CR60256

CRIMIC CR60256

Gene CCT1

CG5374

Information

Donor Location Cytology FBti Stock number
pM37_p1 3R :..FBti0219201 Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK OK GAGTCATCCATGGTGTCGTACACA TCAGATCGCTCTTCTTCACACGAC

Images

Construct Design Sequences

sgRNA sequence AAGATCGCCCTCCCCCGGGTTGG
sgRNA2 sequence
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
 
Amplicon 5' Amplicon 3'
GCTCCAGCCTCAACGAAGTACTTCATGCACAAATCATCAACGCCGCCGCTAACCAGTACTACATTGACACCGGTTCCGAGGATCATGTTGATGCGCTCCTTGGTAATGTCCAGCTCACGGGCACGAATAGCCTCCAGCTTGTCGGGATCATTGATCAAGACCTGGACACCCATCTTCATCTTGGTCTTCTGCAGCGAGAAGTCTAGGCAGGCAATCTTGGCGTTCACAATCTTCTTGGGCATCTGCTGGGAGGCAATTGTGCAGTTCAAGGCATAGCCGGGAATCAGCACCGATTCCCTGGCAGACTTGCCATGGGCCTTCAGGACATTGATGGCTTTAATCGAATAGGCTGCCTGCCCACGGGGATCTGTGATTTTCACCGACTGGGCAGCGTCCACAACCATGGCTGAGAAGAACTCTGCGTCAGCACCAATGATCTTGGAGCTCATCGAGGTCTTGGCAATGTTAATCAGGGAATCGCGGCCCAGCTCATCAACGGGAGCGGTCAGATGCTCCGAGATGTACTTGCAGGCCTCCTTGCAGGCAATGCGGTAGCCGGAGATTATGGAGGTAGGATGAATCTTCTGCTTGACCAGCTCGTCGGCGTTTTTCAGCAGCTCAGCGGCCAGGATAACCACCGAGGTAGTGCCATCGCCGACCTCCTCGTCCTGCAGCTGAGCCAGCTCAACCAGGACCCTTAAAATTTAGAAATGAGTACTTCTTTGAAAGATTTCCCACATCAACAACTCACTTGGCTGCCGGGTGCTCCACCTCCAGCAGTCGCAGAATGGTGGCTCCATCGTTGGTCACTGTGACATCGCCGATGTCGTCCACCAGCATCTTGTCCAGACCCACGGGTCCCAAGGAGCTTTTCACGATGTTCGATATGGACAGAGCGGCCATAACTGAAAGCATATAAAAGCCGAGTTGTAGTCATAACCTCACTTCGCATAGGCTTGTCCACAGGATCTTGTAGCTCCTTGGATTCAATGAGTTTTTTTGTTAATCCAATTAAAATAAGATCGCCCTCCCCCG GGTTGGTTCCGGAACATTCTTCGTGGAGAGCGTGACCCGCGATGAGTAAGAAACGTGCTCCGCCGGCAGACGCACCGCAGATGTTGACAAAACCCCGGGATTCTCACCGTTCTGTGTGCGCACCGATGCGCCGGATTGGCGGGTGCCAGCAATGGACAGAGGAGAGGCCAGGGTCGACATTTTCCTAAGTTCTTCCGCGACTTTTAATAAAAAACAGATAAATCAAATACGCTGAAAAACCGCACGTTAAACCGCCTGACTCACTTTAAGACTTCGAGATGAACAAAAACCCGATGTCGATTCGATACTATCGAGTGCAAGATTTTTTCTTGGAGAGCAAGGATTCCATAACATTTATAAGTTTATACTATGTGTTACATTAGCGCAGCATCAAAAATATATAGAACATATATTGGCTTATTTATTATTAGGCATATTTTGCAGCACATTCAAAGCGGCGTTCAAAGTATCGATGGTAATCGATAACCGATTGTTTAGGGAACCCTGGTTGAGCATCAGGTAACGGTTTTTTAAATATTAATTCAAGATTATTATTAATTATTAATTATTAATTATAAAATTGTGCTTTCTGATGGAGTAAAAGTATTCAGTAGGCATTGACTTTGATTTCGCATTACATATCGTGTGCTCTGTAAGATATTTTCAGTTTACCTCTTGATCTCAACGATATACAGCCCAAATCGAAGCATTTCTTACATCTACAACAAATTGTTAAAGTAAACATTTTTTTTAACTTTTATTTATTACGATAAAGATGTCAGACTGCGGAGCGCGCCCCAAAAAACCCATGAGCGCCTTCATGTTGTGGATGAATTCCACCGGGCGGAAGCACATAAAAGCGGAGCATCCCGATTTTAGTGTCCAAGAAGTGTCTGTGAAGGGCGGAGAGATGTGGCGAGCCATGGCCGATGAGGACAAGATCGTGTGGCAGGAGTCGGCCACCACGGCAATGGCCGAGTACAAGGAGAAG
 
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