CR60022

CRIMIC CR60022

Gene CG10543

CG10543

Information

Donor Location Cytology FBti Stock number
pM37_p0 2R:20996220..2100774057B5Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK OK

Images

Construct Design Sequences

sgRNA sequence TGTAATTTATAAGTCTTGAATGG
sgRNA2 sequence
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
 
Amplicon 5' Amplicon 3'
GGTTGGCCAGTCGAAGTGCGCATACACTGTCATTAGTGTATAGAAAATGGTAGTGTATAGTTGTAGTAAATGGTTCTATTCAAAACGAATTTATGAACATATTTATTGATTTATTTTATATTAACGTCGTATACTTTATAGACTTTTGGAATTATGTTTCGAAATAAATAAATCTGTGTGACGGTAGGGTATTTGAGGGCGTTTAAAACTTTTCAGGGTTGCGTCGTCGTTTACTTTTAGTCCCCTTCTACTCCACCCCATTTTGCCACCCACCCACCTCGGCTCCTCTCCACTGTTGTCCTCTCCCTCTCGCACAGTGGGTGGAGGAGAGCGGGCAGAGAGCAGCCCCACATTGTGTTTGTAACAACTACAAATCGCTGCAAATACACAGGCATACCAACGGCGAACATACATACATAATAGCAACATTGTGGAATGGGGAAAAGTAGCACAAATTGTGCGCTGTTTTAATTTCTCTGCTTCTTCTTCTGCCTCCGCTGTTGCTGTTTTTGTTTTTCCTTTTCTTTTCTATTTTTCGCCATCTTCGCTCGCTCTTGTGTCGCTGTGTTAGTGCTAATCGCTTGCGCGGACAGCGCAGTCGGCGTCGCCGTGCTGTCTACGCGCGCTCTCTCCTACTTCACTTCCCGCCCCTTCTCTATCTTCCACTCTAATTGCATTAAATTGTTTGGAAAATAATAATCAGTAATTGCGTTTGACGACAGTTATTTCCATCAGCGCTTTGCGAGAGAATTTTGCCGTTTTATTGAAACAAATGAAGATTCTAATTGGGTCTTTAGCGGAACTAACAAATCTCTCTCACTAAGGTGGCAACCCTGCCGCAAACCCCGTCCCTCCCACCGACCACATTCCGTACTTTGTTGTTTTCTCCGTGGAAATGGAAATGGGCGGCTTGAAGCGGAAGTTGGTAATTTATTTTCCACTAGCGCAATAATTTAGGCACCGTGGGCTTAAAGCGGCCATTC GCATACCCACTTTTAGTTCTTGATTAGCTAAGCGCATTTCTATGTCACCTATTACTTTCCAGGTGGATCAATCACTAAATCTACTGGACAGCCTCAACACGGCATTGCGGTGCGATGCTATTCAATTCTTGCTGCAGACGCTTAAGTGCAAATACCCGGAGTCCTGCGACCAAGTCGTCAGGAATCTGTTCAGTCACATAGCTGCGGCCAATGACAATGGCGAGTCTGGAGGCAGGGCGCAGCAGCACGAGCTAGCGAGGACGAAGCAGCTCCAGCTGCTGCTGACCGCCAAAAAGGAGAAGAAAGAGCTGGGAATCGGTTGCGAGGCGGAGATCAAGCGAGAGAACGATGAGGAGGAGGCGGGCTCGACGGATCTGAACCAAAATTTCGAGAAGATACCCTGCAAAGAGGAGTTCCTGTGCCTCGAGGAGGAAGAGGAGGACTTTCTCGACGATGCGGACACGCAGAACTCGTCTTCCCTGCAGTTCCACACCGGTAACAATGTGGACGCCCTGGCCCTGGGCCCTGGCGATCCGCTGGAGCTGATCCAGTCCGGAGTTTCGCTGCTGGTCAAGCGGAAGTATGCTGCCACCTTCGATGACGAACTGATCGGGGATGGGGATGGTGATGAGGCCAACAGTAACAGCAGTGACGGCAAGATGGTCAAGCGAAAGCGCACCAACAACATGCACCTGACCGTGTCGAGTGTGCGACGGAAAGAGGAGCACGGCCAGCTGGGCGATGGCTATGTCTTCCACGAGGACAGCCAGTATACGATGCGATATCACCACAGCACCGGCGAGTTCAGTGAGCGGGCCTTTAAGGCCCAGTTCCGGGCTCTGCTCCGCCTGGCGCTCAGTCAGCACCACAAGCCCTTTCTCCGCCAGTTCTACGAGAACTTTGTTGTGAATGGACGCTTGCTGGGGACCACGCCCTCGATGCGTGTCCTGAAGGAGCTGCGCGAACAGCGGCTGGAGGAGTGGCGACGTCAGCGTGAACGCCGCCAACTGGTCATCCTGATGGAGCAGAG
 
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