CR60251

CRIMIC CR60251

Gene CG5196

CG5196

Information

Donor Location Cytology FBti Stock number
pM37_p0 3R :..Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK OK CATCCTTCTTGAAGGGATGGATCC TGATGCTGAGCAGAGTGAACTGCA

Images

Construct Design Sequences

sgRNA sequence ACTGCCCAAGTACTTAGTCCGGG
sgRNA2 sequence
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
 
Amplicon 5' Amplicon 3'
CAATTACGTGGGCGGCACTTGCACCGGCAACGCCCCACTCCGGGCTATGTCTGCGCCATATCGCGTTTCATATGCCACCCACACCAAACGCAGGAGTTCCAATCGGTTGTCCACCCACCAGGGACTTTTAATTTGCCTAATGTGTCGTGCTAATTGCGTTTTAGTTGTGTGTCGTTTGCCCTTTTATTGCCCCCGGATAACCCTGGTCCTTCGAGCCTATCGGGAATTTCACGGAGCCACCACCAAACATGGCGTGCAAATCGGACGTTTGACTGCCGGCGGGTCAGACGGCATAAGACTTCCGGACCACTGCGATATCGAACTGATGTACCATTGTTCTCGGCATAATGGCTTTATTAGACGGCGGTCGCTTGTTGCTGCTCTCGAAAAGGACTCGGCGGAATCAGTCCGAGAAACGGCGAAATTACTCATTACTGGACCGTGGAGCCGGCTAATCAACTCGAAAAGCGACAATAAACTAAATAAATGACCCTTATTTCCTTCGCACGCAATTGCCAAGCGAAGCATGGCCACCAAAGTCACCCCCATCCAAAACATAAAAAAATAAACAGGGCTAGGTCCCTCCAACTACATACGTGTATCAAATTTTCGTACATGAATCACACTAATGTTTATCTACCTCTTGGCTTTCAGGCATCATCAAGTGCATAACGCTGACAACGCTCTATATGAACTCGATGTGGTGGCCGCCAAATAAATCATTCGCCGGATTCGCCCACCAGGCCCTCTTCCTGCTCCTTTCCACGCTGGCCACATTCAATTACGTCATGGCCACTTTGACTGGTCCCGGACTGATGCCAAAACAGTGGCATCCCAAGGTAACCGGAGAACCAGAAGCGCACGGCACACGGAATGGTGGTGAAACATTTGTTGTGACACGATGGCCGGTCAACCGCCTCCGCCTAGTTCGAGACACCTGTTACCTGTTTCCATTGATAGTGCCCACCATTTGGCCCGAAAAACTGTTGACCCGTCACGAACTGCCCAAGTACTTAG TCCGGGTGTGGTTTCTCCGTTTCTTTGTGCATCGTCATTCCACGGCGGAAGCAATTGTTTAGCGTCCGATGAGCAGGTGCCGAGTTTTCCGGAGGAGTTCGCTCTCAACGGGGTCCGATTTCCAGGCCAGACAAATGGGCCAAGCCGGCCCATTGGCGTGTTTGACTGCTTATCGCGCATAATCCGTGCCAATTAAACGCTGCAATTTATTTTAAAGAATCCGGACCCGGTCCGTTGTATGCATAGATGTCGTGCAGGGGCCACCAACGGCTGTAACGTTTTTCGACCGCCATTCATTTTGTCCGGCCGTGGCAGTAGTGCGACCCAAATATAATTTATTAGCGGCGACTCCTCACATTACGGCCGGGCAGTTCGGGCCCTGCACGCGAACGCACTCTACGGGGCCCAAAGTCCGTAATGCAATTACCGGAATTTGCATAAATGTTTTGTAGGCGAGCCCAGACGCAAGAGGGGCGGATTTCTTTGGCTAGCTGCGTGGCTCATGGGCGATAGAAGGTGGGTGGGAGGTGGTCGGTCACCTAATGAGCTGTGCGTCGTTAGGTTTTGTTGTACCTGACGGCATCGGGAAACGCGCATAAATCACGAATAATATACTCGTTTTCGCTGTGTATTTTAATGAATTTTAATTTTTGTCAGGATCCCAAGGACGCCCAGTTCCTGCAGTACTGCAAAAAGTGCGAGGGCTACAAGGCACCACGCTCCCATCATTGTCGCAAGTGTAAGTGATATTAAGATGATTTGCACAGGAATTTAAAAGAACTTAAGCAAATGAAAATTGTTTAAATCGAACACCATAATGATTTATATATCTGACCACTTAGGTGATCGCTGCGTCAAGAAGATGGACCATCACTGCCCATGGATCAATCACTGCGTTGGCTGGGCCAACCACGCGTACTTCACATACTTCCTGCTCTTCTCCATCCTGGGCAGCCTGCAGGGCACCGTGGTGCTGTGCTGCTCCTTCTGGCGGGGAATCTACCGCTACTACTATCTCACCCACG
 
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