CR60259

CRIMIC CR60259

Gene crm

CG4316

Information

Donor Location Cytology FBti Stock number
pM37_p1 X :..FBti0219203 Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK OK GAAAGCAGCCAGGAAACGAAAGCT CAACAGTTGTGGTATCACCAGTGG

Images

Construct Design Sequences

sgRNA sequence TGAATTCTAGGAATTAACCGAGG
sgRNA2 sequence
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
 
Amplicon 5' Amplicon 3'
TCGCCCAGCTCATCACCGCTGCTATTCTCCATCTTCATCAGATTGCCCTCCAAGGGCCCGTCGTCCAAACTCTTCTCCAGCGGCGGGCTGGCACTCGATTTGGGTTTCTTTGTCTCCGGCGAACGCTTCTCGGAGCCGCTTTCCGTTCGTAGTCGCTTGTTGGCCGCCACCGACATGCCGGCCAAATTGCCCAAGGTCTCGCTGCGCACTTTGAAGCCCATGCGCTCCTCGTATGCCGTCAAGCAAATACTGATGCTGCTTAGGTAGGCGGTGATGCTGAGCAAAGGTCGGTGGATGGCCACTCCCGGCCGGGGCTGGAAGCACATTGAGGGATCCAGCGATGCAACTGACGGCTGTAATGGTTCCGTTTCATTGTTGTTGTTATTGTTGTTGGACGCAGCAGATGGCAGGCTGCTGGAAAAGTATGCACTCTTCTCCTCGTGCAAACGCTGATTGGCGCTGCGCCATTTGTACTCGAACGTCTTGATGAAGCTGATTAGTTTCTTGTGCAGCGGAACTATGATCCTGCCGCGTGGATTCTGCGCCAGTGACTGGACGCGTCCGAAGGCCTCCATATTGGCGGGAGTCACCAGCACCTCCACCTTGCTGGGCAGCCGGATATCCTCCAGTGAGTCTACGGTTTGTCATTGGGTATTATTGGGCAAACATATCGAATGGAAGCGGACTCACCATCCAATTGATTTAGCCGCCGGAGCGCCTTGCACGATGGCGTCTTAATGCGAATGTTCTTGCCCTTGCAACGCACGGTTATTTGGCCCTGGTAGACCAGCTGCTTCAGCTTCATGAAGTGCTTCTCCGTGAGGAACTGCAGCTTGCGCCGCATCTCGCCGTAGTTGATCAGCGTGTACAACTCCTGGG CCGAGGCACGAGGTTTTTGGCGCCGAAAATCTATTTTTCCGGGAAACCGAAACAAGCTAACTGTGACCTTATTAACGCATTATTTTGCCAATAGCTTGTTAATTTAACATTTCGGGACATGTCATATCCAGTTTGACCCTTCTTCTTACCCTCCGAGAAGCGCACATATTTGCAAATCTTGTGATGGGTCTGGTAATAGTGCTGGCGAACTTGGTCCTTGGTTTTGAAACTATAGTCGCTGTTGGCATTGCGGCGCTTCAGCTTGGCATTGATGCAGTTGGCCACCGCCTCGAAGTCCTTGCCGAATTCGTTGAGGGCGTCGAAGAAGCAGTTGCGCTCCACATTCGTCCAGGTGGTCTTCCCGCTGCCCGTCTTCAGCTGGCTGGGCGTCTTTTGGGCGGCCTTCTCCTCCTTTTTGTTCGGCTCCCGTTCGCTGGGCGGCGGTGTCATCGGGCGCGTCTGGTCCTGCTTCATCTTCTGTATGACCCGGGCACTGGCCCGCGTTCCCGGACAATTGTGCATGGTCACCGAGCCGAGGAGCTCCTCCTCGGGCAGCTTAGCTATCGAGTTAACAACTACTGCTCCTCCTCCCTTGCCATTTGGCAGCGGCTCCTCAATGGACACGGATGACGACGGCGGCGGCGGCTGCGTCAGCGGGGGTGGCGGCGGCTGCTTGGACAGTTCCTCCATGTTGGCCAATCCACATCCGCTTGCCTCCTAGACAGGATCAATCAATGGCAATATCAATTAAACTGCGTAGTCTTCCGGGAAAAGTGGGAAAATGGCGCCGAACCGTCCGATTGCTTGGTATTGTCGCTGAACCGCGCGCCCGGGCTAATCCACTACCGACCAGAAATGATCTCGAAGGCTTTCCCAAGCTCCGACGTGCACTCACCTACAAATCAGACATGGAAAATCGGTGCCAAACGACTATATAAAAAACTTATTAACGAGACGCCAGGGATGTTGATGTTGGCCAACAGTGTGGTATCAACAGTGCAGTGTGTGCTGTTAGGCGCGAAGTGGTTGCTGTTAAG
 
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