CR60252

CRIMIC CR60252

Gene CG7518

CG7518

Information

Donor Location Cytology FBti Stock number
pM37_p0 3R :..FBti0219198 Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK OK AAACGAGCGAATTGCAACGAGCAG GTAGCCGTCCAGAATGTCTACACA

Images

Construct Design Sequences

sgRNA sequence TGTACTATTAAAGATGTGTGTGG
sgRNA2 sequence
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
 
Amplicon 5' Amplicon 3'
GTCCCGGAAAATCCAAACGGATGGCCCTGCAGTGAAACGCTGATGAAAGCAGAGGCGCACTTGGCGCGATTCGAGGGGCAAAGCGCGTTGCGTTGTGCTGCTCTGCGTTCTTTTCCAAAACCGATTTCGATTCCAAAAACCAAACCAATCCAATACCCACACTTATACACTTGCACACCGACACACCAGCGCACACACACAGACACGCGCGCGCATACAGTGAGCGAGTGAAAGTGCATCTACATCTGTGATTTCGTATATAATAATGCGTCGCGCATACGCGGATTTGGCCAGCAGAAGCACCAAAGACTAACAAGTGATCCGGAGCCGGGGACTGACTGAGTTGGCTGGGCTCATTGACCCATCCACCGCGTGAAAGCCCCGCCAGAAATGGCCGATCAGGTGGCGCACACCAAAGAAACGGATGCAGATGAGCCGCTGGTCAATGGCGAACTGCCAATGGAGGAGGCGCCGCCTTCCTGCTGCCATGTGATGTCTCTGTCCTCCATGGACGTAGATGTAGATGCGGATGCGGAGGGCGATGTGGATGCCACAGCGTCCTCCTCCTGCGTCGCCACCGCTTCCCAGCTGAATCCGTCGCCACCGCCTGCTGGTGTCCAACAGGCGAAGCCGGCGCGGAGCAGCTCACCGCCATGCCAGCTCCTGGAGCGGCTCAGCTTTCCCGACGGCAACATCATCAACACGCTGCACACGATCCTCGCCCTGCCTCCTTTTGATCCTTATGCCCCCGCCCCCAAAGCGGAGACGGTCTACAAGGAGGTCGTATCGCTGATGCAGTGGAGCCAGCGGGAAGACAACGCCATCGAACTGGAGCTCAGCGACAAGCTGCTGCACGAGTGCGAGCACAAGGGCGGCGATCCCAACGATGAGACGCGATCGTATTTGGTGGGTTCCCAGAAGCTGCCCGAAAGCTGTGGAGATCGATTTCATTTCATGTATTTTTTTTATTTAAATGTACTATTAAAGATGT GTGTGGGATTTTTTTATATTATACCTACATTTAGATTGGATTTAATGAGTTATCAAATCAAATGGGATAGAAATTATCTTATTAGATCGACTTTAGATGTTTCGAAATACTTAAACATGATTTTTTTTGGTTTAAAGAACACTTATTTGAAATTATGCTTTAGAATATTGATAGGTGCAATCATACCAAATACATTATATTTGAGAATATAAAGCTTTCTCAGTAAATACTCACTATCGTTCACCTTCTTTTCAGATGGCCATTGAGAACATGCGCCTGTTCTCTGAGGATCCAAAGAATCCGCTTCGTGACGCCGACGGACAGCTGCGTGCAATTACGGTGTGTTTGCTTGAAAGGATTAAGATGTATAACGAAACGTACTCCTAATCCATTGTAGCTATATTTCCAGAATCTTGCCTTCATATTTGTGAAACACCGGAAGGAGGAGTTCTTCACATTTTGTTCCTGCAAGGAATGTCTCGAATACCGCGAAACCATCATGGCCATCATGATGGATCAATTTAAGGCTGCGCACATGGTGGTCATGTTGCTAGACGTACTGATCAAGGCCTACAGTCCCATGTTGAAGTAAGAACAGTCTGCAAAACTGCAATAAGAAAAAGTCTCCTAAGTCAGTTGGTAATCATTTAAACATCTCTGTTCCAGAAACGATGCTGCCTACAATAAGTTTGAGAAACTGCTTGAGTGCAACAAGGAGATCTATTGGATTACGAGCGGTTACCTCTACGATAAAAATGCCGAATATCTTGATTTTATTGATGATACAGCGATCTCCGACAATATGATATTAGTGCTATTCAGCGCCATGTAAGTGAACACCCCAGTCCTGCATTTGTTTACTTTAAACGCATTATCTGTTCACAGATTAATGGCTAACAATCCGATGCTGCTGCTACAAATACTCGCCTATAATGTTGAATGCATTGTGAAAGCGTTTTCCGAGGGCATGATCGAAATTGCCCAAAATATTCAGGAAAACGAGAAGATTTCGGCAGAAAGGATGCTGACATGTAAGGGAATTCTCAAATGATAGCTAACTAAATAAATAAGGGG
 

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