CR60267

CRIMIC CR60267

Gene Osi1

CG7780

Information

Donor Location Cytology FBti Stock number
pM37_p1 3R:..FBti0219209 Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK OK TCTGATTGGCATCGTTGGCTCGAA GTCTTTCCACTCGGAACCTCTGTT

Images

Construct Design Sequences

sgRNA sequence AGCAAATGGATGACACGCTGCGG
sgRNA2 sequence
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
 
Amplicon 5' Amplicon 3'
GAGTACGGATCGCTATTCACTTTCGATGGCCGGCTTTCATTGGGACGAACACTCACACGCAGCGGGAAGGCGGTCAATTGGCGGTTGCCTGGCTTCATAATCCCGCGACAATGCGATTCAATTTGTTGTCGATCGAACGCTGATCACCGATCGCAGATCGCTAACCCAGAAACTTTCAGTGGTGGTGGCAGTGGCATTGGCATTGGCATTGGCGGCAGGAATTGCGATTCCGGTGCATGCCCCAAGCTCATACTCCATGCTCCACACTCCATGTTCCATGCTCCATGCTCCATCTACATGAGCACTGGCTGCGAAAACCAGTAGAGTTGGGTAGTGGTTGCGGCTGCGCGATAAAAGGAGCACCAAGCACCTCTCGGTTGCCATTTGCGAGTCGTCCATTCAGCCGTGCGGTAGTGTCCCAGTGATCTTGCAATCATCCCGCGTTATATCGATCAGCAATCCAAGTTTCGACCACAAACATGCTGCTGTGTCGCGTTGTGCTGGCACTGCTGCTGCTGGTGCTACTCCACTACTGCCAGGCGAAGGATGAAGGTGAAACCACACAGCGTGGAGCAGTGCTGGTCTCACTGACTGAGAACATGGCCAAATCGGCAGGATCGCAGCTCGTCCAAGCCGATCCCTTCATCTCGCGCAACCAGAAGCAGTGCTTTGAGACCCGCAGTCTGGTGTCCTGCATCAAGTACAAGACCAGCAAATTGATCTGGAAACTGGCCACTAACAGCATGGGCTTCTTCCCCAGCGAGTACGGGCGCGATCTGGCCGGGGACAGAGGCCGCTGGCTGAGATTAGTCCAGTTGGGCGAGCCCGCCGATGAGGTGGTCGTGTTCAACGACGCTAAGAGCTTAGAAGGTGGGTCTGCGCAATCGAAAGTTGTTTTCGAGAAAATCTCTAGCTTGAATTAATTGATCAACTGCGCCTGGTCAGTATCCACAGCAAATGGATGACACG CTGCGGATGTTTACGATCCATCCGGTTGTAAATTAATTTGAAGAATTATAGCCTCTGGTCTTGCATTTCTCGTGGCTCTAATTGACTTTGTTTGTTTTGAAGGTGACAGTGAACTGACGATGATTCTCAAGTTCCTGAAAAGGGCAATGGAGACGTTTGGGCGAAACCACGGACTCCAACTGAGGCTGAACAGCGAGGGTGGAGCGAGAGTCATGGAGGAATCGGGTAGGTGTTTAAGAAAGCTACCATTAAAAATTGAAATATAATGCACATACACCCTCCTTAGAAGCACGATTGAAGCGCAAGAAGAAGAAGTGGCTCATTATCCTGCCCCTCGTCATTCTGATGAAGATTGCCCACCTGAAGATGACTTTGGTGAGCATGCTGATGGGCGTGCTCGGCATGAACGTGCTGCTGGTCGGCGGCGTGGGCTGGCTGATCCACTACCTTAAGTACAAGACCATGTGCAAGATCCACCCGCATCTGGTGCAGACGCACTCGCACGTCTACGAGTCAGATCCCTCGGACTACTCCCAGTTCGTGGGCAGCGGTAGCTACTCCAACTCGTACTCCCCGTACAGCTCCGGATCGGGGGCTCCTCACGAGTTGGGCGGCAATAGCTACAGCAAGGACTGGGCCACGAGTAAGGCCTACAACGCGCACAACTATCTTGACACGATCAGCAAGCGGATACAGTAGAGGATCCGAGGAACCAAACGGACTTGTGCCAACTATACCGAAACCTAGTAAATTGTTACTCGTCGGCGC
 
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