CR60257

CRIMIC CR60257

Gene Sb

CG4316

Information

Donor Location Cytology FBti Stock number
pM37_p2 3R :..Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK OK ATTCACATGGCTCCTTTCCGCACT TGGGATTCGAACTAGAGCTGGA

Images

Construct Design Sequences

sgRNA sequence TTAATTAGTGGTGGACTCAGCGG
sgRNA2 sequence
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
 
Amplicon 5' Amplicon 3'
CAGTTATCATCGTTGCATATGACCATCATCATGATGATGGCGATCTTGTGATTACGATCTGTGGCCGGCTAATGCCGCAATCTCGTGTGTGTGGGTTGTCTGGTGGGTGGGCCGTTTGAAGCACCACCAGATCGACCGGCACCACCACATGCATGCCAAACTAAAGTTAGACATCCCGCAGTTAGCCTACTAATTACAGCCGGAGCTCTTTGCGCCCGGCATTCTCGATGCCATTACAAACATAAACCATAGCTTGGCGCAATAAAAAAAGAGCATACGGTTTTCTAACAACAAACTTTGGCTAACCATACTGATTATTACCATTCTCCGTGCAATCCATTGCAGACATGCGCCCGGCACGTTACCGATGAAGCAGCCAACTTTAATCAGACCCAGACTCAGACACAGGTGAGTTGACAACTCGAGGGGTGGTGGGGATGGTTGGATGGGGTATATAAGGGGGCACGTGCAACTGGGGAGGCAGCGATGTGTGCTACCAGTTTAGGTTACAAACCATAAAGGCAAACATCTAATTTGGCCAGCAATGGATTGGCTAAACTTTAATTAAATTTGTTGAAAAGAAAAAAGAGCTATTCCGCTTTGATGAGCTGCAGCGAAGAGGAGTACGAAAGGGGTGGGGCAGCACTGGGGTGCACTTGGTTTTGGGACAAGAAATTATTAATTGTAGCATGTGTCGTTTGACGTCGCCGTCGTGCGATCGCAATAAATTTTTTCCAGTCTTGTTCAACAATAAAACAAGACAAGAGCCACGAAAAAAGCTGATTATGAAGCAAAAGTGAAAAGTTGTAATTAAACCAGTCACTGACTGCACGGCGAGCAGCACAAAAACAAACTGCAGCAACATATGCAGCAGTAGCAGCAGCAACTGCAGCAGCAGCAGCAACAATCATAAAGAGTAGAAATCGTTGCCTGACTTTGGTTTTTTTTTTTGTGCTGCCACTTTTTGTGGCGACAAGTGCGCTTAATTAGTGGTGGACT GGTGGTCCCAAACCCAAACAGGGTTCTGGTGGATGGGTTCCGGCAGGCGAAACAAGACTAATGTCCGAAAACTGTCATGCCCGTTCCTTGCAGAAGAAGCACACCAGCGGCAGCCACCAAGATGTGTCCCAAAAGGCATTGGCTAGTCAACAACAGAGCAGCGGGATCGCGAGGATCAGGAGGAGCTGCGGCGCGGAGCAGGCGCAGTCTAGACCAAATAGTAGAAGTATTAGTAGCCTTAATCGTAGTCAATTGCCTGGCCACAGCAGCAGCGGCGCTGATCACACCGCCCGACAGCCTCGAGTCACTGGGCTCCCTGGGAATCCCGTCATCTTCCGCCTCCTCCTCCGAGGACGACGATGACATGAGTAGCGGCTTCTACCGCATCCCGCACCGCCTGGAGGGCTATCCGCAGTTGCAGCAACTGCAGCGCGGCCAGAACTTCAAGATCAGCCCCAAGCCATGCTCCTTTGGCCGCGTCGAGGGCACCTGCATGTTCGTGTGGGAGTGCATCAAGTCCGAGGGCAAGCACGTGGGCATGTGCGTCGACTCCTTCATGTTCGGCTCCTGCTGCACGCACAACTACACCGACAACATTGTCCTGCCCCAGACGGCCTTCTCCTACACGAGGCCCACCAAGCCGCTCACGCTCCGCCCGCGACCGCCGGCAGCGCCCTACAAGCCGATGATCAGGTAAGAGATTAGCAATTGATGGAATTGTTCTGTTTCGAACGTCAATAAAGATTTAAGATCACTATATCAGTATCACTTCATCAGATTAACAAATAGTGCATATAAAAGCGGCTTGAAAGATAGCATAATATATCTAAATTATTATAATTTTTTAAGTATACGTCTTACTTATTTCCCGATGAATTACCGTCCGCAGCGGCATGACCACCATCGAGAG
 
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