CR60280

CRIMIC CR60280

Gene CG2616

CG2616

Information

Donor Location Cytology FBti Stock number
pM37_p2 3R:..FBti0219215 Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK OK TAATTGCTTTGGCAGGAGCGAG ACCTGCCTCATGGCTCTTTCTTTG

Images

Construct Design Sequences

sgRNA sequence AAGCTGCAGAGTTTAGCAAATGG
sgRNA2 sequence
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
 
Amplicon 5' Amplicon 3'
CGGGAAGAGCTAACGATGGAATGGCAAATCATATAAATAAAGAGGAATTTTCTGAATGGAGTGTATAATCGCTAAAAACGAAGCAAAGTGAATGAGCAACTTTTTTTCGCCGTGAATAATTGAATGAAAACATTAACAATTGCCAACGTTTTGATTGTCCATCAAGTGTAAACGAAGTCAATTTTTTTAGACCAAGTAAAGTCTGTTCGGGGTTTACTTAAAAAAAAAAAAATAAAGTGGGGAACTCTTTATTGACTTTATTTATTTTCATCAATTTATTGTTTTATTTTTTTTTTCAACTAAAATTTAATCATAAGAATTAAGTTATCCTTTCTCTTTGGCAAGTGTATACCAACTCTATTTCTTCCCCTGTAGAGTTGCCATCGTAAACGTCATGTTTATCGGAAAATTCTTTTGGCACCAAGCAATGTGAAATTTATAAATGTAAAGTTACAAATGCTACTGGAAACCAGATCGACTCTCCCTCAACTGTTCTAGCGCAACGGAGACGGTCGCCATGTCGAAGGAAGACTCAGGCGCATCCAGTGCAGAGGCGAATCCAGCTGAATCGGAAATTTCGATGGGTCGAGGACCCCGCCCAAGGTCCTTTACTGGCGGCGGAGGCGGTGGCGGAACAGGTGGATCAGGTGGAAGTGGTGGTACCTCCGGCGGCAACAATCTCAAGCCCGAAAGAAGCGCCCGGGAAGATGACGCCATCAACCGTCTGACGGACTCGAAGTCGAGTCACCGAAAGCTGCTCAGCGATCCGCGATTCCAGATTCGGCCGCTGCAGCAGGTAATCTCGGCCTGCACCGGGGCCATGATCACGGCCTGCTTCATGACGCCCCTGGACGTGATCAAGACTCGCATGCAGTCGCAGCAGTCGCCGGCCCACAAGTGCTTCTTCTATTCGAACGGGCTGATGGACCACCTCTTCGCAAGTGGACCCAATGGCTCCGAGTTGGCGAGCCTGCGGCAAAGACCACAGTTTTCCAGCTCCTGGGATGCTCTGATGAAAATCAGCCGGCACGAAGGATTGGCCGCTCTTTGGTCCGGCCTGGGTCCCACCCTCGTCTCCGCCCTGCCCTCCACCATAATCTATTTCGTGGCCTATGAACAGTTCAAGGCCAGATACCTCCAGATTTACGAGAGCCACTATAATAAGAGTCAGGAGCCCCGGCACCTTGAAATCAGGTACTTACGTAACCCTTTAAATACATAGTGTAATACATACATGTAGTTTACCTGTTGAAGCGCTCATTAAGC GGAAGAGAATAATCCGCAGCTAGCTGTTATTGTGTTCACAGAGAACTACCTGGGGAATACTAGTTTAAAAATATTTATTTTTCGTTTCATTTCGCTATTTCAAATGAAAAACATCTAAATTAAGGTTTATCAATACACATGCACATACAATACACATATTTCCACTGTATTAGACTGGATAGAAAATGTTTTGATGTATTGTACGATTTATTCGCAACTTCACAACCTATATATAAACCTATATAAGAATATGATTTAAGGATTTACAGGTTTAAACGTTTTCCATACTTCTAGGGATACCAAGAAAAGTCTACCGTCGGTGGTTCCCATGATGTCCGGGGTGACTGCTCGGATTTGTGCTGTTACCGTGGTGAGTCCCATCGAGCTGGTGAGAACCAAGATGCAGGCCCAGCGCCAGACCTACGCCCAGATGCTGCAGTTCGTCCGGAGTGTGGTGGCCCTTCAGGGCGTGTGGGGCTTGTGGCGGGGCCTGCGTCCCACGATCCTCAGGGACGTGCCCTTTTCAGGGATTTACTGGCCCATCTATGAGAGCCTAAAACAGAATCTCGGCCATGGCTCACAGCCCTCGTTCAGTCTGAGTTTCTTGGCAGGCGTGATGGCAGGGACAGTGGCCGCCATTGTGACCACTCCTTTCGACGTGGTCAAGACACATGAGCAGATTGAGTTTGGAGAAAGGGTCATCTTCACGGACTCGCCGGCGAGGGATTTTGGAAAGAAGTCGACCTTCAGCAGACTAACGGGTATTTACAGGACACACGGCGTGCGGGGACTCTTCGCAGGATGCGGACCCAGGCTTCTGAAGGTGGCACCTGCCTGCGCCATTATGATCTCCACCTTCGAGTACAGCAAGTCCTTCTTCTTCCATTACAACGTGAGGCATCATAACGAGGCCCTACTCCTGGACAACCCGAAAGACACGACGGTCGAAGATGATGACATTGAGTAGAGAGATGGGTGGCTCCTTTGTAAATAAAGAGCGCATATTTTATGTAATCCCCGGGTGGCGAGGCGAATTCAATATCCCCAACGCTTTACGAGTTACGTGTTACGTCAATAAGGCGAAGCCGGGAAATCAATTTCCATTTTCGCAGCTTAAAGTCTCCGGCTGCCTGTTGCTGGTGCTCCGGCGATACCGGGAATGCCACTC
 
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