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CRIMIC CR60245

Gene Cpr62Ba

CG13934

Information

Donor	Location	Cytology	FBti	Stock number
pM37_p0	3L:1824129..1831783	62B6-62B6

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TACGGCTTGGCAGCCTTTCACAAA	GAGCTAGCATCGGTAGTAGCTGAT

Images

Construct Design Sequences

sgRNA sequence	GAAAAGGTGCATATCAGTTGTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right

 

Amplicon 5'

Amplicon 3'

GAAACGTGCCTCAAACAGCCAAAAATGAGAAATAGTTAGCCATCATGATGCAAGAGAAAGAAAGTTGCCACTCGCGGCTCATAAACGGCAATCGCCTCGAAGACAAAGCCAAGCCCATGGATCGGGGTGTTGGGGCACCAAAAGGGTTCAGGTGCCTGCTCTGGGTCAGAGCAATCCAATCGGGCGGCCAGTCACGTTAGGGCATGTTAGCCAACCGTCGGAGATCGCCAAGATCCATAATGTGCATATTGCCATGGGTTAGGGGCCTTTGTCTTTATCGACTGCTCGCTTTTTGACACTAAATTGCCTTGACTAAGTCTCTTGCCCCTAACGAAGTGTGAAAGATGCTGGTCTTTGATTCGCTTTGATTGCCCAACTTGTGGCATGCACCACCGGTCCACCGGTCCACCGTCTAATCCACACCAGCGTTTTTGGCTGCTGTGCACCGTGAAGATGTAGTTAACAACGTGACTATGTAATCAGCATCGCTCCAGCCGCTAGGAAGTGGCTGCTCCGAGCTTTCAACTGCTGCCGTAACAGCAAAGTGCAAATGATTTTCACCCCGACCACTCCTCCGGGGCCAATCTCCGGTCAGCACTGGGCGCCCCCTCATCTCGGCCACTTCGTTATGTTTCTAATCAAATTAATTTCGGCAGCAACTCATGCCCACGTGTCCGGTCAGCATGGCATATGGATGGGGATTCAGCTTCGTGGTCCTCTGTCTGCTGCATATTGCGCATACGCAGCGTGGTCCGGCTGGGCACATCGAGTTTTACAGCGATTACGGCAGAAATCATGACGAGGAAAGGGTGAGTCTGGCGCCTGTTTCACAATGAATTGTGTTTAATAAAAGCTTTATGGTAGTCAACTAACACAGTAATACAAAATGCTCCTTTTTTGATGTATTTAATAAACTTAAAATGAATATCTAAGAAATCCACAA

CTGATATGCACCTTTTCAGTCTACTTGTTTAATTTGCACTTTCAGCCGATTTATTTATTGCCTTGTGTGTTTATCTACCAACTCATCAGACCTGATAAATATAATAATTAGCACTGCAAACATTGTTTTTCTGTATAACAAATATCGTTTACTGTAATGACACAACTTTTAAGTGACACGGATCGGAGAATAACATAGCATCTGTCTTATTCCCAGCTCCACTACTCGCATCAGTACCACATCTCCGACGTGGCCAGTCGGGTGCACATCCTGCACCGAGAGCAGCGCCATGGTGACTACGTTTCCGGATCGTACTCCCACCTGGAGCCCAGTGGACACATTCGCAGTGTGCACTACGAGGTGCGAGGAGCCAACCGGGGATTCAAGGCCGTTGTTGAGCAGCGAACCGGGAACAGTCGGGTGCACCAGACGCTGGAATTCCGGAGCAGGCAACCAATCCGGGCTCTGGCAATAGCCGAGCCTGTGGCATTTGTGATTTAAGGCGTCCAGCGGCGTAAAACCGGCCAGTTAAAGCAAAAAAGCGACAAGCGAATTATTTGCCTTGTTTTACGCAGCCGTAAATTGCAAAATGTTTAATTGTCTTTCAATGACTTCGCTGACTTGTTAAATGAATTTCTTATTTGAAATTTAAATTGATTTCGGGAGGGCACCATAAAATGTAAACGAACAATTTTATTAGACTTAAAATGTAAATATTTTGGGGCTATAATTTAAGCCAAGCGGAATGAATGAATAAAATTATATCCCTTTTGTTTTCGGAGACAAACAAAC

 

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