CR60260

CRIMIC CR60260

Gene Dip-B

CG9285

Information

Donor Location Cytology FBti Stock number
pM37_p2 3R:..FBti0219204 Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK OK CAACGCTGAGGATGAGTTGCTTTC ACCTTGAGAACCTTCTCCATGGAC

Images

Construct Design Sequences

sgRNA sequence TCGTTTTGGAATTATGGGAAAGG
sgRNA2 sequence
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
 
Amplicon 5' Amplicon 3'
GGAAGGCGATGCCGTGATGACGCCCTACAGGAAACTCCTGGTCCTGGAGGCCAAAAAACTTCCGCAGCTTATTGACGATATACAACGCTTTATTAATGGCGAATAAATATTTTTTACATTTAAAACTGCTGTGTTATTTGGCCTTAATATAACACCCCGACAAATATCTAATTAAGAAGTAATAATGTCCGGTCTCTAACGACCGATAAGCTTCTATTTCTTTGTAGTCCCATACAAAGAGTATCAAAAATTCGAACCGAAAGCTTTCCACATCCTTTATGACAATTGGCAACGCGGGCAAACCACAAACAGCTGTCACTTGGCCGGCATTTGAAAGCTTCGCACATGCCGGCTGGTCTACTGTGAAAAAAAAACCTGGTTGCCATTCCTTTGTTTTTTGCCACTGGTTAGTTTTGGCCCAGAAACTGCATCCACCGTGGACGTGACTGGCAAGAGGAACCCACAATAACAATTCCGAAGACCGAGCGACGTGAGTAGACTTTGCCTTTATTGTTTACAAGTTATATCCGAGTTCTAGAATTACGTACATAGCGAGCATACTGGCGATTATTCATCGCTCGTGCGATTTGTTTTCATTCGAAAACGCAAGTAGCAATTAAAAAAAAAAAACCTAAAGCCCGGCTCGCTAGTAAAATGTATAAAAAGCTTGGCCACCCAGCCAGAACATTGCAAATCGTCGCGGAGAGCTATCACAATCGCTCCTTATCGCCAGCTTTCCGTTCCAGATCCCGCTCCCAAGTTTCCAGTTTCCAGCTGCAGCGCTTTGTTCCCATCAGGGAGGTTCAGTGACGCCCCACTTGCCGATCAACCAAGTCGCGTGAGCTGCCTGCGAAAAAGTACTCATCATGGGTATCGAAAAGTGAGTATTTATTACCAACAGTTATTAGAGGGTGTGTTTTAAGTTAGCGCGGATCAGTTATCTAGCCACCGCTTAGCGACCGATTGGAAAAGCTCTCAAAGATTCGCCATCTTTTACAGTGAGGGTTAGTCGCTTAGCATCCATCAATTGC GTTGCCGGCAAGGAATAGATGTCATGAAAATTCCAAACTTTATCTTTTCCCAAAATAAAAACAATGTTTGGCGAAATACTGTATAAAAGTTAAAGGGGGGAGGTCACTTAATTTTATCATTATTACTTAGATAACGAGTTCTTGCTACTTCTGGAAACAGATATATATAGATCAACTTTTATTGTTTGATATGGGGCACTTCAAAAGATAACACAATTAAATGGTCAGCGACATAGGATTATTCTCCAAAATATTATAGACCCAGTTACCTAAGGATTGGTATTTAGTAGAAACACTTGAATAAGATTACAAATATTCTAAATAAGTACTTTAACACCATTTGTGCTAATACTTGGTACACTTGTTTCTAAATTGGAAATCGGTTTGTGATTATGGTCATTTCGAAAGCTTATCGCGATTCTTTATAGCACTTCGAGTTAAGATAAGCCCAGCGCCAGGAATGAGGGTAAACGAAAGGGGAACACACTGAAACATGGCCATGGTTGCTCCTAATACAGTGGGGACATTTTCTTTTGAAATAATAAATTGAAATACAAAGGCAACAGGCTGTTATGCAATATGAACGACTGCATCAGTTAATAAAGCAAATGTGACGTGATAAAATTCAATTCAATTAAACGCTATTGCACATTTAGTTGACACCCAGAAATGTTTCTTAATGAATACCAATAACGATTCCGTTGCGCACTTTAACTCTACTCTACGTGTGCCCCTCCAGAATTCTACCATGCACCCTCCGGCTGAACAAGCTGATGAGTCTGGCCGGCAACGACTGCCTGTGCATCATCGATCGCAATGCGGTTCCCAATGAACTGAAGGCCACCTTCGAGGAGCACCGCAAATTCGACAAGTCCTTCGACAGCAGCATATCCTGCTTTAAGGTGCCCAACGTGGATCAACCTGTGGTCTATGCCCCCGTCTCCGAGCTCACCGACTACGAC
 
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