CR60300

CRIMIC CR60300

Gene spz3

CG7104

Information

Donor Location Cytology FBti Stock number
pM37_p1 2L :..Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK OK TAAACAGCGCACAAGCAACGAG TACCATTTCCACTCGGGATTTCCC

Images

Construct Design Sequences

sgRNA sequence GAGTGCACTTGCAGTTGCAATGG
sgRNA2 sequence
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
 
Amplicon 5' Amplicon 3'
GCAACTAACAGGCTGGCACGCATCACGATTTATGTTGTGCCGGGGTGTATCTGTATCTAAGACCGTCTATCTAACGGCTCAAGTATCTACGTATCTTCGTATCTGAATCTGCTGCCCTTGTTGTCCTCTTGGAAAACTGTCAGCGGCGCTTCACTTTTTCGGATGTTTCACTTTCGGTCTAGCTTGTGGCGCCTCTTTGTTTCTGCCTCTCCATCTCCATCTCAATCTGAATCTCAATCTGCGAGTTGTCGAGGCATTAGCCAGCCAGCAACCTGCCAGACTTGAGACCTCAAGTACTCAACTCGGTTACGGGGCTATTATGCAAGAAGTCGCGTTTCAATCTCCCGAAATGAGGGGCAAGGGGCCGAGCTCATGAACCAGCTCTCATATGAAGACGTGCGCACGCAAGACCAAAACTCAGATACTAGTTTGGGTCGCTCGATGTCTTGGCGCGGCACTCAAGTACTCAACACTGGGTTTTCACCTTTGTGCAGGCAGAAAAATATCATAAAAGATGCGATCTGAACCAAAGATCAGTGCACAGAAACAAAGAATCTATTTCACAACCTGCCAAAGAATATATCTCATTAGCTGAAAGTGACATGAGATCTGAACACTGCGAGAAAAAAGTATAGAAATCAAGAAAAACGTCATAACATAATAACTAGGATATTTCTTAAATATTATTTTAAATCTGACAAATTTGTTTGGTAAGACATTTTTCCGGTGAGAGCTATAAATTACCTAAATAATTCAAAAGTCATAGCATAATAATAAGGAAACCACTTGTCTAGAACCCTTCAATTCCATTACATTTAGTTTAAGTAACATTTTGAGGAAATAAGTTTTTGCACTGTATGCTCCCTTTTTTGGATTCTGCAACCCGTTTTGCATCCTGCAGTATGGCTATAAAAGGGAAAGTTTCTGTCTGCGCCCAGATCCGGATCTGGGCATTACAAATGCCAGGCAGACATGAAATTTATACATGCAAGTGTCAACTGCAGTGC GTGACAACGGCCACGGATACAGCAATTCCCCCGTCCTTGCCATGAATCACCGCCGAAACAGCAGCCATTTAAAAGCACTCCAATTTCCATTTTCACTTCATTTTCGAATCCATTTACATTCCGTTCACAGATAAGTCGAATAACAATCAGATGCCCACAGTGACGCCCATCCAGGGACCGATTTACTTGAAGAACGGGACGGTTCCAGTGGTGCCGCTCTTCTCCTATCCGAAACTCAACAACGGATCGTTCCTACAGATTCCGGTAAGTACGGATCGATAGGTTCGCCGGCATGAGGTTCTAAGCCCGCGCGCACTTTAATTGACACACTGCGGAAAATATAGATCGCAAATAAAAGTAGGAGTCAAAAATCATTTATGAATTTTAAGTAACAAGCAGGCATCACCTATTCTTCCCATTTCTGTTTATATTGAAAATAATAAAAATTTGATTTGGTCTTCGGTGAGTTCTAGTTTTCCCGATTTCCTACAGTGTACTCTCGATATCGATCGAGTTTGTTCATTTGCGGAAATAACTTTGACGATTTTGTGTTTCATTTCGGTTTTATTTTCCTTTTTTTTTTGTTTTTTTGCGCACGAAGGTCGTGCGAGTTCGTTTCATTAAGAGGTGTGATGATGTTGATGACGACGAAAGAAGAGAGAGATTGATATTGAAAATCTTCGCATGGCGACTCGAGTAGGCTTAATCCCAAAATCCAACTCTAAACTGCACCGCCCGCCACGGCTACACCCTTCATATCTTCTTATTTTCGAATATAAAACGAACTTTTATGTTTATGACTTACTTGAGTCATTTTCACACCCCGGCCATAATTTGTTATGGTCGCCATTCCAATCTGAGGCTCAGTACCCCGCCGAAACTCAATTGCCGTCTTCAATGCTAAATAGTTAATACTTATCGCCCGGACTGACCATCGTATATCTCTGGTTAATTTAGATCTGGTGGACGGCTTTGTCGGTGGCTTTGGGACTGGATGTGCGCGGCGATGTCATCAAGGGAGTGCCG
 
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