| ( ! ) Warning: Trying to access array offset on null in C:\wamp64\www\pscreen\crimic\info.php on line 28 |
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| Call Stack |
|---|
| # | Time | Memory | Function | Location |
|---|
| 1 | 0.0001 | 358192 | {main}( ) | ...\info.php:0 |
| ( ! ) Warning: Trying to access array offset on null in C:\wamp64\www\pscreen\crimic\info.php on line 29 |
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| Call Stack |
|---|
| # | Time | Memory | Function | Location |
|---|
| 1 | 0.0001 | 358192 | {main}( ) | ...\info.php:0 |
| ( ! ) Warning: Trying to access array offset on null in C:\wamp64\www\pscreen\crimic\info.php on line 30 |
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| Call Stack |
|---|
| # | Time | Memory | Function | Location |
|---|
| 1 | 0.0001 | 358192 | {main}( ) | ...\info.php:0 |
| ( ! ) Warning: Trying to access array offset on null in C:\wamp64\www\pscreen\crimic\info.php on line 31 |
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| Call Stack |
|---|
| # | Time | Memory | Function | Location |
|---|
| 1 | 0.0001 | 358192 | {main}( ) | ...\info.php:0 |
| ( ! ) Warning: Trying to access array offset on null in C:\wamp64\www\pscreen\crimic\info.php on line 32 |
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| Call Stack |
|---|
| # | Time | Memory | Function | Location |
|---|
| 1 | 0.0001 | 358192 | {main}( ) | ...\info.php:0 |
| ( ! ) Warning: Trying to access array offset on null in C:\wamp64\www\pscreen\crimic\info.php on line 33 |
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| Call Stack |
|---|
| # | Time | Memory | Function | Location |
|---|
| 1 | 0.0001 | 358192 | {main}( ) | ...\info.php:0 |
| ( ! ) Warning: Trying to access array offset on null in C:\wamp64\www\pscreen\crimic\info.php on line 34 |
|---|
| Call Stack |
|---|
| # | Time | Memory | Function | Location |
|---|
| 1 | 0.0001 | 358192 | {main}( ) | ...\info.php:0 |
| ( ! ) Warning: Trying to access array offset on null in C:\wamp64\www\pscreen\crimic\info.php on line 35 |
|---|
| Call Stack |
|---|
| # | Time | Memory | Function | Location |
|---|
| 1 | 0.0001 | 358192 | {main}( ) | ...\info.php:0 |
| ( ! ) Warning: Trying to access array offset on null in C:\wamp64\www\pscreen\crimic\info.php on line 36 |
|---|
| Call Stack |
|---|
| # | Time | Memory | Function | Location |
|---|
| 1 | 0.0001 | 358192 | {main}( ) | ...\info.php:0 |
| ( ! ) Warning: Trying to access array offset on null in C:\wamp64\www\pscreen\crimic\info.php on line 37 |
|---|
| Call Stack |
|---|
| # | Time | Memory | Function | Location |
|---|
| 1 | 0.0001 | 358192 | {main}( ) | ...\info.php:0 |
| ( ! ) Warning: Trying to access array offset on null in C:\wamp64\www\pscreen\crimic\info.php on line 38 |
|---|
| Call Stack |
|---|
| # | Time | Memory | Function | Location |
|---|
| 1 | 0.0001 | 358192 | {main}( ) | ...\info.php:0 |
| ( ! ) Warning: Trying to access array offset on null in C:\wamp64\www\pscreen\crimic\info.php on line 39 |
|---|
| Call Stack |
|---|
| # | Time | Memory | Function | Location |
|---|
| 1 | 0.0001 | 358192 | {main}( ) | ...\info.php:0 |
| ( ! ) Warning: Trying to access array offset on null in C:\wamp64\www\pscreen\crimic\info.php on line 40 |
