CR60272
CRIMIC CR60272
CG8866
Information
| Donor |
Location |
Cytology |
FBti |
Stock number |
| pM37_p2
| 3R:.. | | | 94368
|
PCR Verification
PCR check relative to sgRNA sequence
| HA-L |
HA-R |
Forward |
Reverse |
| no band |
OK |
CCGTTAGTAAGCGAGTTCTGTGGA |
TAGGACAAGGCACGATTGCGCAAT |
Images
Construct Design Sequences
| sgRNA sequence |
ATATGTAGGAGTTGACATGGTGG
|
| sgRNA2 sequence |
|
| Amplicon PCR 5' Left |
Amplicon PCR 5' Right |
Amplicon PCR 3' Left |
Amplicon PCR 3' Right |
|
|
|
|
| Amplicon 5' |
Amplicon 3' |
| GCTTAACGTTTTCGAAGACTTTAGCCGCGTTTTAAACACTTTCCAAGGTGGTTAACTACATATATTTTTATGTTATACTGTTGCAGTATCTGCAGAGGCTCCGAAAAACAATAATTGAAATACCATAGCATTTATTTACCCCAAAAAAACGAAATATTAATCACCACGCAACGCATCAAAAACAAAACATACGAATCAGGGCTTAGATATTTCCAGGGATGCGATAAGCAAAAGTAGCAAATACCGATAGAGCAACAAGTGCTATCGATGTTGTCAGCATTGGCTAATCAGCTGTGCAGTTGGGCGCCTGTTTATTTATCAGTTTAAATGTAATATAGGATATAAGAAAAATGATTAAATTGGTATTCATAACTGAGATGCATATGGTTACGAGACGTTCAGTTCATAAACTTATAAAATTGAAAAAAATAATATAGATTTGGTTCATTGTAAATTTGAAGTTATTGCCATGTTTATAAAACAACATAACCGAACTTCATTTATGTCTGCTGGATTAGTTAAATCATATATACATTTTAATTCTGATTCCTGAGTAAAAACTTTTTATATATTCAACAAAATCAAATTTTTTATATCGCCGAATGATTTAAATTCGATTGATTGCTATCGATTTGTTTCAGCAATTTCACTGTGATATCGTTAAGAATTGCGAAGCCCTGGATTGCGCGCGCGTGTGTGTGTGAGCAGAGGAGAGCGAAAACAATCGACCAATGGAAAACATAAACATATAATTTTAAAAATATTTGGTATCAGAACTAGATGACCGGCCCAACATGTCACTGCCGCGGATCACGGACTTTGAAATTCTCGAGAAACTTGGAGCGGGCAGCTATGCGACCGTGTACAAAGCGCGGCACAAAGTGGGACACCAGTTACATACACATCACATAGAGCAAAGGGTCTTAATATTTACGTTTTGTCATTGCAGAAACAGCGCACTTATCATGCCATCAAGTATGTGGAAATGTCGACGTTGTCGCAAACCTCGCGGGAAAACCTAATCACCGAAATACGCCTCCTCCGGGAACTCAAGCACAAGTACATTGTGACCCTGCAGGATTTCTTCTGGGACGACAAGTATGACATCGTTCCATGCAATCAGTCGATGTTCATCCTTCTAGAAGG
|
GGTGGCAGGATTAGCTAACAATCCTCTATAGCTCTACTCCTACTATGTATATGTACATAAAAAACGTTTGGTTCTTTAGAGTTCTTCAATTCAAATGATAGTTTTGTACACTTATTCTACTCAAGACATACTCAGTTACAAGGAACGGGTTCCAACAATTAATCATCCAGTTTTGACCAATTCAGCGGAACTAGTTAATTGAAATCGATTTTCGTTAGAAAACATACTAGCAATGAACAGAATTAAGAATGCTACAATGTCTACTTTATTTCAGAAATATTTACATCGTTTTGGAGTATTGCAATGCCGGCAATCTATCCGCTTTTATACGAACCAAGAAGGCATTGCCGGAGAGCACCTGTCGCTACTTTTTGCGCCAATTAGCTGCCGCTGTGCAGTACATGCGAGCCAACGATGTGTCCCACTTCGACCTTAAGCCACAGAACCTGCTGCTTACCCGGGGAGCTAACAATGTGTCCCTGAAGGTGGCAGACTTTGGGTAGTTAGCCAAATAGCATTACAATAGATACTTTATACTAATAATTCTAGTTTGTTGTAGGTTTGCTCAGCACCTCAAACTAGGCGAAATCAATCAACAGCTGAAGGGATCGCCGCTCTACATGGCCCCAGAGATTGTGCGCAAGCATCAGTACGATGCCAAGGCGGATCTCTGGAGCATCGGGGTCATCCTCTACGAATGCCTATTCGGCAAGGCGCCGTATAGTTCGCGAACCATTGAGGAGCTATTGCTGAGGATTAGGAAGGCCGAGGCCATAACACTGCCACCAAACGCTCGGATCAGCAACGAGTGCCATGATCTCTTGCGCCGCCTTTTGGCCCACGAGCCCACGGCGCGCATTTCGTTTGCAGACTTCTTTGCGCATCCCTTTCTCGATCTCAAGACGTTTCCCACGGAGCACACTCTGCAAAAGGCCATTGATTTGGTCACGCAAGCATGCGCATACGATGAGAAACACAATTACAAGGAGGCTTACTATCTGTACTGCAGTGC
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