CR60253

CRIMIC CR60253

Gene yellow-f2

CG8063

Information

Donor Location Cytology FBti Stock number
pM37_p1 3R :..FBti0219199 Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK OK CCGTGTTGTTGATGAACGTGCTCT GGCATTGGAATCCACAGAGCCATA

Images

Construct Design Sequences

sgRNA sequence ACCAATAAATCTATCTAACAAGG
sgRNA2 sequence
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
 
Amplicon 5' Amplicon 3'
TCCGTTGCGCTGGATCTCATCGAAGAAGATCACCCCAGTCCCAGGATCGTAAGCGTGCATCGTAGACTGGGTGGACGGACCACGGCTTCCGAGTACCCGGAAATCGTTCCCATGATCGGAACGTGCGGCGTTGGATTCCTGCTGCAGAACCCGGTTGGAGACCACAAACTCATTCGTACTGGCCATGGGATGGAAGTAGGCATCGCGGCTCCCATCCAGTTTCTGGGCCCCCAAAGTTATTGAGAAGATGCCATCGTCCCAGCGGAAGGTCTGGCCGCCAATGCTGAGATCCCCGGACAGCGGATCGAAGTTGAAGTAGTTGTGCTCGAAGGACCAGATTCTATCGTTTCGCAGGTGATAGACGTACAGGCGCCGGTACACCAGATCCGGAATATAGGCGTAGGCATCGCCACACTGACCCGCCTTCACATCCACGGTGATGCTGGCCAATCCGCGACCCGTCTCAGCTATACTCTCCGGAACATCGAAGCGCTTCAGCACCTGATCCGTGGCCAAGTCAACTACCCAAATGCTGGGACGGCGGATCTGTTGGCGGTTATCTGGATATGAGAATCATGATTGAGTTTTTTATATTCTTGGTCACCAAATTAGATTAATACGGATCGATCCTGATCTTTTATATTACATGTCAATACATGCCAACTCACTTGGATACTCCAGCATTCCCGTGTCGATAAACCAGAGACGCTGGCATGCATCCACGGAGGTTCTGTAGACGGACACCAGATTCTCCGCACTCGCGTTGAACTGATTAAGCGCAAAGTTGGGATAGGCCCGCAGCTTGGGGCTGCGATTCGATCCGTCCTCGGCCAGATCGATGTAATTCAAGGTGGATGGGATGCCCACCCGTCGACGAGGCATCGTGACAAAGAGGCGGCCGCGGAAGTGAGTGGCTCCCATGGGCACGTTATTGTACGGAATGTAGGAGGCGTTGGGGTCGTCGCTGTCGGCGCGGGAGCTGACCACTACCGGTGTATCGCGGGAGGTCATAATCTTACGCTTTCGACGAGAATACTTTCCAGGAAAGACAACCGGAGCTAGTTGAAC ACAAGGCTCGCGAGATTGCTATTGCTGAATGAGAACTAGTAATATCATATTATGAGTACTAACCTGAAAAGCTGGGCCTGTCTTTCCTTCCGCCCGAGCTTAGATGGCCATCTCCGCGATTGTAAAAGTCCATCTGCTTCCATCGGAAGACCTCTATCATGGGATCAGCATCTGCCAAGGAAAGCAGCTGTAACCCTGAGATTAAACTTAGAATCAAAAATTGGGGCGATAACATTGCTCGGTTTTCCTGGGGAACTATAATCTAAGTTCGCCTTGCCAAGTTGAGGCCTCAATAGCGACTGAAAGCCAATTTGTTCCCAATTGGAATCTATTCTCACAGCGGTTCGAACGCAAATTATTAAAATTGATAAGAAAAGCGCGACTCTCACAAGTTCTCAAACCGGTTTTGTGACTTGTTAGCCTGATAATAATCGTTAGTAAAAATCTAATTATTTACATACAAATGGAATTATAAACAGCTTTCAGCTTTTCTTTCTTTTTATGTTGTATACATATATAAATCAATCACAAAAACATGAGAAGAACATTTAGTTGTAAGAAACGTTACGCTATTCGTTTATGAAAGCATTGCTGTTCTAGCAATATATTCTCTAGCAATTAATAATAATTAACCCAAAAATATAAATTTAATGCATAATTTCTAAACTTAGAAAACTAAAGCAACAAATTTCGCGCCAAAAGACTTAACGGCGATTTATTAAATTAATCAAATCACTACCAACAATCGATAAGAGGTATCGATATACAAAGCTGTTGTGTAAATCGTAATTTATTTAGCGTAAACAGTGTAAATAGCAATATGCGCTATAATCTGTTAGCCAGTTCGTTAAAATTGCTGAATAGCACGAAAACCCACAATTTGTTCTTGGTGAACGTGCGCAGCTTGACCGGCGCCGCCCACACCAACGATGCAGTGGCTCCGACCACGCGCCCACCGCCCGCAGAATCGAGGAATCCCGGAGAGGAGCAACGATCACTGCACATCG
 
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