CR60246

CRIMIC CR60246

Gene fax

CG4609

Information

Donor Location Cytology FBti Stock number
pM37_p0 3L :..FBti0219195 Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK OK GCACAATTAGTCATCCCACGCAAC CTATCCTTGTCCTTGGTGTGTGGA

Images

Construct Design Sequences

sgRNA sequence GCTGGCGTTGTGGATTAGGCGGG
sgRNA2 sequence
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
 
Amplicon 5' Amplicon 3'
TAAGATGCGTTTCCGCTCCAAGAAGGGTCAGCTGCCGTTCATCGAGCTGAATGGGGAGGAAATCGCCGATTCGGCCATCATCATCAAGGAACTGTCGTCCAAATACGAGAAGTACCTGGACTCGGGACTCACCGCCGAGCAAAGGAATGTCTCGTACGCCACGATTGCCATGCTGGAGAACCATCTCATCTGGATCATCTTCTACTGGCGCGCCAAGTATCCGGACAATGTGCTCAAGGGCTACAAGGTCAACTTGCAGCACGCCCTCGGCCTGCGGCTGCCCAACTCGATTCTGAACTTCTTCTTTAAGATCACCTTTGGTCGCAAGGTAACTGGATAGTTTGTGATATTTAACATACATAGGTCATGATCGTGGTCCACGATCTTCACTTTCCCCGAATTTGTATGGTTATGATTAGAATTTTCATTGGAACATTGCATACTTTTGGTCATTATCGTAATTGGTTCTACAGAAGTTGTTTACTATCATTCATACCTAATATTTCCTTAATATATCAACATCTTTTGACACATACTTCTAGTTGAACAATAAAACAAATTTATAGGTTTTATTGTTCGTAAAAGTAACATTTAATTACAAATAGCTCATACTTTGGTACAATTTTTTATTTGTACATTTCATAAATGCATATTAAACAATTATATTCTTTCGTGATCTTAAGAGTTGAAGCTTTAAGTGAATATTATTACCATTTTGTTGAAAGAGACTGTACTCTATCTTTAACCAAAAGAAATCTATTGAACTCCAGTGTTAATCGAAATCAGACAAACTAAGTGAAAGTGGAAACCGGAAGCTAGTCAAGTAGAGAAATGTTTTTAGTGTAGCGGATTTAGATGTTTCAGAAACTCAACGCCAGCAGCTGAGCCTCCAGTAGTTCCAAAAACCGGAGATATCCGCACTTTGTTTAGATTGAAAACGTAATCGAAAGCCGGGTAGGTTCTCGAGTTGGCTGGCGTTGTGGATTA GGCGGGGATGGATGGGTAGTGTAAATAAACAATACTTGAGAATTAAAACTAAGCAAGCGGTGGCGTATTATATTGCACCTAGCTGTACATAAAAAGAAAAGAAACTCCAAACACACCACCACCATCCCCGCACATAGTTTATCCCGAAAACCCCGATGGCATAAACAATAATTCTCTGGCGGGATGCATTTGTTTATATCAAATCGATCGGTTCTCACATAACCAACCCAAATCAAAATCAAAACCCAAACGGGAAATCGAAAGGAAAACAATCATTATTTTACGACTAACTCTTGCATGGTTTTTCTCTTTTTTCTTTTTCGTTCTTTTTTGCATACTACTTTGGTTTTGAACATCTAACTGTGTAATGCGTAATGCCCCCGATCCCAAAAAAAAAAAAACTCTAAAAACTCACATCACCTCCACCACCCCGATTCCGTAACGTATGCTTGTGCATCCCCCCGTAACCCCCATCCGTAACTGCTGTACGGCAAAACAACTATTGAACACAACAAGTGGTTCCAGGGCACGAAGAAGCTGAAGGCGCACGGCATCGGTGTCCACAGCGCCGAGGAGATCGAGGAGTTCGGCAAGGACGACCTGAAGGTGCTCAGCGAGATGCTCGACTGCAAGCCTTTCTTCTTCGGCGACGAGCCCACCACCCTGGATGTGGTGGCCTTCGCTGTCCTCTCGCAGCTCCACTATCTGTCCAAGGACATTGCGTATCCGCTGCGCGACTACATGACCGAGAAGTGCCCCAACTTGATTGGCCACGTATCTCGCATGAAGGACAAGTGCTTCCCCGACTGGGACGAGATCTGCACGAAGCTGGACCTCAATGCGCACATTCCCAAGCCAGAGTGAGTAATCTTGGATGAAAACTTTCCAATGGATGATCGGTGTACTAATTCATTTTTCCCGCTTTTCCGACAGGCCCGAGACCAAGGAGGGCAAGGAGGGTGGCGAGCAGGAGAAATCAAACGAACAGGAGGGCACTGAGGGCGACAAGATCGAGAAGGAGTTGGAGAAGGACAAGGTGAGTCTTG
 
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