CR60249

CRIMIC CR60249

Gene Cpr76Bd

CG9299

Information

Donor Location Cytology FBti Stock number
pM37_p0 3L :..FBti0219197 Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK OK GAGGCGTTCGCGACTATGATCTAA TGTAACCAGGAGAGCTGTAGGTCT

Images

Construct Design Sequences

sgRNA sequence AATAACAACACATTCAACATTGG
sgRNA2 sequence
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
 
Amplicon 5' Amplicon 3'
ACAATTGGCCAGGCAGGTTGCAGTTCGCCAGTTTTCAGCTTAAGTTTCAGTTAGAGCTTGCAGTTTGCAGTTTTCAGTTTTCGGTTTTCGGTTTACGGATTGGTGAGAACAAAGCTGCTTTGCAACGCGATCTGGAGTCGAACAGTCATCAATTCTTCATCTGCACGCGCCAAAGTCGCAGCTTAGGCGCAACTCACTTGGCTCTTTTCCTGCAGCTAGATCCATTTTCTCGCTTTTCGCTTCGACCGCTTCGATTCGATCTCAGCCAAACACATAGTTACACTTTTTTTTTGTCGCTTTGTCGCCAAACTGGTGCGTGGCTGCTGCGCATGCGTGCGAGCTTTTCGCGACTGTTGGCCAACTGACCGAACGTATATAAGCGATTATAGGGCCGGGCTGGCCAACAGTCCAGTCCAGTCGGCCGCGTGGCTCTGCTCTACATCCCGAATCGTTCCGTTTCGATCCGTTCCGATCCGATCCGATCCGATCCAACCCGATTCGATTCGATCCGTTGTGTTTCCTAGTAGAGTTTAGGGAAATCGGTGATCAGCTTATTGTGATTTTCAGAGCCAGACAAGGATAAGCCTTTGGATTCCCATCACCAAGGATCGCAGACTCGAGTTTTCTTTTTAGAAAAGTGCAAATGTGGCGTTATTAAGAAAACATCCTTGCTCAACCGCTCGCGAGTCCTTTCAAACGAAAACCAAACATCGACTGTGCAGAGTTTGAAAAGTGAAAACATGAAGGTGGGTCCTTTATCTAAGTATTGCGACATACTGACATTTCAAAAAGGACTATGATTACCATTACAGTCTTAAAATCGACTATACTACATGTTGTTTGTGTTTCAAACACAATGAAGCGTGATTTAAAAAAAAAGGACATGCAGTGGCCAATG TTGAATGTGTTGTTATTCTTAATTGAATAATATTATAAACCAAATATGGTTTTAAAACTAAAATCCTGCTAAACGTGATTAAAAACCATTGACAATTGAGTTGTCTTGTTTATATTATATTAAAAGAGTGCACACATTATAATCGAAAAGCATAACATGGATACATTACAACAAAAGATTTAAAAGTGTACACTTCGATTTTGTAGATTTTTTTACATGTTTAATATTTAAATATATTTCTTAGCTACAAAATTTTCATTCTTGAATTTTCCTTTGTTACCGTGAATTTTAAACCCATTTTCCAAAAAAATAATGTAGGAAAACAATTACAATTTATTTTCACGTTTTGTTTCAGCTTCATCTGGTGGCCCTCCTGGGCATACTCAGCGTGGTACTTGCGGATCCGTCGCCCACCTTGGCTCCACCAATCTTGCCCTGCGTACACGCATCCACTGCTGGTTATGCCTATCCTCCACCGGCGGAGGTAAAGTTCTCCATCTCGCCCGGAGTGACGAAGTACAGCCAGAGCCCGGCGATATCCACGTACTCGGAGAACGGACAACTCCTGCACACCTCGGTGGGAAGTTCCGGAGCCGCCTATCAGTCTACGGCCGGAATCGAGGGCTTGTCTCACGGTGGCATAACTCAGATCAACAAATACATAGCTCCCGTGCAGAAGAGCCTGTTCTCTCCCTCAGCGGAGTACGGAGGAGCGGGAATTACCTATGCGGACAAGTCACCAGCGGCCAAGTACGCAACCGTTGTGCCGTCGGGCATCGAAATCAAGAACCTGGTGTCGCCGGCGGTTACCAAGTTGGACAACTCCTACTTGCCACCATCACCCGGAATCACTAAAGTGGCGACATACACCTCGCCCGGATATAGTTACAGTTCGGCAAGTCCGGGAATATCCAAAGTGGCGACTTACTCCTCCCCATCGCTTTCCAGCGTTCCCTACTATGCGCCACCAGTGACTTCCAAGGTGGAGACATACAGCTCACCTGCGTACAC
 
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