• About
• Search
• MiMIC
  • Technique
  • Constructs
  • RMCE Lines
• Transposons
  • GDP
  • Others
• CRIMIC
  • Technique
  • Constructs
• Order
• Downloads

CRIMIC CR60110

Gene Tbc1d8-9

CG7324

Information

Donor	Location	Cytology	FBti	Stock number
pM37_p2	3L :..		FBti0219181	Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK

Images

Construct Design Sequences

sgRNA sequence	GGAGCCGCCGATCACGTATTTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right

 

Amplicon 5'

Amplicon 3'

GAGTCCAAAGTCCTTGCGCGCGGCCAGAATTCGCTCTCGCATCTCGGCATGGGTCTTTAGCTCCGCTGGAGTTCGTTGTGGCACGATGCACATGGTTAAATCGTCGTAGAGGTCACATTTCTTTTTATAGGGCTTTGTGAAGCGGGATTCCTCCGCCTTGTGGTGGATGCGACGCAGCTCATCCTCGATGGTTAGCACTTGGGAGATCGTGGAGTTGGGTGGCTTTTTCTGACAGACGCGATCGCGGGGCGGACAGTTCTCCGCCTTCCATTCCGGCGACGTTTCGTTGCCCAGAAAGGTACGAATCTCATAGGGATTGTTAGAGGGACTGCACTCGGCGTTCACTTCCACGAATTTTTTCAACATTTTTGCAAAACACTGACGATTTTGCAGGAGTTGAGAATAAAACTAGTAATTTTGTAGCACGACACGTTCGTTGGGCTTTGAACTTGTTATATAAATGAGAGGTTTCAGTTTCGATTTAAAGCTAAAGGCTAACTATGTAATTTATATGGTAACTTTGCGTAATATTAAACTGTTAATAATGAACATTTTCTGTTTCTCTATTCGGCTTCTAAAAGCCAACAAGTTTTTAAATTGGAGAGAACATATTTCGCTTAATTGAAGTTAAGATACAGATTCATATTCTTAGGTTTAACTAAACGTTAACTATTTGATTCAAAAATCGGACGCAGAGTGCACCCATTCACAATAGATTGCTTAGTTCTTTGTGCGTTTTGTGCTTTCCAGCCAACGGGCACACTTGCCACAAAAACAGCAATCAGCGCTCGATGCAGAATTAAACAAAGAGTAATTTTGTATTTAGAGCCTGGCACGGGCTAAGAACAATCAAAATGTGGATTCAGCCCAAGGAGCTGCTGCTTCCCTCGGCATTTTGGTGAGTTATAGCCCAAGTCCGCAGCCGAATCGAAGGATTGCCGAAAATCCAAATTAAACACGGCTGCATGGGTGTGTGGGTGAATTGGAATGGACGGCATGTGTATATGCAATGTTATGTTTATGGAAGACATCTCATGGAATGCGATTATCGCGAAGTGCAGGCGGAATGGGGAGCCGCCGATCACGT

ATTTGGATTAGGCACAAACGTCGTACTTGCAGCTAGCTAGTTGAAATGCATTTGTTGATAACTAATTGAGCCAAAAGTTCAAGACTCCAACTCTTCAGATCGCTGGTTCTGATTTCGAAACGCAAAAAGTGCCAGCTGTGTAAAGTCAGCTTGAAAATTGATTTCCAACTGTTTCCTGCTGCGTAGAAAAGTGCAGACACCGCCCTCTAGAAAATAGCCCCCGTTAAGTGGGGCCCTCCCCCCTTGATAAGCCGACTCTAAAGGTGAACACCATAATTTTCGCCCACAGGATTGCCGAGATGCACTCGAGATACTTTGTCCTGCAGAAGAGACGTGGCCATGGGGAATCACGCGGATTCGGTTCCATGTTGGTGGGCACCTACGACAGCGTGATGAACACCAAACCGGCACCCTATCGCATCCTCCACCAGACGCCCTCGTCGGAGGTGTCTTACGGTAGGTCCAATATCTCAGACTCATCCGCTCTATCAGTTCCAGTACCTCGGGTCTATTTCGTATGTCCAGCAATCGGTGACCGTGAATCATCGGGCTTTGACTCGCATTTATGGGAGTATGCCAATATTTCTTTCATCTACGGTTCCCTCGGCGCTTACGTAAACATGGAACGGAGCTGATTACAAAAGCTGCGGAGGCTGGATCGTTCCTTTTGTTATTCAATTTATATTATATTAAGAAAAGAAGGCAACTGACGGAGTAGGGTACTTGAAAACCAGGCATTTTCATCTCAACATGCGCAAGGCGCTGAAATGCGTGTTATCCTAAAGTTTAGTAAAAAATGTTTAAACTAATTTTTGGTATCTTACTGTGCCTGTAGAATAATTAATTCCATATCCATATTGTGCTGATATGGTTTTTAAGTCGTGTTCTATAGATAAAAATATAAAAAAGATTAACAAGAATCTATTCCCTACAGAAATCGCCATTGGCATTACCCAGGATGAGATTGTTAAGGATTGGGAATGGCTGAAAGCTAATCTCTTTAAAGTGCTGGACGAGATGGAGAACGAGGACGAGGTGACCAACTTTAC

 

---

Search Again