAGGCTGGTAAGGAAGGCTGTCCTCTTGTACTCCTTCTCCACTTTTCACATTGCAGTACCCATCTTCTAATTTTACTTACACTTATTTCTCCACCAGGATTCGAGTTTAGTAAAGTGGAACCGTCTGCATTGGTGCAGAAGCAGGGCGGTCCTTGTGCTGTGATAGCGCCGGTGCAGGCGTACTTGCTTAAGATTATTATCATGGACTTGCCAGGCATCAAGCTCTCGGAGGTTGGTCAACTTCTACCACTTGAGTACCGTACCGCGCTAATCTCCACATTTATTCAGATTTCCCTCGACAAATCGCAGAACCTGCTGATCCAGGCATTGTGTGACATCCTGAAGAATTGTCGGGCGCCGCGGTACCGCATCGTCCATCTCTTGCGCCGACGAGGAAACGCTACAGAGGCTGGATCAACCAAAAAGCGCTCACCGGCTGGCGAGGAAGAATCTGCTTTAGCTGGCCAAGCGGCAGGATCTTCCGAAGAGGTGGAGGAGGCGGCAGAAGCCACTCCCGCCTCGGTCAGCAAGTTGTCCCAGGCCCTGCAGCTCGAACACGATATGCACCAGGAACTGTCGCCGGATGAGTTCCACGAGCGCCTGCACACGCTCCACTTTAAGAATATCGCAGCCGTGGCCCGCTACTATATGGAGAATTACGACCAGCTGGCGCACACATACGGCGTGCTG
 
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