CR60241

CRIMIC CR60241

Gene CG30116

CG30116

Information

Donor Location Cytology FBti Stock number
pM37_p2 2R :..FBti0219192 Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK OK CCAGCTATGCCCAGGAATTGTG CCCTGAAATTGGTAATAGCCCTAA

Images

Construct Design Sequences

sgRNA sequence TTACACATGTCGACCAGTTAAGG
sgRNA2 sequence
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
 
Amplicon 5' Amplicon 3'
TGAAAATGTGCCAAAGCTGCGGGCTGCTCGAAGTTGACTCTGGTAAAACCTCGACGAGGACGACGATGCCACTTGATTTCGGCCAACTTAAATAAGCAGGCAAGGCGAGGCGAGGCGCCACCACACCACCCACTCAATCACCGACACAGCCGCGCGCAAAAAAGAAACAAACAATCAAATAAACTAGGCAAAAAGCAGCTAGCAATCGAAAATGGCCGCGCCCATCGACGAGAGTGTGCTGAATGCACTGAGAGGCGTAACCAGCGCCCACACCCGCCTGCCCAAACCGCTGCTCATCAAGATCTACGTGGCCAGTCTGAAGCAGGAGTTTAACCAGGAGCGACGCATGCTCCTCGAACTGGTCGGTCCCGAGCTGCAGTCCCTCTACGATGACCGACAAATTGAGGTGGGTGCAAATTATATTATTACTGGTTTGATGTATTATTTAATTTGATTCGATTCGTAAACAGCTGGAATTCGTGGACATGCACTTCGGCACTGGCGACCTGGAGGTGCATCAGCTGGAGCGGGATCCCTACTTGATCCATGACTATCTGCACGAGATCGACACTTGCCACGCGCACACGAAGAGCGTCTTCTTCATGGTGCTCGTGGGCGATGGCATCGGTCGTCAGCTGCTGCCCACGAAAATAGATGAGGACATCTTTTCCGCAGTCCTCGCCGATCAGCAGACCTCTGCCGATCATGAGGCTATGTTGGTTAAGTGGTACGAAAGGGATGCAAGTCAAACGCAACGGCAGCTCAAACAGGATTATCGGTAAGTTAACGAGTCGAATACTTAATCACGTTTAATTAGACGACCTAAACAATTTATGAACCGTTTTGCATTGAAGAGAAGTAAACCAGATGTTGTTTATTGATCGGTTACACATGTCGACCAG TTAAGGTTGTTTTGTTCTTGCCTGCAAGCAGCGTAATCCGCAACTCAAACTGCAACCGAAATAGTTATTGGACAAAGTTGGCCAATATTTGTCAAAGTTCCGTTTCCGTTCCATTGTGCCATGTTTGGCTGAAATTATCCATGTGCCACATTCGCTTTGTTTGGCCACTCAATTGGAGCTGAACTTTGGCAAATATTTGAATGGGCGGAATGGGGAATGGGTGTGGTAGGGAAAATATGGAAATGCCAACACTTGCCTCGACATCTGGAACGTGTCCGCCAGTTATTGAAACCCCTTCATTTTTTTTTTTTGTTGCAGCCTTATGAACGTGGATGCCTGGCTGGCTGAATCACAGCGAATGCAAAGCTTTTTGGAACAAGCCTTTCAATCGTTGCTTCAAGGATCCGGTGCATCAGGACGATCGCCTGACTTTATGGAACGGGTTCAGCTACTTCGACGCACGCAGATCGAAAGGGAAGTCTCGCAGGCTATGGGTAAGTTGGTTTTTTATTTGTATCGAAAGAATATTTCATATTCTAAATTCACGTTTTCAGCCCTCACCAGCGAGAAAATCCTGGCAGTTTTCCGAGAACGAGCCGCTCAGTGCAGCAAGGGGGACGTGGAGGCGGCAGAGCGGCTGCGAAAAATCAAGGACGAACTCACCATGAACCTTTCCACAGATAATCATACTACACTTGTGTAAGTTTTCCGGCTGCATATATGTGTCAGTTATTTCATTTATAGTCCAACAAAGAAGAGTAACTTTTTGATAACAAAAAAATGCAGTATATCGTTAGTCTGATGTGTGCTCACTTACGAAATCCACTACTATCACAACTTTAAAAACTTTAATTAATCACTTTTGATAATGCGAAAAAGTTTCATTATGATAACTTTTACCAACCACGAATAATAAAAAAAAAGTGAAAAGGCATTATGCAAAGTGCCAATTAAAGCATTAAAGGCTCAGCTAAAACATTTTTTTTTACTGAAATATTGACAAGAATTTAGTGAAAAGCATTTTATATTCAATTATAAAAAGCTAAATAGATTCTTATGACGTGCTATAACTGAAATCTAACGATGTCACATTTAATCATTTTTTATATTATTTAATATTTTGTGAAAGCACTAGTCAGAACAATTGACTATTTGAGTCTACACCCAT
 
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