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CRIMIC CR60292

Gene CG6431

CG6431

Information

Donor	Location	Cytology	FBti	Stock number
pM37_p1	2L :..			Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK

Images

Construct Design Sequences

sgRNA sequence	TCTACGGTTTTATACACACATGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right

 

Amplicon 5'

Amplicon 3'

CCAGTCCACATATCGATCACCGTCTGTATGCACCGTAAGTTCGATTGGATTCGAAGATATTATGAGTACTCCCAAAGTAATATTCCTTTTTGAGCAAAATCAAGTTTTATCGGTGAATCACACTTTTAAATTGGTATTAAGTCCATAAAGTGCTGATCAAAATGCTTGCAAATATATATTTTTTAAGTGCTAAAGATGTGTTTTCATCTTTAAAAATCAGTCGTGCCACATGTTTAGGTTCGATTTGTTTATAAATTTAAATGCCAAAAGCAGGAAAATTAATGTTATTAATTTAGTCGCTGGCGGGGGAGAAAGTTCCTCAGGTGTTAGGCAACTGGAATTTCAGACTCGGTTAGTGTGTCAACTGCCGAAACACTTGTCGCACTGCACTTGTGCAACCATAAGGATATTAAGCGGCAAAGAGCAAGAGAAAAAATAAGAGTACAATTAATGGGCGGGCAACTCGTTTCTCGAAACCAGCCACTGACCCACTAAGGATGGGATGCTTTTGCCAAAGGAGGCCGGCTTTTTCCACATCGAGCGCCGATCTTGGCCAGCGATGACATCAATTTCAATGGCCAATGCCAATAAAATGCTTTGTAAATGGCTCCTCGATATTTGCCCAACTTGTCTGGGCAAGATTACCGCCAGCTCTGGAGGCGAGACAAAGTCGCAACTGGCGAAGTCCATTTTTGGAGAGCAGACGGGTAATTGCGGTGGTAACAAATTTAACAACTGGGGGGATTACATGATGGCCAGCTTTTACGAGCCTGCTGATTGATGGTTGTATAACAGCGGGAAATGACCGGGCTGACACCTCCGTTCTGGGAAATCCGCGAAGGAATACAAATTGCTTTTTCTGCAACTATTTAGTCTCGTTAAGAATTTCTGGAAACCGTTTGGCTTGTAATTGGTGATGTATTTTAACTCATAGTTGTAAAGTCTATAATTTGATCCACTTTAAATGAATTTTTCATTATTACTAGCTTGATATTCAATTGAAAATCTTTCAAGATTAACTAAAGTTAAGAGTTAGCATGCCCAGATCTGTGCTTG

CAGCAATGGACCGCGTCGTGGAACTCCTTTAAATGTTAAGAACCCAATAACGCTTTATAAAGGTGGATTTAGCAAGCACCGCGAGACTGTCTTCATAATACACGGATTCAATGGAACTGCCATAGATATTCACCTGCAATTCCTTCGAGATGGTGAGTGCATCTATCTGTTAAATTATTATATTCTACAATATTAAGCTATAATTTATATAACTTTAACTCTCTCTTTTTAGCTTACCTATCCCGCGATTTCAATGTCATTACAGTGGACTGGCGACCTTTGACCCGCTATCCATGCTACCTCCACTCGCTTATAAACACCAGGTTAACTGCGCAATGCACCGCCCAGATCTACGCCTTCCTCACCCACTACGGAGCTGTCCGGGAACGGATCACTTGCGTGGGTCACTCCCTGGGCGCCCACATCTGCGGCATGATCAGCAACCACCTGACGCGGAAACAGTATCGCATCATTGGATTGGATCCCGCCCGGCCTTTAATTGAGCGCATGAAGAGCAACAAGTTTCGGTTGTCGATCGATGATGCCAACGTCATTCAGGTGCTCCACACCAATGCCGGTTTCCTGGGCCAGGAGGATAACTCCGGGCATCTAAACTACTGTGTCAACGGTGGACGCATCCAGCCGTTCTGCAAAGGAAATCCAATCAGTAAGGCTAAATTAAAGTAGTTTAATACCCTCTTTCTCGGAAAAAGAAATCATATATAAATAAATGTGCTCTCTATCAAACAGGAAAATCGCGATGTTCGCACTTCTTGAGCATCTGCTACTTGGCCACTGCCACATTTAAACACAATAAGTTCATGGGAGTACCGTGTCCCAATGGCTGCCTGAATCTTAGTGGATCCAAGAGGCTGCCAGTGAGCGGCAAAGTGAATCCCTTCGAGTTCGCCTCGCTGATAAGGGAGTACCACATAGGCAACGATGCCCCCGATGAGTAAGTTTATTAAAAACCATATATATAATTTATAACCAATTTCATATTTGATTTAGCGCCCGAGGCTGCATTTGCATTGATGTGCCGTATGCCAAGCACTGCCCCTTTAC

 

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