CR60258

CRIMIC CR60258

Gene Amt

CG4316

Information

Donor Location Cytology FBti Stock number
pM37_p2 3R :..FBti0219202 Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK OK GGTGATTGGTGGTAACGGTAGCTT TCCTGGGCTTGAAGTTGATGTC

Images

Construct Design Sequences

sgRNA sequence ACAGGGATAAGAAGTTACGAAGG
sgRNA2 sequence
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
 
Amplicon 5' Amplicon 3'
GATTTATTCCGATGGGCTGGATGCCGGCGATATCTTTCAGATCCACCTTAATTGGCGCCAACGCTGAGATGGCTCGCGATAAACCGATTTGTGAGTGCCGAATCCGATGCTCCGTTAGATCGGCGCCGAGCAGCTCCTCGTGTGGATCCATGCGAATGGGTATAATCTTGTTGATCACATACAGCAGCAGGAATGTGGAGCAGACGCCCCAACAGGCTAAGCATAACGCCGAGAGCGTCTGAATGCCCAGCAGATACCAGCCGCCACCCTTGAACAAACCACTGCGTCCGTTGGTCGTGTCCAGTGGGATGGGATTATCAGCAAACAGTCCCACGGCAATTACGCCCCAAATGCCGCAAACTCCGTGCACAGCACTGGCTCCCACCGGATCATCCACGCCCATACGATCAAAGAGCGGCATGGCCAGGACGCAAAAAAGCGAGCCAATGGCTCCGATTACGAGAGCTTCCCAGGCGCGGTACAGGAAACACCCGGCCGTAATGGACACTAGGGAGCCGAGCACTCCATTGATCAGATCCATGATGTCCATGCCGCCCCCATGGCGCCAAAAGGAATAGCTATGAGGTAACATGAAACCCATATGGCGCTCATTATAAATTCGAATTAACTTACATGGAGCTGGTGAATCCGCCTCCAAATGATCCCATCATGGTCATAACGGCGGCTCGTGCTGCGTACTGCCACTTAGCCCCACTAACGCCATAGGTGCTGCCCGAGTTGAAGGCCAACCAGCCCCACCAGAGGACAAACAGACCCATACACGCGTTGACAGGATTGCCCAGTGGCAGCGGATCATATCCCTCGGAGTAACGACCCAGTCGAGGACCCAACATGGCGGCCGAGGCGAAGGCGGATGCGCCTCCAATCAGATGCACTGGTCCACTGCCGGCAATATCCACGGCGCCCAGCTTGTTGAGGAATCCGTGCTCGCCCCACACCCAACCAGCGGGGATGCAGTACACAGCGGTGTTCAGGAAGGAGAACAGGCAGTATGCCTTAAAGTTGCAGCTGGAAGGATATACAACAAGTTAGTCCCAAAGTGTTTGATACTGTTTGGTGGTAGAACAAGACATGCTTAGGATTTCGTCCTTCG TAACTTCTTATCCCTGTTTGTTGTGCCGCTGTATCTAGTAGAGATGTATCATGGTTACTCACCGTTCAGCCATGGCGCCGCTGACAATGGTTGTGGCCGTGGTGGCGAACGACAGTTGGAACAGGAAGGCGGCAAAAATCTGTCCCATCAGTGCATCGCCCACCGGTGGATCCAGCAGGAAGTCCCCAATGGCGATGAATGGATTGGAGAGTGGGCCGCGACCGAAGCTCATTCCGTAGCCGAAGAGCCAGTAAGTAAATCCGCCCAGAACAATGTCGATCACGTTCTTCATCATGATGTTCACCTCGTTCTTGATGCTTACACAACCGGACTCCAGCATACCGAAACCCGTTTGCATGGTGAAGATGATGAAGGAGGAGGTCAGGACCCAGTTGGTGTCCTCGACGGAGAGGTCATACAGACCGGGAATCACATAGTTGCTGTGATTCCTCGGCGCTCCCACTGTGCCGTTGTTCATGTCCGTTTGGTTGGTCGGGCGATTGAAAACTGCCAAAACGGATAGCAAATCGAGTGGCGAATACAAATACCTAGAACATGAATGTTAAAAATTACCCGCATTTGATTTAAATGCAACTTAATCGTCGCTCAGCAATCGTAGATTATTCACTATGCGTGTTTTTTGCAACGCCACTAATCTGTTGTTGGTAGCTTACGACCCTTAAAGTTTTAAACTTGACACTGGGGTTGCATGCAACGAGTGCATTTTGCAAAATTAATGAGCGTATTTTGAGTGAATTGAATTTAACTATCAGTGGAACTCCGAGTGCGGTTGGAATGCGCCAATAAATTAAAATCTATTTTAAACTCGTGAAGTTTTAGTTGACGTCTTGTCGGTTGTCCGACAAGCATTGCCTATTTTTAAAGATTTTAATATCTCATTCATGAAGGAGGTGACTTTATTGAAATATGTAACAATTTAAGCTAGTATATCTTTAGGCTTTCGTTAGTAGTTTTCCATCGAAGATCACTTTGAGATGTATTATTGCATTAGAAATGAAATTAACATATGTACAAACAGATACAGTGACCCCTAATGCATAGTTTAG
 
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