CR60265

CRIMIC CR60265

Gene CG2519

CG2519

Information

Donor Location Cytology FBti Stock number
pM37_p2 3R:..FBti0219207 Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK OK GGACACAATACTAGAGTGAAAGGCGG TGGAAGTGCTTTGCGCATGATGTC

Images

Construct Design Sequences

sgRNA sequence CTAAACAGATTTGAATGCAGTGG
sgRNA2 sequence
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
 
Amplicon 5' Amplicon 3'
CTGTGCCAGCCACTTTGTCGGTCAGCGCTTCCGGCACTCAGATGCTGCGCCCTCAGACTCTGGCCGGCCCCGAGCGGGAGCCTATCGAGTTCAACATCAACCTGGTGGGAGCTCCTCCCGAGGTGGAGCAGCTGGTGGAGCAGATCAAGAGCGTGGCCGAGCAATTCCTCTACCACTGGAAGAGCTTTCCCATTGGTGAGTTGACATGCACATTGCGGTGATCCACGACACTAACCGAGCTCTATTTCAGTTTTGCCTCAGGCGCTGGCTAACAGTGGTCTGAATTTGACGGCGAGAAACGCGACCAATAGCGTGAGCAGCCGTAAAACACGTCCCATTAATCTGCGCGACCTCTTTATAGCGCCTCCCTTTGACGAGCTGGACGCCGTGGCATCTGACGGGAGCGGAGAGCCCCGCCGGCTGACGAGCGCCCAGCTGAAGACTCTGCGGGAAACTGGGTGAGTTTGCGGTCACGAGACAGCGTCGTTCATTGCAATGATGGATAGATTGATGGATGACGGATGATGGATGGAGAGACGCGCAGTTGCTGGCTAAATATGCAAAAGGGGCAATTGTGCAGCTAGCAGTTACCAACTTGCTGATTAACCGCGCTTTGGCCACATCCCCCACACCTGATAGTGATTTCTTTCGTTATTTTGAGTTCCCTAACCATTGCCAATCATTCCGCGACACTTTGCACACAGCAAGCGCAAGAATCAAATTAAGGCATGATCCAAATCTATCTCAAAATGAATACGATACCAGCTTTCGTGGTCTGTTTATTGCTATTTGTTTTCTAACTAAACTAAATGCATGTCCCACTACAATTATGTTTTTATTTCTGTGTATCACTTGCCAATGAATCATGAAACGGTAAACGAAAATTTGTGTGACATATTTCTGTAAAGCTTGCAAAAGGATAAATATGGAAAGCCGAAGAAATTAACTTTGGAACAATTGGAAACAATACGTAAAAATGGGTAAATAAACACTATCAAGAAGGCATATACAAAAGCCGAGGCAAACTTCTGCCACTG TCGCTCTGATAAATTTGCATGCTCGTTAAACTGAAAATAAAGCCCAAAAAAGAATGTGCATGTTCCGAAACTGTGGACCGCACAAAATACTTGGGGGAAAAGAAGACAATCCGAGAAAAATGTCATTGTTGCACATTTGGTTTGTAATCTCACCAAAATTTTACTTAAAAATCTCCGACGTGCTGACGACTATAGCCATTCTACGAAACGAGTTCTTTGCAAGAACAAACATCCATACCCCAACGCACACTCACAATCTCACACAAACATTAAAACATATAATATAATTCACCTATAATCGGGCAAACCATAATTCTGAATCTGGTACACGCTTGTGCATGCCGACAATTTGTCATTAATCTAAAATCTAGATCATTTAAGTGTTTTATGTTGCTCTGATCTACCAGTCGTGTCGAATAGCTAATTCGTTTCCCCACTCATCCACAGCGAGTTCGATGTGCCCAGTCTTTATTTTCCTGGCCAGGTGCACAAGTGGCGTCTGTCCCAAGTACTCCAAAAGGGCAAGCTGCGGGCGCAGGACTCCTTTCTGAGCGATCTGGCCTTGGCTGCCCGGTTCGTGGTGGTCACTGCCCGAGCTCGCATCTTCTCCCACTTCTTTTCGGTCGTGCAGGCGATGCACGGCTTGCTGGAAGCGTTCACCAGACTTGCTGATATGGTCATCGGTGTGCCGGCTCTTTTGGCCCACAACCTGGACTATAAGATTAAGGAGGAGCGCTGCCGATTTCTCATAGCCGAGTTGGTTTGTCGGGTAAGAAATATTTACCTAGAATTCATATTTCACATTGGCCATTATATACCAATACTATTGATTTGATTTAAATACATCCTTATATTCGTCTGTCTTATACGTATTCTCTACTTAAATTTGTTTTAAATCAATAAATAAATGCATATTCTATTAAAATATGTTTTTGTTTAGCCCGAATACGAGGATTGTTTACACGGCCTGTGTTCCTATGTCCGGAAGATGCTGCGTCGCGCTACGATGGAAAAATTCGACTTCAACAGCTGCGAGGTCAGCCAGCCCGTACCCTATCTATTCC
 
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