CR60262

CRIMIC CR60262

Gene apn

CG15887

Information

Donor Location Cytology FBti Stock number
pM37_p1 3R:..FBti0219205 Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK OK AGAGGCACCCAGATCATCTAAAGG GAGCTATGAGCAAAGTCACGGAGA

Images

Construct Design Sequences

sgRNA sequence ACGTTTGGCTAATATGTGTGAGG
sgRNA2 sequence
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
 
Amplicon 5' Amplicon 3'
GGGCCCCAGACAATTATCGGCATTGCTGTTCCGACTAACTGTGACCCATTTCGATTATTTTGGAACTGGGAGTACGAGTATCTATATCCATATATAGCTTTCTATACGTGTGGCACTGCGGAAGAGCAGTTAAAAGAACCGTAAATCACGAACGCCCTCTGTTGCATACAAAATGAGATAGAAATTTCACTTTTCATTTCAGCAGTAAGGCACACGCAATCGAAAAAAATCCATTGGCGCTGTCAACAGGAAATTTCGATTATTGATTTGTTACTTAAACATCTGATAAACATTTAGATAAGCTCCGACCAAATAATTTACACTTTTAATATTTTCATTGAACAAGGTTTTAGATGTTTAAATGTGCTTTTATTATAGGTATTTTTTTTTTTTCATTAGAGCTTTTCGTTTCCCAAATTCTTTATAATATTTTAACATGTTTTGTCTTCATTCAGCAGACTAATTTGTATAATAATTTCATAGTACAAATTCTCTGCTAAATGTCTTTCTCTTTGCCATCCTTGACTTGTAATAAATAAATAAGTTAAATAAATAATTTAAACTATACTTAAATTATTATTTGGTTGAACGCTCGCTGAGGCTGAGGTAAATCTGAATCCTGACTCGCTACCAGCTCGCCGTTATCCCTGGTCTTGCATTGTCATCCTGGTCAGCCAAAACCGAAACAAATCATCTACCACTCCTTCTCCAACCAGACGGGCTGCTGGGTGTGCGAGTGGGAGTAGACCACCGGCACGGGTGCCTGGACGGGAACGGGTGCTGCGGCATAGGCGGCGGCTGCCTGGACGGGAAGAGCACGGGACAGCATTTGTCCGATGGCCAGCACCAGCAGGATGACACCCACGCCCAGTCCCTTGATGGAGACGACGGTCAAGGCCGCCAGCAGGGTGGTGATCACGCCCACGACAAAGGCGCCGGGCACCAAGGCGAAGCTGATGCGCCGCAGGAAATGATGGCCAAAAGTACGACTTTCTGGAATAATGAAAAACACACAAATTTATTTACCACAATATCAAAAAAATATAGAAAATTTAAGTTCTTCAAATGTAAGCAGAACGTTTGGCTAATATGT CGCCACATCCGAATCCGAATCCGAGCTGTTGGCCGCCTGCTGCTGACCCAGCACCAAATCACATGCCAAAAAGAGGCAGACGAGGGCGAACACAATCTTCTGGTTGGCGGCCATTTCGATTTGATTTGACTGTTGGTGATTTGATTCCTTGAGGATGAGTATCTGGTAATCCGTTCGACACTGCACTGCTCGACTCCGACGCTGGCTAACAATTGAATGGAGGGTTCACATTGACATTGGCTTTTATCGAGGGTCTGGGCCGGCTTACTGGGCGGCTACTTGAAATTCGGGAGCCGGACATCGCGTAGCCGCAGCGTAGCACGAACGTGCGCCAAAACAAGCAAATCGTGTCGAAGCCATACGACTCGTACTGAAATTACACTGTACCGTCCCATTGATGCCGGCACACTGGAAAAAAAGTTTATTTTAACCAACAAAGATTCCGATGAAAGTATGATGCTGCATCAATAAAATCATTTTTACACAGTTCTATGATTTGAATATGCTTATGCTATTATGCTTTTTAACTTGTCACAATTTGGCTGATTAATACATGGCAAGTGGATAACTTCCTATTTCTTGCAGTCTATATTTTTGAATATGCATGCAGTCAGTCATTATTTTCACTACCTCTAAGGGCCCTTTTTCGGGCCCAACGCATTTGGGGGTGTGCTGCATAGGAACTTGGACATGGAATGGAACTTGGACATGGGCATTCTTTCACGACGCGTGTCTATATTGAGGGCAGCACCCAGGTCTTCGCATTCGATTCGCTTTCATTTTTCGTCGCGGGCCGATAATGATTTATCAGCTAATTAACAATGTCGTACTGCATTTTAATTTATAATCTATACATGCGGCTTAAACTGCAACTATGTTTAGCCATTCGACTGAGTAACGGATGTTCATTGATCAATGCTTGGCATTCCACGCCCTCGGCATCGCAATTCACTTTCATTAACGGATATTGTATATATGAATATGCAAATCACATGTGAGTAATGCGTAC
 
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