CR60183

CRIMIC CR60183

Gene CG5644

CG5644

Information

Donor Location Cytology FBti Stock number
pM37_p1 3L :..Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK OK

Images

Construct Design Sequences

sgRNA sequence ACAAACTGTGAGAGCGGCTGGGG
sgRNA2 sequence
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
 
Amplicon 5' Amplicon 3'
GATTCTCCTTCTCAGCGGGTTCTCGTTTCGATTCTCACTGCTATTTGGGCTCTCTAGTGAGTTTCCATTGCACTTGGACTGCCAGTCGACGTTTGGCAGTTCGGCGGGCGGTTGGTTTGCTGCACCATTTTGCACACGTTTCGCATAGGTGTGCTATAACCAATTCTTTGGCCAGCGGAAATTGTATAATAATTACGGAAAAAAAAACTGACCTGGACTCAATTTGGATTTGTGTCAGTGCTAGAAAGAAAATGCTGCAGCGGCAGAAACCAAGTTCTAGCAAACGATTTGGCAGCAGCCCTCCAACACGAACCATTCCTCGTCGGACAGCCAAGTGAAGTGGATAGAATGGCTTTGGCAGCGGCACCAACAGCAGGACCTTCGCATGGCGGCTTGGCATTGACCGTGAGTTCGGCCGGTGGCTACGATGGTACCAATCAATACGAGGAGAACGCTGAGCACTTGCGTCAGCTGTTCAGCCAGAGCAAACTGGTGTCCTTTAGCATTGCCCACATCGCGTGCTCCGCGGGCAGTAGCAGTGGCGACAACTATATGTCCGTGGTGAAGCGTGTAACCATCTCCCAGGCAGGAAAGGATCAGGATCCGGAGCTGGCTGGCAGTGAAATCGGTAAAGTGGTTCAAAAACATTTAAGTCCATTGCATTGATTTTCTCTTTAATACCCTGCAATGTGGCTTGCATTAAGGGTAGTACTCTTTGTTAGATCGCATTGTTTGAGAAATTTTGTTGTGCAATATTTAGTTAACGTTGAGATCTCGTTTAATCGAGTTGTTGAAATATTATGTGTACTTGTACCAATAATATTTATTTTGTACCATGAATAGAGTATTATTCAGTTCAAACCTTAATTGCAACGATATTAGCTGAAATGTTTGCCCGCTGAGCGGCGGCGTCGAGTGGGTTGACCTTGTTACACGCATTCTAAATGCATTGCACCCATACACATAGGCATATACGTAATATAGATTTGCTAAAGGTCAGCACGCACACTCACACACGCGCGATAGAGAGAGAGAGAGAGAGGCGGCGAATAAGAGTCGCCACTTTGCTCTCACG GTTTCCTCACCCACCCCCTCTTTCACAAATCATTCTCAGATAACACATCTCTTTTATGATTTGATATGAATCATTGTTTATTTAACACTGAATTTCGATAGAGAATGCAATATTTAGATCATATTAAATTGATAATTAAACTTTTTTTCTGTTTAGTCACGGTCATTGTGAAGCGACAAATTGCGAGCCTTTCGCGCCGGCAACTCTACCGATGCGAGGAGGCCTTCAGCAACGAGATCAACGCCTATCGGCACTTGGCGCCCCTATTAGCTGCCCACAGTCGTCATCAGTTGTTTCCGGTATGCCACATTGCGGAGAGTCAAGATCGGCGGGATGCGGAAGGTGGAGAACCCATCATCGTGCTTCAGGACCTCAAAGCCATGGGTTTTCGCATGAAGGATCGTCTGGCGGGCTTGGAACTGAGCGACTGCCTTCTAGTGATGAAGGTAAGTGAGTGAAAGAATTTACTTGTAGTCATTGAGCCATAGTTATTGAGTGTCATCTATTTGTAGAAACTTGCACAACTCCATGCTGCCTCGCTGGCCGCTCAAGAGCTGGAATCCTCTTCCTTCGCCTTCCATGCTGATCATCTTCAAGAGATTGTCTACTGCGATGAGGCGACGGACTTTTACGCCACCATTCTAGACACCTCCGTGCAGCAGGCTCTGGAAAGTCTAGGCGACGCCAATGCAGACGACTGCTTGACCACGCCCATTCGATTGCTCGAGGAACTTCGTACCAATCTGTTTGAAAATCTAAAGCACGAGATTAATGCCACTGCGGCGGCTCCAAACAGTGTGATCTGCCATGGTGACCTTTGGGTAAACAACATTATGTTCCGCTCTGAGCCAGAGGAGGTGATCTTCTTCGACCTGCAGGCCATGCGCAAGTCGTCGCCTATTTTTGACATTCTCCACTTCATTTACACCAGCACGAGAAGGCCACTCAGGGATGTGCACACGGACACCCTGCTGGCCGCCTACTCGCAGGCTTTGAGTGAGGAACTAAGGCACCAGCTGGAGGATACAACCGCTGCCGAAAGG
 
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