CR60287

CRIMIC CR60287

Gene CG3515

CG3515

Information

Donor Location Cytology FBti Stock number
pM37_p0 2L :..Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK OK TCAGCTTTCACAGCTGCAACTGCT ACTGGCCAGGAAGTTTGGGTGATA

Images

Construct Design Sequences

sgRNA sequence AAGAGACACGGCTTCGAAAGAGG
sgRNA2 sequence
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
 
Amplicon 5' Amplicon 3'
GAGGAGAGCGGGTCCTCCGTTGCACTAATGACACAAATCGGCCTAAATAACAAGTTGCTGGCATTTGGTCAACAAGTGGAGATTGGAGCGAATGGGGCGAGCTGCGTTGCCAACTCAGCAGACAGTTTTTCCTTCAATTTCCAGCCAAAACCACACACCACCCACGGCCCTAATGAGGCGCAATGATGGGGAACATGGCTATCTGGGAGCTGGTCGCGAAAAGGAAATTAAAATGCATCCCCAGCTGTGCCAGTGGGCGATCCGGTGGGCGTGGCAGACGGTTCAACACTTGATTTAATCATTTTGATCCGGGGCAAACTCAAATCGGCTTAATTTAGTTTTAAAGTCAGTCAGTTGTAATAAAGTTAGTGGCATTTGGCCGCGAGGAAGAGGAAATCAGTTGAATTTGCTAAGAAAGGATCAATTTTCTGTCGCTTTCGTATAATAAAAATTTAAAACAATATACTCTCGAGAATTACTTCAAAATGACAGATTGAAATAATTTTAATGACAGGCCTTAACATCACATTCTTATTGAAGCTCATTTTCTCACAGTACATATTTAAAGGAAATGTCTATCAGACTGAACGATGACGCGATGCGGCAGTTCCGGGTGGCCAAGGTGTTCCCGGAGACAAAGCACCACACGCTTGCAATGGAGTTCTCCTCGGACGGACTGCGCCTGCTGGTCTGCGACCACTCCGCCCTCTCGATCTTCAGCAGCACTCGACAGGTGCAGGTCTGCAAGGTGCCTATGCACCACTACCTGCCCGACGTGGCCTGCTTCGTCCAGCAGGACACTCGCGTCCTGCACTCCACCTCAAAGGTAAGAAACTTTAAAGGGGAACGGTGGGGCCAAGATACACAGAGTGAATGGTTAGAAGAGACACGGC CCATCGACAGCATGACTACACCATCCGATGCCTGGACCTTAATACCCGCCACTGCGTTCGAGTTTTCGGTGGCCATGCAAAGACTATCAATAGGCTGGCCTCCCAGCCTGGAGGCGAGAATGTGTTCATCAGTGCCGGGCGGGATGATCAGGTTTACATGTGGGACATTCGGGTCAAAACTCACACACACCGCATAAAGAATCTGCGACGGCCACTATGCGCTTTTGATCCTGAGGGCTTGTTCCTGGCCACCAGTTCGGGCACGGAGGGCATCCAGATCCGCGATGTGAGGATGATGGGCGGTGAGCCGAGCCGAAAGTTCGTATACCAGGTGAACGCCAAGGCCAATTGGACACAGTTGCAGTTCGCTCCCAACGGGAAGAGCCTGCTGCTGAGCACGGATCACTCCTGGTGCTTCAGTGTCTCGGCCATTGATGGTACCTACCAACAGTCCTTTACTGGCTATTCGAATAAGTTAAGGCTGCCGTTGGATGCCACTTACACGCCGGACTCGCAGTTCATCCTTTCGGGAGCGGATAAGGGCAGGATTCACATTTGGCGCGCTGCCGATGGATTTCCTGTGGCAGTGCTTAAGGGCAACAATATGGGTCCTGTTCGATGCATGCGCTTCAATCCGAGAGCCACCATGTTCGTGAGCAGCGACTCACGAATCGTGTTCTGGATGCCCATGGCGAATGGAGTTTACGATTGGGTGAAACGCCTGAAGCCCGGCGAAACTATCTCCTTTACGGACGAAACTGTACAGGAGGAGGCTGTCAAGGCTCCTAGGACGATTCCTATGCCCAAGCCCAAAATTGAAACGGTATGGAAAATAAAGAGACTGAACAGAAAGAGAGCTTGCACAGGAGATGAAATAGTTGATCTCACCAATGCCCTACACAATAATGACTCCATGGAAGATGGCGAAATAGCCGAAGACTAAAATGTGTACCGATCTTTTGGTAGAATAAAGGCACGGGTTGTTCATCAGCTGGCTGCTGGCCAAAAGTTTGCAGACCAACTCGCAGCTGG
 
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