CR60285

CRIMIC CR60285

Gene CG14339

CG14339

Information

Donor Location Cytology FBti Stock number
pM37_p0 2L :..Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK OK CTGTGGAGGTGTTCCAAACGTT CAGGTCCAAGAGGTCCAGTTTGTA

Images

Construct Design Sequences

sgRNA sequence TAAAGAATTAATCATTAAGGTGG
sgRNA2 sequence
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
 
Amplicon 5' Amplicon 3'
CATGAGATTCGCTGTCCAGCTGGACTACCTCGGAAAACTTAAGCCCAAGAAAATAATGAGCACGTTCAATTTGAAGTCTATGGAGCGTGCGAAAACCCTTCCCACATTTGCCATCATCAAACTGTGCCTCCTGGCCCCGATTGGAGAGATCTACAAGGAGCTGAAGGTGTTCCGGGAAAGCTTCATCCGGCAGAACCGGCTGCTCTTCTGCGACCGTCCTTACAGTAGCATCAATGCGATGAGCCTGGCCGAAATCCAAATGGAGTGCTACGCTCGCTTCGACAAGTTCATCCGCGACTGCATATCCTTCATCATCGACAAGAAGGTGATGAAGCTGGAGGACAAGAAGCAGCACTTTTGCTGTGCTGTGCAAAACTTGACCAACATTCTGATGAAGGTGGTGGGTAGTGTCTACAATACCAGGACGCCCACGAATACAAGCGTAGACTTTGCGGTGCGTAGTCCTCTGGAATATTGAATATTATGTTTCGTTGCTTCTCCAATCTCCAGAATCTCTGTGCATTTGCCTACAACGAGTTGGAGCCAAGGGTCCACACAGTGGTGGAGCAGATCGAGAACGAGGGTTTCGATAGCTACACGATCAGTAAGCAGATCCAGAATGAGCGAATCATCGACTACTTGAACACCATCAAGTTGCTCCTCATGGTGAATGATGAAAACCATGCTCACCTGTTGATGGAGAAGGTCAGTTGATTGATTCATTCGTTGGAAATGTGAATCATTGATGGGTTTTGGGCGCTTTTATTGACCAACCCCACCATTAAAAACTTTCTTTATAAATTCGTAAGCTTTCCAATGAAGTTCTAACTAGATAATTATATTCTATCTATAAAACAAATGACCATCAACAGTCATTCAGCAATATTTATACTCACTAATTTAGTGGCTTAAAAATTATTGAATTAAAATTGATCTGAGTCATGTAAGATTAAATTAACAAACAGATTTCAAATTCCGATTAACTTTCGATATGATATAACCGGAAAGGAAAAAGCTTCATCAAAACCTGTAAGTGATTCACATGAATTGTACGC GGTGGTTAGGTGCTGAGTTTGTCAACAATCAAATGAAATTTAAAAGTAATTCAATGCAATGAAAAAAATTCAATGAAAGTAATGAAAAACCCTGATGATGGAACCATTTAGCACTTCCGTTGCCAATTTCCTTTAACTATTTTCCATTTCCAGGCCATCACGGAGTATCCAAGTAACGAGCGCTTCTGGTTCTACATGGTAATCGCGTACATGGAACGCGGCGAGCTGGAGAAGGCGAAAGTGTACTTTAAAAAGAATCACCTGGCGGACACCCACGACTATTTCGCGTAAGCCTCTTGATATTCCACAGAATGAAAATGAAAATAGCGATATGATATCACAGGGGCTGGATTAAGCTGTACATCAACTACGTGGACACGCGAAACAACCCGGAGACAGCGCCCGACTGCACGGAGTGCCTGTTGAGGAGCATCACCAACTACGCCGAGCGGTATCCCCGCCAGCAGGACGCGTGGATCCTGCTCTACTGCTACTACAAGCGATTCAACTACGAGCCGGGCTGCGCCTTTGCGCTCTGGAGATTCGGGGATCAGCATGGGCACTGCCGACTCAGCTCCTCCTCCAATGCACCGCACAGCCTGTGGGGACTCTATGTGAACCTAAACATCACCATTCCAACCAGTCGGGGCACCATGTTTCTGGAGGTCTTTAGAACATTCGCGCGGCTGGGCCTCTATGAGTTCGCCCAGGTGGTCATCGCCGCCGTGTTGCCTCTGGCCAGCGATGCGGATAGGTATATGCTCCAGACCCAGCTGGACATGATGCTCAACCAACTGGACGAGAACTTTGTGCCCCTGAGCTTTGAGTTTGAGGAGGGCGAGGAAGGTGACTATTCGGTGGGTTGTTCATCCTTCTTTTGAGGATCTTTGAATTGAGTCCGATTGACTCCCTTGCAGGCCGCCATGAATGCCCAGATCAACGGCAATGTGGAGTTCCAGCGAGGGAACCTGGAGGAGGCGGCCTTGTACTACGAGAGCGCCCTGACCTTGCCGCCTCCCGAGGTGAACGAGCGCGACTTCTTCGAGGTCAGCCGACTGCGCCTGGGCTACATATCCTACGAGCTGGGCAACTACAACAAG
 
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