CR60263

CRIMIC CR60263

Gene CG8483

CG8483

Information

Donor Location Cytology FBti Stock number
pM37_p1 3R:..FBti0219206 Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK OK AAAGGCTCTGCTCTGCGATTCT CAGGCGTTGACTTCACCTTGAACA

Images

Construct Design Sequences

sgRNA sequence ATTTCAGGGCATGTCTAGCATGG
sgRNA2 sequence
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
 
Amplicon 5' Amplicon 3'
GCTCAGTGGCAAGGACTACAAGACGTTGAAAAATAACCGACGCATTTAGCGCGAGTTAATTTTCATAGTTATGCTTTATGTTTCGTTTCATTTTCATTTATCATTTACTGATTATCACTACAACTAGCCTTGTGCCCAACATATTTAATTACATTATTAGCACGACGGTCTTTTGATTGATGCCGCAGCAAAATGATGCGGCTTAACAGCCATGATACCTGTGACATGCGATTAAATTGGAGTGCCGTCCCCTCCGAAGGAAACACACCATTTAATCGAGCTGATATCGAAGACAAGGCATAAAAGCAATCATCAATCAGCAACGCACACGCGCACTATAAAAATCTTTTTGTTTTCATTTCGCTTTTTTATGGCAAATTGGGATTTTTTGGGTGTTTAGCGCCGCGTGTGCAGAACAAAGAACATTAAAGCTTTCGGTATTTCTATTTCTAACCGAATCTCATAAGCGTGGCCAAGATCCGCTCAATGACCCATTAAAAATCGAGTGATTTGCCAGCGCATTTGTGACGCCTGGTAACTTAATTTGTTTACAAATTTCATCTTGGCGCCTCGGTGGGGCAATTAAAAAGTCAAACTCAGTTCAATGAGCTCCTTTTGCACGCCTGATTATTAGGCGCCACTAATCGAGCTCAAGGGTTCTACGAGGCTCCTGCTCTGATCTCATTGAATTTTAAGGGGATTTAAGGGGATACAGCCTTTTGGCTGTATGAGCGGCTGACAGTTGATAGGTCAGCCACCTAAAATAATTTTAATTCGTTTGGGCACTGGCAAATATTATATAAATTATATAAACCAGATATTTTTTAACCGGTTCCCATCCCGCTCGACGCTTAAGTAATATTTCGCCTGTATTTGTTGCTTTTCTAAACTATTTATTATGCAATATACACACTGTTTAAAACTGGAAAATTAAGAAAGGTGACTCGGATGTGCCAATCCGAGTCTATTTTTGGTGGGACCGGCCAAACTGGCAAATTAAGAGGCATTTACATATTGTTCAATATATTCTCGATCTACCCTCGCTGAAATAAGCAGATCGCAGC GGGACCTTGGTGTGATTTTCTTGAATAACTTACCAATTATCTTTCCATTGCAGGCGAATGCCACTTCGCAGGAGATGATCATAATTGTGGTTAGTAATACCGCACTTGGCTGCATCATTTTGGTTATATAAAATTGGGTTTTAAACAACACGTTGATTTCTTCTTTTTTTTTATTGATTTAAGCACTTTGTTAATTTTTCAAATATAACCTTTTCATAAATGACAGAGTTATATCTGGTATTCCATACACTATAGATTTGCAATTTTGCTAGGATTTTTTGTATCACAATTTTGAACGGTGGGCGTGGTGGATGAAATGTGGAAAACCGAAAAAAAGAGCGCCACGAAAAAAAGCCGACGACTCCAACTCCACTCTCGTGTACGTGCAGCTAGACTTGAAGGCGTCGCCTGTTTGATTAACCGCTCGGCGGCGCCAACGTTTTTATACCGCCGATCGCCGGCAGCGGCAGCGGCAGAAGCGGCAGCAACAGGTAAAGAAGCTACCGATCGCCCAGCGGAATCCGAATCGGAATCCCCAGGCCAGAAATGTGAGCAAAGCGAGAGAGCGACGCAAATAACGCTGCGCAGTCGCAGTTACGCGCTAGCAACTTGTTGCCAACGCGCCAGCAACATGCTGCTTCGGGTACGGCTGTTAGTAAGATACAATGCTCAGTTTTGTGTTTTTTTAGCCGGCGTAATTGAAGATTGAACTTTATATTAATCATCCTGCTGGTTCGGCAACAATCACTCGCCTTCGCCGGGGGCGTGGCAGCTGGCCGGGTCGCTGGGAAAACTGCGAAACCCGCGAAATCGACGAGCAGGTGCCCTGCGGGCAGGCAGTCGAAGCTTCGATCATTTCGCGTATCATTAAAAACCAATAAAAACACCCAATTAAATGCACAGTCGCCGCGCACAAGAACAAATGCCACTTGATCCTTTCACCATCATCGCTAGTGGGGCAGTCATGTATGTATGTGCGAATATCTGTATCTGTGTCTGCCCATTGAACTGACCCTGTTCGCTTTTGCTCCGCGCATTTC
 
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