|---|
| Call Stack |
|---|
| # | Time | Memory | Function | Location |
|---|
| 1 | 0.0001 | 358192 | {main}( ) | ...\info.php:0 |
| ( ! ) Warning: Trying to access array offset on null in C:\wamp64\www\pscreen\crimic\info.php on line 41 |
|---|
| Call Stack |
|---|
| # | Time | Memory | Function | Location |
|---|
| 1 | 0.0001 | 358192 | {main}( ) | ...\info.php:0 |
| ( ! ) Warning: Trying to access array offset on null in C:\wamp64\www\pscreen\crimic\info.php on line 44 |
|---|
| Call Stack |
|---|
| # | Time | Memory | Function | Location |
|---|
| 1 | 0.0001 | 358192 | {main}( ) | ...\info.php:0 |
CR60261
CRIMIC CR60261
CG7780
Information
| Donor |
Location |
Cytology |
FBti |
Stock number |
| pM37_p2
| :.. | | | Order from Bellen Lab |
PCR Verification
PCR check relative to sgRNA sequence
| HA-L |
HA-R |
Forward |
Reverse |
|
|
GACATAACAAATCGGTACACCTAAGT |
ATCGCAGCTATTCGGTAAGTTGCC |
Images
Construct Design Sequences
| sgRNA sequence |
TAAATGAAAAAGTATGTGAAAGG
|
| sgRNA2 sequence |
|
| Amplicon PCR 5' Left |
Amplicon PCR 5' Right |
Amplicon PCR 3' Left |
Amplicon PCR 3' Right |
|
|
|
|
| Amplicon 5' |
Amplicon 3' |
| ACAGCCACCAGCGATAAGAACTAACGAAGTTGAATTCAATTAATTTACCAGACAGTTTATTTCCAATATAATTCAATATTAGCAGATGGGCTCGCCCGCCCGCAAGACAAATTATACAATTTGATAACGATAATATTTTCATGCGATCACGATCGAGGAGGAAAGCACAAACGATATGCCCTGCAATGCAAACGGGGAAAAGAGCAACAAATTGCAGTTTTCAGCAGGCGACAAATTATAATAATACAATACGAATAATAAATAAAAACTTTAACAATTGTTTTGTGTAATTGTAAAGAAGTGGAGTTCTTTTCACCGCCAGTGGGATGAATGGGATGTTTATGTGATGATGGTGAAATGGGTAAGTATTTATAATATGTTACAGATTGGACAAGATTCTTAGTAGTTTCTTATAATATCCATAAAAATGAAGTCAAACATATTTAGTAGAGTATAATATATTCGTTGTAGCCGCATTTAGTGCGCTTGGCTTATCAGCGACCTCTCTGTTCGACTGATCTTTTGCAATAAGTGGCTGACCCCTCAGATAGCAGACAACGTGGCGATAGGCTGTGTTAAAAACCCAAACACGACCAAATATTTACAAATCCAACTTTTAGATAAAACAGACAAACAGCAAGGATCTCAGTTTTCACTCGATTCAAGCGTTTTTACCTGGTGAAGAAATCGAAAAAGATTAAGATGGATTTCAATTTGCGACTGAGACACCCTGTATGAGCACAGCCGTTGATTGGATTGGATTCACTGACACTTATTTAGTTGTTTTCCGGCCGAGCAACTTGAAGGTTGTACAATGCGATCTCTGTGCTTCGTTTTGCTGTTCGTTTGGTTTTTCTACCAAAACGAAGCTAAGTCTAAGGTTTCCTGTAAGGATGAAGCTGGAAACGATGTAGATTGGTAAGTATTTCGATTAACAGATC
|
CTGCAGTCGCTGTTTGCATATCATTAACAATTTGCAAACACAACGTATTTTAGTTATTTATATACACTGCTGTATTGAGATTATTCTAAAAGAGGCGATAAACAAATTGAATATGATGAGTTCAAGTTCAAGCGGAAAAATTGATAATAATTGTACAATACTGTGTGACAAGAAATTAAAAAGTAATTAGTGTGTTATTTTGTTTCCTAAAATGAGAGATTTAAATCAAAGTTGTTTGCTACAAACAAACTTAATCAATTTGATAACAATAACATCATTTTTAATACTTTTCCATTTCCAAAAATAGATTAAATGGTAATTATTTTTTTTTTTTAATATCTTAAAAACAAATTTCCAAAGATTGAGGGGTTTTCTCGATGAGATAAACATGTTTTTAATTTATTTAATAATTTTTTTTCAAATATTCCTTTATAATTTTAGGTGGCACCTTTACAAACTGCCCAAGCACTACCAGCACAACGATTTGGGCAAGGACACCAGTGGCTTGAAGTACCTGTACGTGACCAGCCAGAACTATGACACCTGGCAGATGTCGGGAAAGTTCATTAGTGATCCCCTATCGCTGCCTGCCCAGACCCTTAACCCCTTGAACGACGATCCCAGCCACACTCTGCTGGCCGCCTACAACGATCAACAGCCGAATGGCACGGTTTTCAGCAGCGGTGGTCATGCCAAAGGAGTGGTGGCCAGTGATGGGGAGACGGCTATTTGGATTGTCCACTCGGTGCCCAAATTTCCAACAATTCCGGACTACAGCTATCCCACATCCGGCGAGCAGTACGCACAGAGCATGCTCTGCGTCACACTGAAGGGCGAGGATTTGGAGAAGGTTGGTCAGATTCTGGTGTACAATGAACCGCACTTCTACTATCAACGCAACCCGCTGGCCACTCGCTCCGATGAGCTGTTCCCGAGCCTGGAAAGGGCTCTGCATGGTCAGTGGCGGACGGAATCACCGTTCCAGAAGGACTTGGAGGTGCGCAGCTTGGACGGCAAGAAGTTCC
|
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