CR60294

CRIMIC CR60294

Gene GATAd

CG5034

Information

Donor Location Cytology FBti Stock number
pM37_p0 2L :.. FBti0219219 Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK OK CACAGAAGCACATTAGCATAATCACTG AGAGAACGTGCTTAACTCCGTCAG

Images

CR60294.jpg

Construct Design Sequences

sgRNA sequence GATATATCCAACTCACACACAGG
sgRNA2 sequence
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
 
Amplicon 5' Amplicon 3'
GCCGAGATGAATCATGCAGCTAACGCGACTATCGCTGGAGCCCTCGTTTGGTAATTTGAACGGGAAACTAGATCAAATGAGCGAAAAATCTGTGGCGAACCACTGGAGTGAATTATTTTTAGTGAGCTGAACGTGGATTGTGACAATGAAAAATTGAGGAATAGTGTTTACATTCATTTTATAATTTATTTTACCAGTTATCTTTTTTATCGCGATAAAGGAGAGGCGTATGAAAGTTTCTTTACACAGTGCAAAACGAATATAAATTTCTAATTATGAATAATGTACCACATAAGTTTCGTCAAGTTTGTCGATTGTGCCTCACATTGGTTAACGAGTGCGATGTTGCAGTACTACAGATTTACGATAATTCGTCGCATAATAATAGTGCCCCAGACAGAATATCGGCCGATTTTCGTGGCGTCGCGAATCGTGCCAACTGCTTTTGCAGCGCTTTAAGCCCCAATCCGTGCAGTTGCATCGTTGATAACCCGCTATCAGCCAACAAAAATGAACGGCGCAACCAGGGAACACCCGTACCCGTGCCCATACCCATACCCGTTTCCGTGCCTGTGCCCGTTCCAAGCCAGCAGATCCAATCGCAAACCCTTCACCATCACCAAAACCAAACTAAAATCCATTACACGCCAAGTGTGCCTTCTGCTGCGGACGCAAAGTCGAGTGAGCGCGAACGCCAATGCCAATTGGAGTCGGACATCGCCGAGAGATCAACAGTATTCAAAAGTCAGTCATCGTCGGATCACGAACATATAGAATATAGCGGGGAGACGCCATCCCCCTCCGCATCGCTATCCCAATCCCAAACAAAATCGGAGCCAATGCGCGAGGAGGAGGAACATCAGCATAATCATAATCAGCAGAGCGGACGCGAAGAGACAAGTGTGAATACAACAAATATAAGCGGAACCGATAACTACGGCAAAGAGCACGGTCGGGATGATTCATCATCACCACATCTTACATTTCAGATATTCAACTGTCTCTCTATTAAGGTAAGCAGATTTCCATCCAACACATTTGCAAAGAAACGAATTTACATTAATTACGCACATACACAAGCATGCTCTTACC GATAATGCCCGCACACACGCGCACTTTTGCGAGTGCTTGCCGTGCGCTGGTGTGTGTTATCTATGTGATTACGACACCACACGTCAAAATAATAGGAAATGATTGGGCCGCTTTTATCCCATGGCCTATTAACAATCGGTATCAATTGTTTGGTATTTTTTCGATGAACCATTGCAAGGCATTGGCCCAGGCCCACGCACACCACGCCACACTCGTACATCAGTGTCCAGCGCACGAATGTAAATATTGCTATGTACATACGTGTGTGTATATGCACGCATGGATGTATGTTAGAGACCTACATACATTAATCCACCAAAGAGATAACATTCGTTTCGATTGTTTAAGTGCGGATATACCGACGTACATACACCTTATATGGCCCCAATGTGTTTTTTTTACATACTTCGAGTACTTTCATTTGTTATTATGTATTTATTTATTTATTGATTTGATAATTTGTGCTCTTAATGCATTTCATGAGCTTTAGTGTTTATTTCCGTAGATGAACATATGTTATTTTTTTTTTTTTTTATCATTTTATAGGGTTGTTTTTAATTTTTTTCAATTAAAATAATTTGTTTCATGAGATTTACAGAGATATAAATTGTATACTAAAGTTTTAATACATGTATAATGATAAAATTGATTAAAATACAAATAGTTTTAGCTTGATTTATACAATATCAAAGCATCCAATGTTCTTCTTTTAGTTCCAAGTATAACCAGTCATCAATCCCATCGATCGATTTTGTTACCGCCTTGCTCCTTAGTCATTGCATCATTCTCATTGCTAGCCAACTGAATATTAAAAGTGGATACTGCAGTATAAGATACATTCATATTTCTTTCAGGCACTGCCCAACGATGGATTACCCAACGTGGTTTGTGGCGACTGCAGACAAAAATTGGATTCCTTCGAAAAATTTCGAACCATGGCGCACAATTCACAAATAGCTTTGAAAGAATTTTTAAATATATCCAAAAATCTGAGACCAGTAAGTATAAGTATTGATTGGAAGGAACTTAGTTAGTCATACGCCATTAAATTAAATGACCATTTGAATTACTACTCACGAACTATGTTTTTTGCAGGACCCAAATGATTTGGAAACCAAGCTCGATGCAATTTTAAAGGCTTCATCGGAGGCTATAGCGGCAAAGG
 
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