CR60301

CRIMIC CR60301

Gene IntS2

CG8211

Information

Donor Location Cytology FBti Stock number
pM37_p0 X:..FBti0219221 Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK OK TGGCCACACTGACGTGTGTTGATT TGGAGCAGATACTTCAGTGGCAGT

Images

Construct Design Sequences

sgRNA sequence TAAGCAGAACTCCAAATGAATGG
sgRNA2 sequence
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
 
Amplicon 5' Amplicon 3'
GCGGATCGCAGTGGATTCTCGGATTCTCCGGCATTCCCTGCCCCAACTACAGATCGCCGAGATTAAAGCAGACCCGCGCCGGCATCTCCAGCACGGACACCCCGCCGGCCAGGCCAATAGAGCGGGACAACCGAGCACACGAGCATTGCCGGGTGCAACAAGTTGGAACGCAACGACAACGACGACGAGGACAAAATGCCGGTGAGGATGTACGATGTATCGCCGCGCGTTTTCTGCGCCATGCAGAATCTGGACATCACCCTGCTGGCCAGCTATCCGGAGGCGGAGATTCGGCCCGTGCTGCCCTCGCTGGTGCGGATGAGCCTGCTATCGCCACTGGACAACACCGAATCGTCGATGGAGTCGCGCAAAGAGATCCTGGCCGTGCTCATCGGCATCGAGGTGGTGAACAGCATCGTCTCCTACCTGCAAGTCAACTACCATGAGCTGGAGAATGAGCTGAAGAAGGAGCTGCAGGCCCGCCAGAAGTCGGCCTTCTTCGAGGGACAGCAACACGAGTACGGCCTCCAGTCGGGCATCGCACTGGGCTTTGAGCGGGCGGATGTGGCGCGCAAGGTGCGCGTGGTGCTCTCCGAGATCTTCAATCTGCAGCAGCAGGTCTCCGAGCAGAAACCGGCCGCCCATTCGGAGATGCTGGACGACGGCATCTATCTGGAGGAGGTGGTCGACATTCTGTGCATTGCTCTCGCCGAGCTGCCCTCCCTGCTGAACATACTGGAGCTAACGGACGCCCTTGTCCATGTGCCCAACGGACATCGCATCATCTGCGCACTGGTCGCCAACTTTCCGGATTGCTATCGCGACGTTGTGTCCCATGTGATCGCCAATTGCGACGAGGATGGCAGCGATGGCAAGCACCGGTTAATGCTGCTCATGGGGCTCAGCGAGATGAATCCCTCCCAGGCGCTGGCCAACAGGTCCATGTGCGTGGATATGCTGAAGGTGCCGTCGTTTATGCTTAAGCTCACGCTCAAGCATCCCGAGGATCTGGTGAGTAGG CAACCACTCCCCACAGATAGCCTTCCTCACGGGCCTGCTGCTGGGCAATGACCAGAATCTGCGCTCCTGGTTCGCCGCTTACATCCGCTCCAGCCAGAAGCGGAAGGGGGACGCACTGAATCTGGTGCGCGTGGAGCTGCTACAGAAGGTGATTCAAACGACGACCAATGCGGCGGAGCTGCGCGACTTCAATCTGCAGGGCGCGGTGCTGCTGCGTCTGTACTGTGCCCTGCGCGGCATCGGTGGCCTGAAGTTCAACGACGACGAGATCAATGCTCTGTCGCAGCTGGTCACGAGTTGTCCGCAGGCGACGCCCTCGGGCGTGAGATTTGTAACGCTGGCGCTGTGCATGCTCATCGCCTGTCCCTCGCTGGTGTCCACCATTCCACTGGAGAACAAGGCTGTGGAGTGGCTGCAGTGGCTCATACGGGAGGATGCCTTTTTCTGCAAGCGCCCCGGTACTAGCACCTCGCTGGGTGAGATGCTCCTGCTTCTGGCCATTCACTTTCACAGCAATCAGATCTCGGCCATCAGCGAGATGGTCTGCTCCACGCTGGCCATGAAGATACCCATCCGGCCGAACAGTACAAACAGAATCAAGCAGCTCTTCACACAGGACCTGTTCACGGAGCAGGTGGTGGCGCTGCATGCGGTTCGCGTACCCGTCACTCCGAATTTAAATGGCACCATACTGTGCTACCTGCCGGTGCACTGCATCCAGCAACTGCTGAAATCGAGGACATTTCTCAAGCACAAAGTGCCCATTAAGTCGTGGATCTTCAAGCAAATATGCAGTTCGGTGAGGCCAGTGCATCCTGTGATGCCGGCCCTGGTGGAGGTCTTTGTCAACACGCTGATAATACCCAATCCCACCGGAAAAGTGAATATTGATCACATGCACCGGCCCTTCACTGAGGCGGAAATTCTGCACGTGTTCCGCACATCCAAGCTCACATTCTTCGCCGAGGAGCTGCCACCGATGGCGGAAAGTCAGGAGCTCAACCAGATCGAGGTGACGTGTCCACTAACCGCCCAGCTGCTGATGATCTACTACCTCATGTTGTACGAGGATACGCGACTGATGAACCTCAGCGCTTTGGGTGGTCGCAAGCAGAAGGAGTACTCCAACAACTTCCTGGGCG
 
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