CR60180

CRIMIC CR60180

Gene tow

CG14821

Information

Donor Location Cytology FBti Stock number
pM37_p1 3L :..Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK OK

Images

Construct Design Sequences

sgRNA sequence AAACTTCTCTGCGAAACGGGGGG
sgRNA2 sequence
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
 
Amplicon 5' Amplicon 3'
CAACTTTGTGTATGCCCGATCATTTGGAATGATCTATCAAATCGTTTAGGCATTTTCCTCTGTGTAGAGCAAGGGAAATTGGGGAAACGGCGAGGAAAACGTATTGCCTCGCACAACAAAAGCTTACACGCATGACGACATCAATTTGCCTCCAGCTGTGTGTGCATGTGTGCGTCCGTGTTTCCGTGCATGTGTGTGTGTGTGAGCCACCTGTTTCCACACAATGAGGACATCATTGATTAATATGTCACCAGCAGTGAAAAAAGAAGGGGAAGTTGAAAGGAGGAGCCCAGTTTTAAGGCCATACATTCACCAAAGTAATAATAAAGGCCTACATTTTAAAGCCCACAGCATTGTCTATATATAAACACACACACACGCACACTAGCCAATTTAAGTTTATAAGAATGTAAAAACCTAGCCTACATGGCAAACTTTTGACTATGCCCAACCCCGCGCGTTTGTTGCTGTCTGTGTCTATGTGTGCGTGTTTCTTGCCTTTTTTATTTCATTTTGTGTGTGTATAATTAACTGCTAAAAAGGCAACTTGTTGTTGCAGTGGGCGCCGCTTCAAGGGGCGTGGCCAGCCCCCCCCCCCCAGTTTGTATATAGCTGTGTGTGTGTGTGTCTCTGTGGGCAAAAGGGTTAAAGCTAAAAAAGTATAGCATACACAAAGGCGCCATTGTGCCATAAACGACCCAAATATTTCGGAGGCGCCTAAGCTTTAGTCTCGTTCCTAGATTAATTATTCATAATACTCGCGGCTCTCGCTTTACCGAATTTGCATTAAAATGCATAACGGCTTTTAATTAAATTTATGGCTGGCAAGGCCACTTCAAAGAAAGGCCCGAGGAAATATTAAGTATACGCAGGCTAAGCCGAAAGATGGATTAGCAAAACAGAAACGTGCAGGTACAAAAAGGATGCTAAAAACTTTGATTTCTTTATTTATTTTTATAAATAATTAAGTATTTGAAAGGCAGATAGTGTTTTTTTGAGTATCCATAGTGGCCCAGGACC GGCACTCGCGAAATTGCCCACTCATCTTGACTAATGCTCGAAACTCTTCTTTGGCATTATCCATTTGTTTGGCAGCCGACTCAGATGCCGAAACGGACGAGGACGAAGGCCATATCGAGGATGCTGAGAAGGCACAGCAGCGCGACCGCGAGAAGCACGACGAGAGCGAGGAGCTAAGCAATAAGGACATCCAGGAAAGCGACGAGGACGTGGCCGTCTCACTGCTGCGCGCCAAGAACCTATCGCTGCTGCAAAGCCAGTAAGTTAGCCCATCGACCGCATAACCCGAAAATGAACACCTCACTTTTCCAGGGAAATATCATCTAGCCAGCAGAACTTCTTCCGCATGCTGGACAAGAAGATTGATGAGGTGAGAGTCCACTTAGTGATACATTCCCACATACCAGATGGGAAATTTAAATCAAGGGGGTTCCCAATAGTATAAAAACTTAAATCACTCGCTATAAAGCACAGAGAACAATTGTTGTTTCTATAAACACAAAAAGTTGAAAGTACGAAATTCTATAAATTCATTGACCCCAAATACGTAATTTAATAGAATTTATTAATTTTAACATACATATATATTTTAAAAGCTTTGCAATATAAACTCAACCAATGTAAATAATGATCAGCTAACTAGTCATAACATTGATGTGTAATTGGCTGTTCCGTGATAATAAACAAATGGCCAAAAATCAATATATATGTAAGTGCACTAAGTATTTGGCTATCCCAAATCGAAAGAGCAAAACAATTAGTTTTATGGCCCCCTATTTGTTTTCTTTTCGAACAACATGTGAGGAGCCATTAAAATTCGCTTGTTACCTGAAAATTTGACTTCAAAGAGATTGGAACAACTTATGCGCAGCAAGAAAATGTACATATATGCCATGGGGATTAATTTGATTATTTTATTAAACATGTTAGACACAAAATCTTTTATAAATTTCCATTTTAAAGGTATTTTAATTTGATCTTTATATTCTGGGTGTTTTGCTAAACTTTTACTTCTTGTCTGTGACCAAATCTGGCTAAACACAAACACGCTTGTTTTAGGGTCCCGACTACGATTCGGCCAGCGAGACGGAGATCGCATTG
 
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