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CRIMIC CR60255

Gene CG14395

CG14395

Information

Donor	Location	Cytology	FBti	Stock number
pM37_p2	3R :12662831..12673959	87C4-87C5	FBti0219200	Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TGTGGTGCAGTGGAGTTGAAGTTC	TCCGTGAACATCTGCTCGATCT

Images

Construct Design Sequences

sgRNA sequence	TCGAGAACGAAAGTGTTGGGTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right

 

Amplicon 5'

Amplicon 3'

CTCGAATCCCAGTCCGTAGTGCGTGGCTGCAAATAGCAACCAGCATTTTTCCGATACAACAATCGCATTTCATTGGGCAATTTATCAGCCGAAACGGTGTACAAACTAAAAACGTAAAGCTGGAAAACCTCAAAACGCGGATCATATATATATTATTAACAACGATTTTGCAATACCTTTTCCTACTCGTGAATTATGGCCAAATGGTTTTGGGGGCCTGTGTCTTAATCTAATAATGCTAAAGTCTGTCGGTTTTTGGTTTTGCTTGCAGTTAATTAATGAGCCGGTTCCAGTGCGATGATTGAATGATGGCCCCAGTGGAGCACGCCATGCAAATGCAACACCTTCGCCGGCCATCTTACAGTGTGCCTAATATCTCATGCCCAGTGCGATTCGCTAACATGTTGGGAATTAACGTTCATTGCGCCTAAACGGATTGCATTCACCGCTAATTGCTCACAAATTGCTAGCTCCTCCGCCGCCGAAATAAACCAACAAAGAGATCAAGTTAAAAACCAATAATAACCAGTCAGTGAAACAGTGAAAATTTGCATACGCAGCTGAGGCATTCCGCTAGTGTTTAGCACGTCCCACCAGGAAGAAGGAGTAGGAGGATGGTCACCTGCAAGGTGGACTCTCAGACCGCGGTCGCTGTGCCCAAAATGAACTCGGTCCGGACCCGTGGAAAGCCCCCCGCCGCCGGCAGCAACAGCAATAGCAACTCCTCCGGCTTTCGGCTCCTGGACGGCGACCAGCTCGAAAACAATTCGAGTGTCAGCCGCAACTCGATCTACAAACTGAAGATCGATCTCATGTTTGACGATTCCAGGTGAGTTACGGATATGATGTTTTCGGTCATAGTTTCGGGGTGAAATTTGGTAATTGGTAGCAAATTGCCTGGGGAGGTGTGTTTTGGTGGGCCATTAGCCTCGCTCTCCATCTCCAAGCAAAACAAATCAATTAGCCTCTCATTTCCCCAGCTGACCACCC

AACACTTTCGTTCTCGAAACCGCGACTCACCAGATACAGATACAGATACAAAAACTAGTTTCAAGAGATATTTCGTTTACCGTTTGGCATTTCCATAACCAACATAAAACTACAGTTAGTTCCAAAATTAAAAACTATATTGTCCGATGGAGCAGATTCCCAATCCGAAGTGGGGCCATCAAAAGATAGAGCGACACTTGCTCTTTGAATAAACAAATATTCATCGGCGACACACATTCGCCGGCGGACGAACTACTAAATTGATTGAGAAAGAAAACCAAGAGCTCCCCCTCCCCCAGCACCACTCATCTTCATCTTCATTCCCATTCAAAACTCGGAGACGTTCTATGGAAATAAGCATTTCCGATTTTATGTCAGCTAGCGGTAAACGGAATGGCTCGAATTGGGAACACCTTAAGCTGCATTCGGTGTTCGGCACTTGGTCATCGGCCTCCAAGACGCCTCTATCCCGGCAACCTCTCTTTGACCATTTCATGGTTCTCGGGCTCTGACCCGGTCAGCAGGATGTGGCCAATTTGGCTTCCAGCCAACAAGTTCACAAATACCTGACACTCGGACTCAAACTCCGCGGCGCTGCGAATATGCTAATCTCGGCTTGCTTTTGCGTTAATCAGTCAAACTTTCACAAAAATGGAACCGTTTTCTCGATTCAAGCACTAAACGTGTTGACTGGAGAACGGCAATAAAATAAGAAGCGGAATAGATCAACTTTTCAATCTAATCGATGATCTAGATAACATTCTAACTAATGAGTTTTGGGAAAATTAAATTAGGTACCTGTTCATCGCCAAGGATGCTTTTCGGATGGGCTTGGCTATCAAACTAGTTTGATAATATCAATCGAAATTGGGGACTTAAGGGAGTTGTAAATGAATGAAATTCTCGTTGTTAACTTTTGTTAACTTTCATTCATAAAATGAGGCAATCCTTTCTCTCTAACTAGATCCATGCGAAGCAGCCGCCTTGACGATCCCCGTGGATCGCATCGCACCCGCTCCATCATCATGTATGGGCGCAGTGAGT

 

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