CR60056

CRIMIC CR60056

Gene Madm

CG1098

Information

Donor Location Cytology FBti Stock number
pM37_p0 3R :..Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK OK

Images

Construct Design Sequences

sgRNA sequence AAACTAAATCTCTCACTCGATGG
sgRNA2 sequence
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
 
Amplicon 5' Amplicon 3'
GGGCGTCAATCTGGACTCGTCGCCACGCGAGTCCGGGGACGATTCGGAGGATGAGAGCGAGATTTTGGAGGAGTCGCCATGTGGACGCTGGCTGAAGCGACGCGAGGAGGTGGATCAGCGTGACGTACCCGGTATTGACTGCGTCCATCTGGCCATGGACACCGAGGAGGGCGTAGAGGTCGTGTGGAATGAGGTGCAATATGCCAGCCTACAGGAGCTGAAGTCGCAGGAGGAGAAGATGCGGCAGGTGTTCGACAATCTGCTGCAGCTGGACCACCAAAACATTGTCAAGTTCCACCGCTACTGGACGGACACGCAGCAGGCCGAGCGACCCAGGGTGGTCTTTATCACCGAGTACATGTCGAGCGGATCGCTGAAACAGTTCCTCAAGCGCACCAAGCGCAACGCCAAGCGGCTACCGCTGGAGTCGTGGCGCCGGTGGTGCACTCAGATTTTGTCCGCACTCAGCTATTTGCATTCCTGCTCGCCGCCCATCATCCACGGCAATTTAACCTGTGATAGCATCTTCATCCAGCACAACGGCCTGGTCAAGATCGGCTCGGTGGTCCCGGACGCGGTCCACTACAGCGTCCGGCGCGGCCGAGAGCGGGAGCGTGAACGCGAACGAGGCGCCCACTACTTCCAGGCGCCCGAGTACGGCGCCGCCGACCAGTTGACCGCCGCCCTCGACATCTACGCCTTCGGGATGTGTGCCCTGGAGATGGCCGCCCTGGAAATCCAGCCCAGTAACAGCGAGTCAACTGCCATCAACGAGGAGACCATCCAGCGCACAATCTTTAGCCTGGAAAACGATCTGCAGCGCGACCTGATACGAAAGTGTCTCAACCCGCAGCCGCAGGACCGACCCAGCGCCAATGATCTGCTCTTTCACCCATTGCTTTTCGAGGTGAGTTTTCAAGTCAATTATCTTGTTCTAAATGCTATAAATTCAAGACCCGCATAACAATTGCCTTGTCAACTAGACCTTGCATTCTAATTCTCGGATCCATCG GAAATCTGAAGATTTGAATAATTATATGATTTAAATGCACCGTTAAATATTTAAGTTCTAAATTTGGTTTTATCCGAATCATATCGTAAATTATCTTAGAATTTAATATATTATATTCATGTGTATATTATATATATATGTATATAAATGGAAGGAGAACTAGTAATCAGTTGATAATTCTACAATCCATTTTGACTGTAGTATATTATTTATTGCTCACAGTCACTTATATCTTAAGTTCAAGTTGTGGTTAATATGTAACTATACATTTTATTTTGATTACTGAGGGTCTGTTGTTTTATTTTCATACCTGTTACTATATTCGTTAAGAGGAAATTAACGGGTAGAAGGAATCGTTTTCAGCCCTATATTGTTAGCGATTCTTCATTAGAATCGTCCACTCCAACGGAAACCCCATAGAACTGCCACGCCGATACTTTGGTCTTATTTGTCTTGTCAATATCCATTGGTATGCCATTTTAAAGTGTGGAAACTTCTCGTTTGCACTTCAACTTGAGTCTGATATTCGAGAAAAACAGTGTAGCGTTCGCTCTTGTTGACTTTAAAATATAAATTTGTACATGGTTCAAATGACATTGCAAGGTATTCTTATAACACTTTTATAAAGGTAATTAACTGAAAAACTCAGTAGTTAATAATCAATTTTTTGCTGCTATTACTACAGGTGCACTCCCTCAAACTGCTGACTGCCCATTGCCTGGTCTTCTCGCCCGCCAACCGCACTATGTTCTCGGAAACCGCCTTCGACGGCCTCATGCAGCGATACTACCAGCCGGACGTGGTTATGGCCCAGCTGCGGCTGGCCGGCGGCCAGGAGCGGCAGTACCGACTCGCAGACGTTTCAGGCGCCGATAAGCTGGAAAAGTTCGTCGAGGACGTGAAGTATGGTGTGTATCCGCTAATTACGTACAGCGGCAAGAAGCCGCCCAACTTCCGTTCCCGGGCCGCCTCCCCGGAACGGGCTGACTCCGTAAAATCGGCCACGCCGGAGCCTGTGGACACGGAGTCGCGGCGCATCGTTAACATG
 
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