CR60032

CRIMIC CR60032

Gene CG5726

CG5726

Information

Donor Location Cytology FBti Stock number
pM37_p2 2R:18175255..1818019755B12Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK OK

Images

Construct Design Sequences

sgRNA sequence GCTGCTATGCGTTATATCAGTGG
sgRNA2 sequence
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
 
Amplicon 5' Amplicon 3'
GGTGGTGAGGGAACGGGAGCGCGCCTCCGGGATGTTTGTACCATCCTGAACACTGGCCCCGCCGCCTATCAAAATCAAACAGGTGGCTATGCCTACGACCACATCATCGCCCTGATGAGGAACCTTGATCTGCAGGACGATGGCATTGTGCTGAACTCCAAGATCAAGACCATCGAAACGGACTTCTGCAACATAGTGCGCGACGAGTCTATGCTTTGGTATGTGTGCCGATCTCTACCGTTTGGTTGGTTCGCTCATTGATGATTAATCATATCATAAGTAGTGTGTTTATTTGCCACCTCTTTTCGCATATACATATGGATGTATGTAAATACCCGGCGTAGGTACAGTGCGGTGCGATAGCATATGGGCAAGGCGAGCTCCCAAGCCCATCAAAGCCAGCCATGTGATAACAAGACTTAATGCATGCAGTACATATCTGCCTGGCGAAGACCGATCCTTATTGTAATATTTATGACATTCCATTACAAGTAGCATGAGTAATTCGTTAAATTTGGGAGCTTGTGGCGTTCGCCCATAGGTTACAGCATTCTATAAGTTATTCCAATAATGAAATGCTTGAAATGCAGAAACCGAATTACTAATCAAGTTCAAACGTTAATTTGTTTTTACGCGTTCAAGAACCCTTGGGTTCTTAAAGTGCAACTTTGTAAACTTTTCTTCTTACTTCATTATTTTTAGGTTCGAAAAAGTTTTTGCGACCTTCTACATATACATTTCAATTTTATTAAATGTCTAAAAATCGATTTATAAATCTGAAAAGCACTGTATTTGTGTATATGTGTTCGTGCACGAGTTCGTTGTTTGCGACCAGGTTGCTCGAACGTCAGCGTGTCTTGAAAATTTACAACTTTTCATACAAATAAAGACGGGCAGAGAGACATCGGCGCCATCGAATCATTTCAAAGTGAACATTTTTCCCTCCGATTCTCTCAGGCTCATGCTCGAGTTAATTGCTGAACAAATAGTTCGCAGCTTGGGCTTATCAAACAATGCTGCTATGCG CAGTGGGGGTGTTAAACACAGTTGTAGTGCAAACTTTAACATTATGAAGTAGTAGTAGACTTGATTATTATAACATACTAATAAATGAAAAGTAAATAAACTAATTGTATTTGCAATTTTCAGTGAGTCAATGGAGTACCTGAACGACAAGGCGCTTAGCGATGGTGATACGGCGCTAAAGTTCGCCCTGCTCTTCTCATCGAGAAATTTCGACGCACTGGCCATGAAAGAGACGAAGGTGCGCAGCGCCATGCTCAAGATTTTGGAGACAAACTTCCTCAACGCGGACGCATATCGGTTGCACGACAAGAACCGACTGTACAACTCGATCACGCTGCTGGGTGAATATTATCACAGGGTAAGGCTGGCGGATAATACCCCGATCACCATTTTGGGTGAGTCCCTGCTGGACTTGCTTACCCGCGAGCTCAATGACACGTCGGTGGTGCTGGGCACTCGGATTGCCCGCCTGGTGCTGTCACAGATCACACTGAATGGGGAGATCATGCGCAAGCGGCATAAGGTCGAGATTGATTTACTGCTCTTCCACGTTCGCCGGCATTTGATCATGCAGCCCGCGCTGACCGCTAAGGTGAAGGCCATGCTACTGATGGTGCTGGACCTCTTTTACAGCCACTTTAAGCATATCGGCCACGACCTGGAGACAATGTACACCAACTTCTTGGTGGTGGAAGATGGCGAAGAGGATGGGGTCAACAATAATGGCTCCGGCCCCCAGGATCTCGCCGAAGATGGCCGCTCACAGCAGCTTCAGCATCAACAGTCAGAGAACGGCAGCAGCGCCAACACGTCCGGCCAGGATCAGGATACCTTTGAACCGCGGCCGTCGGCAAAGAAGTGGAGCGAGCAGGTGTGCGAGGATTCCTTTTGTGAAACTGGCTACGGTGAGGCGGATCAGGGCGACGATAGATACTCCGAATCTGGACTCTCGTATCAAGCGAATAACTCTGCTTCACTCGGCCACCGTCGTCGCGGATTCCAACCTCGGCAAGTG
 
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