CR60018

CRIMIC CR60018

Gene Nplp1

CG3441

Information

Donor Location Cytology FBti Stock number
pM37_p0 2R:25032377..2504037760F1Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK OK

Images

Construct Design Sequences

sgRNA sequence TTATGTGTTGACGTGTGCGGTGG
sgRNA2 sequence
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
 
Amplicon 5' Amplicon 3'
CGCTGAGAGCGCCAACATATGGCGTACAGCTCAAGTTCGAATTAAGAGAAACCAAAAACTCTTAGATTGGTCCCAAAGCAACCAGCGCACAGTGGATTGAACTTGCAAAGTTGAAAGAAAAATGATAAGAAAACGGTTTCGAGTAATTCAAATTATACCGAAACCCGAAAAATCGTAGCTAAGGAAGGGTTTTCTATTAATTTTTTTATTTTGGACTTGGATTCATTTTAATTTTTTTTTGGCCTGCAAACATTCTATTATATTTTCAAAATGAATCAATAATGTAACCTGCGAATACCAATTTAATATCGAAAAGCTTTGTAGCTGTGCACTGTGCTGCTGGTTGGCCTGAATACACAGAGCTTACCAAGAAGCAACAGAGCTCTGTCCTGCAGCCGCCATGACGTCATTACCTACAATAGCATCCCACGAAACAGGACACTGCACACGCAACAGAGAGGCTATAGAAGAGCAACAGTTGACGGCGCTCCGGTTTCGCTCCCGCGAGTGAGCTTATCCGGGGTGCCGCTCGGGTATATAAAGTCGAGGGTCCTGGCCAAGAGAAAGCAGTCGAGCAGACGACTTTGCGGCTAACGTTAGTGTTACGGCAGCTGGCAAACGGCAAAGCTTCGCGTCCGTGTTTCCGTTACGGTTTAAATCGGTTTGGAAGTGGGTGCTGCAAAAGGGCAGTTCAATTTGTGAGGCAGTGCGAGTGTGGTCGATCCTTTTGTCGCGTTGCAAACGAACGAAACTCACTTAACAGTTGAGCCGCCCCCAGGACGACATCCACGATGCAGGCGGTGCTCCAATCTGCGCACAGCAGCAGGCGGCTAATGCTGTTGCTCTCCATGCTGCTCAATGCCGTAAGTCCTGGGGATTATATTTTCAATTTTAAAAACTGTAGCGCTATCAACATTTTTCGGTCTTTGGGTTTCGTGGACTGTGCTTCTGCCTTCTAC CGGTGGGCCGTGAATTTTGTCGAAATTCGTTTGAGGACTGCGTGTTTTCTCGACGAACGAAAATTGAAAACGCGATATTATCCTTAGAAATCTTTCATATTTATAAAAGCTAATTAAAGAGCTGACATGCTTCTTCTTCTTCTTGCATTTCTAACCCAAAGTTTAGCATGATTACATCTCACCATTGTTCAACGAAGTCTCCGCTTTTCATAACTTGTGCTGTCAGAATACGCAAACTTGTCTACAGCTTCTAAGCTCCAGTGGCTACAAAACGAACAAATTATTTCCATTTTTCAAGGACCCCGTTCACCACGCAGTAATTTATATTGGTACAGCCACGCCCATTCAGGCATAACTTTCCGCGGGTTGTTTTACTTTTGCAAGAGATACATCTGCGGTTGCAACCTCATCCTTCTGGTCAGAACGAGCACAACACGTTTACAGTGCAGTTATTCATATGGCATTCTCACTTTTCCTTTTGGTACATTCAACCATCTCTTGCGGAATAGCTGGCATTCCTTTTGTCCCTCACTGACATATGTATGTGACATTTCTTCATTTTTCATTTCCGCGGAACTTGGCATTTCCCAGAGAGCCTTTTATGATAACTGGCTGCAGGAAAGCCACCTTTAACTACATACCTAATCAAATTGTTTTTCTAGCTATAACAAAGAAAGAACTTTCATGTCGAGCATCTCTCCTCTGTTGTCTTTTACCAAACACAAATAAAATTACATAAAATATTATTGTTGACAGTTTTTGCACGGAAAAAGCCACGGGCTATGAAAGGCTTTGACGGTCAGGAGCGGAACTTTTCATTTTAGATAAAGTTAACAGTTTAGTTCCTCAAATACTTAAATATCCTTACGTCAGTAAATCCAGCAACGTCAGAGGAAACTGCAACGTCAATGACAACTATTGTACAACATGGTTCAATGTAAACTTGCTTATGATTTCGAAGTTGCATCGCTG
 
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