CR60275

CRIMIC CR60275

Gene Hr83

CG10296

Information

Donor Location Cytology FBti Stock number
pM37_p1 3R:..FBti0219213 Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK OK CGGTCCGCAATAGTAGTAGATG AAAGCGCAGCAATCTTATGGCG

Images

Construct Design Sequences

sgRNA sequence AATCTATACATAGCGTACATAGG
sgRNA2 sequence
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
 
Amplicon 5' Amplicon 3'
GGCCCTACTACACTGGGAGAAATCTATATCAAATTTTTTTGGATTTATATCTTAACTTGACTACGACTAAATATTTAAATACGAGATTGATATATAACAAAAACTTATCGTATACCGATACTGTTTAATTTTTATCAAACTATATATATTTCTTACACAAATATCTTAATTATTTTAAAAGGTTTCTGGTGCTTGCATTCTAGTTTTGAAAGTCCTAAGTTTGAATAAAATATATTTTAGTGATATTTCGCTTTTAGGAATTTGTTCTTTGGATCCCATTGAAATACAATTTCCATCTCTAGTTAGGAAACATATTTTTGCTCTGTGTAACTGGTTTGCATATCATTTGCGGGTGTAGCACATCCAGGTGGCATATAAAACCGCCAATATCCGGGATGAGCTGATTCAGTCCGCGGCGACTCTTTGGCTCATTGGCAAGCAGTGCCAACCGGTGACCATGTCGAACTTCAGTGCCTGCGCAGTGTGCGGCGATCAGAGCTCCGGGAAGCACTACGGCGTGTCCTGCTGCGATGGGTGCTCCTGCTTTTTCAAGCGGAGCGTGCGGCGCGGGAGCAGCTACGCCTGCATCGCTCTGGTCGGGAACTGTGTGGTGGACAAGGCGCGGCGGAACTGGTGTCCCTCCTGCCGCTTCCAGCGATGCCTGGCCGTGGGAATGAACGCTGCTGCGGTTCAGGAGGAGCGCGGTCCGCGCAACCAGCAGGTGGCTCTCTACCGCACTGGCCGGAGACAAGCTCCGCCATCTCAGGCGGCGCCATCCCCGACGCCCCACTCCCAGGCGCTGCACTTCCAGATCCTCGCCCAGATCCTTGTCACGTGCCTGCGCCAGGCGAAGGCCAACGAGCAGTTCGCTCTGTTGGATCGCTGCCAACAAGACGCCATCTTTCAGGTGGTGTGGAGCGAGATCTTCGTCCTGCGAGCGTCCCACTGGTCTCTGGACATCAGCGCCATGATCGACGGCTGCGGCGATGAGCAGCTCAAACGGCTCATTTGCGAGGCCCACCAGCTAAGGGCCGACGTCCTGGAACTCAACTTTATGGAGTCCCTAATCCTGTGCAGAAAAGGTAAAGCTCCGCCTGTGCGGTAAAAAGGCTTGTTAGGATTTAAAAATAAAAGCGGTTTGAAGCCATCTAACC CTGCATGAAAGAGTACACCCAAGCAGTTTTCTTAACCCCAGAAAAAACGTTTCCGTTTTGGGCTTAATAAATTCAAAAGAAAATTTTCCAGCATGTGCAGTTGGTTTGTTTGAACCAACCATAAAATTTGATTAGATTCAAGATTAGAGACAGATTTAAGACCTTTTACATCGTTTTAATTTAATTAAGAGTTTAATAGGAAGTCAAACCACCATTAGTATATCCCATTTTAATACTTTTAATTGCTTATATCGATTGCATGTTTTTACAGAATTGGCCATCAATGCGGAGTATGCCGTTATCCTGGGAAGCCACTCTAAAGCCGCCCTGATCTCCTTAGCCCGCTACACCCTGCAGCAATCCAACTACCTGCGGTTCGGACAACTGCTCCTTGGTCTGAGGCAGCTGTGCCTGAGGCGCTTCGACTGCGCGCTTTCTTGTATGTTTCGCAGCGTGGTCAGGGACATCTTAAAAACACTTTAGGCTCATATCTATCTTCATCTTTCAAAATTAAAGAAAAACCTAGTCTTAAAGAGGGTACTAGATAATGGCTGTTTTTCTGTTCGCCAGTTCAAACATCTAAAGTAACTTCGCTATCTGAAGTTTCAACCCATATCTAAAAATGCATTCATTTCTGTCAACAATCGTTCATGGAGGCATTTAGACACGCCCCAAACTTTATTGGCACTATGCAATTTGTTGGAAATAAAAATATTTGGAACAAAGAGATTATTATGAACTCAAAATTTGATGTGGTATTTTTTGAAAAACACTTTTTCAAAGGTCGCCAATCACAATACAAAAAACTCATCGATATGTTGTGAAAAGTCACGGAGCGAATATATTCGTTTCTGTTCGCTATCGATATATTGATATATTTTTGCCATCGCTACATTGTCTGCTGCGGCCGTCTTGTCGTCTTTCTGTAATTATTTTTCGTAGTTAACAAACCCCTGAATAATGTAAACCAATTAGTGGGTGATGCAAGGACCGAAAATGCGCGCGCGTTGCTAAAGAAGC
 
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