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CRIMIC CR60112

Gene Rab11

CG5771

Information

Donor	Location	Cytology	FBti	Stock number
pM37_p1	3R :..		FBti0219182	Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK

Images

Construct Design Sequences

sgRNA sequence	TATCGGGTAGCATATCAACTTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right

 

Amplicon 5'

Amplicon 3'

TTGTTCCCTTCGTTTGCTACTACGCCATAAATTCCAATTGCGCTTGGAAATCTCTGCTAGCTATCACAGCTGAGCAACTGTTGAATGTCATTAAAAAAATATTTAAAACTTAATCTGCCGTATTTTTTGGGTCAAGTATACAAAAAGTTAAAAAAAAAGTCATCATAATAATGCGTCAGTGTGCGGTATGCATGATGAGGCCTCAAATTTCACACATGAAACAATATTAAACGGACATGACTCACAGCCAATAACTGCTGAAGAATTCCACTCACCGATAAGCGCATAATCACATAACTGAAAATGGGCCGTTTCGGGTTATTCGAGCCAATCCCACTACTAAGTAATGACCGTCGATTACCACTCCTCCCCTTTTAATTTCTCATCGCATCCGCTTAACTAAGTGCAATTTCTCTTTAAAGATCGTTATTAGCGCTGAGAAAGAGAGTAGAAAACAAAGTGACTCAGCTCTCTCGCTGATAACTGTAACCACGCTGCTCCCAGCTGTCCACACTGAAAGAAAACCCTATACCCTCTAATTTAGCTATCTAAATTAAGTCAAAAACTTGGACTTAGCTCAAAAGTCGTTTCAAATATTTAGTTCCTATCTTAATGGCATTGCAATCGAAACTTCGAACTCAAGAATCAGTGTTCATATGATACCATTTTACTATTTGAATTTTCTCCGTGCAGCGATGACCATAGCGATAGAGCGAAAAGGTGTTTGATGAGTTATAGTTGTTGTTTTCTGAATGGATTAGCAAACATGCTGAATCAACAATGTCCGAAAAGGGTGTCAAAAATGGAAACTAATAGACAGACAGTCAGTAAACACAGTGTCTAGATATCAGTTAATATATATTATAGCCAACAACTCTGGCCGCACATAAGAAAGTTTATCAAAAAGAAATGGAGTCGTAAAAAGGTCAGTGGATAGTTTTTATCGGGTAGCATATCA

ACTTGGGATGCTTGTTTGCTCCCTCAAATATCCGCCACTCAATTATCCAAGAAATGTTTGCTATCGGCAATGAAGCACAATAACACGAACTCCCTAACGTCGACTGGCCCAATCATAAAACATTTCTTTCCATGGCCTGAAATCCCCCGAGCATGGCGGTTTTATGTACTCGCTGACCTTGGTGCGCCGTGCCAGCGGCGAAAGGAGGACATGACCTAGCCGGGCCACCAGCGACCATTCTGTCCGGCCGTAAAACACAAGACAGGGGCCATGCCACGCCACGCCACACCACACACCCACATAAATTATGCAACTGGGGGTCTTCGATTATCCAATGCCAATGTCGATGTCACTAATTCCCTCTAATCCTTTGCAGTTGTCCTTATCGGTGACTCCGGTGTTGGCAAAAGTAATTTGCTCTCACGTTTCACGCGCAATGAATTCAACTTGGAGTCCAAGTCGACGATTGGCGTTGAGTTTGCAACGCGCAGCATAGAGGTGAGTACGCTGGCCAAGAATTCGAGGGTGGAGTGGCCCATGGAGCTCCACCCCCACACATCTACCGCACCGCACCCACAGTGGGGGAAGTCACGGGTGAACTTGCGTGTGCGTTTCGGTGGGAGGACACGTAGTTGAGGCGTCTATCGGAAAATTGGGGGGAAAAGTTTGTTCAATTTGAGTAAGTGTCGAGAATTTCACTAAATAATGGATTGCCAATTGGCTTTCTTGCATAATTTCAAAAGCCATCCTACGGGATTAGCCAAAATGTTGACATCATAGTTGTCACTTGTGCTGAAATAATAAATTTATGTGGTCAATAACAATTATATATTACGTATGTATAAGGATTTGTGTTAAACAATACTTTGTGCCATCTTTTCGAAGTAGCTTAGTAGGTCATCTTAATATTTAGTATATCAAATGGGTTCAATGAATATAACTTACGCTACTTGCGTTTTTTCTAACTAGTGTAAGAAATTTCAAAAATTGGGTTCTCTTACTAGAGTTAAAAGCTTATCCATAAGCTCTAATTTATGTTGGTTATGTGTGAATACTGAATGCCAGTG

 

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