CR60284

CRIMIC CR60284

Gene CG13995

CG13995

Information

Donor Location Cytology FBti Stock number
pM37_p2 2L :..Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK OK AACAGGGACAATCTGCAGCAGT GCTTTCGTTACCAGTGCTATCC

Images

Construct Design Sequences

sgRNA sequence TATTCTGACCAAAGTCAACGGGG
sgRNA2 sequence
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
 
Amplicon 5' Amplicon 3'
GGAGAACTCGTATCGGCGCCAACATCCAGAGCCCACCGATGACCTCGGCCTGGACTCCGCGGAGCTGCATCTCGCCCTCCAGGAGCCCAACCAGCTGCCCGCGGACTACGACTATGGCAACTTTAGTCTAGGCAATCCGTATGACGTGGACTCAGAGCACTCCATTTCCCCGCTGACGCTGCTCCTGCTCGCTGTTAGCTATGGCCTGGTGGTTTTCGGCGGCGTCGTGGGCAACTCCACGCTCGTTCTGACCCTCTGCTCCGCCTCTTCGGTGCGTTTGCGTAACCCGCTGCTGCTGGCCGTCTGCATTGCCGATCTCCTGGTCACCGGCATCTCCGCTCCGGTCACGCTCCTCAATCTCGCGATGAACCGGAGGACGCGATCGCTGCCCCTTGTGCTGTGCAAGGTCATACACTACGTACAGGTGAGTGGGTCAATCAAACAAGCTTAAGTTAGCCAAGTAACTAAGTTAACCCCACGCTATTCAGGTCATGCCCGTTTCGGCCAGCACTATTTCGTTTTTCATGCTCTCCCTTGACCGCTACGCCACAGTAAAGCATCCGCGATTGGCGCAGCTTCGCCAGCGGCGCTACTTGCACGTCTCCCTGGCGCTGCTATCCTGGCTCGCCTCTGCCGCCATCAGCACTCCCTTTCTGTTTGCCTACAAAATCATCGCCAAGTCGATGGTGGTCAAGGGCGGTGGGGCGGCGAACACCACGCCAAACCCGGTCAGCATCAGCTGCACCTCCGACCTGGGCGCCAACGCCATGTTCATGTCCTTCATCATCTTCCACACCATCGCCGTGTTCGTGTTGCCTGGCATTGGTGTGCTACTCAACCACTACGGCGTCCGACGCAAGCTCTGCGCCCTGTCGCTAACGGCTCGAGCTGCCCATGGAGAGCTGCCGCTGCCCATTCCCATCCTGCGACGCCAGACCCACATGGTCATTGTGACGGGCTGCCCCAACGCCCAGCAAGCGGCCTGTGGTGGGGGCACCACGGCGGACGACACCTCGAACGGGAACGGCACGGGCACCGGTGGCGGTCCCATGGCCGTCAGTCCGGGCGACATACAGCTTCACACACTGCAGCCGCGCCAGCCAGGCTCTGCCGGATCGGCCCTCGAACCGGGCTCCTACCGCTCTAGCAATCCCATATCACCCAGG CCACTTAGGGCCATGAGGGAGATTCGCGCCCACTCGCAGCGCCAGCGCATCAACCGAGCAGGCAGGGGACCAGCGACACCCGGCATCCCACTGCCCCAAACCTCCACGCTGCGGTCGCGAAGGCACCTGGCTAACATGCTCATCGCCTCGGCCGTGATCTTCATTGCCTGCTGGGCACCGCACGTCTTCTGCATCTTCTACAAGAACTTCGGCAACAATCAGCAGTGCAGCCAGACCTCTGTTTACTTCAGCCTTCTGCTAGGTAAGTCTGAATTGGCAATCATTCCCAATCCGAATCCGAGTCCGAATTGGAACTGACTAGTTGATCGCGTTGCAGGCTACTTCTACTCGGCCATCAGTCCGGTGATCTACTGGGCGCTCAACCACAATTCGCTTCGACAATCTCCCTGCGCGCCCATCATCCGCCTGCGCTCCATGCAGAACTTCCTGAGGTCGCGCTTCCGGACGCACACCGCCCCGCCGCCTCCCTCGTCCACCAACGAGGCGGCACTGGGGGCATTCAACCCCAAGCTGATCAAGCTAACACCGAAACAGTATAGAGCTCAGGCCTCTTCGCATTATCTCTACTAGTGTGATACTAAGCTAAACTTGATATACATATATACACATTTATATACACGCATATATAGTATATTTTTTACCTACGACCCCACCCCCCCCCTGACCCGTCGATGCCCTCTTAGTACCCTCTAGTATCGAACTCCGAACCCGAACTTTGACCCAACCGAACGAAACTATCTAGATGTTTTTCAAAAGCTCTACGCAACAATTGCATAATACGAAAGATACATGCTACATATACACATATATAGAGTACATACTACCTTGCATAGCAGTGTTAGTCGACGCTTATTTACACGATGTTGTGCGCCTGAAGCTATGCAATAAGGACACCGGAGTCCTGCCG
 
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