CR60304

CRIMIC CR60304

Gene CG7556

CG7556

Information

Donor Location Cytology FBti Stock number
pM37_p2 X:..FBti0219223 Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK OK AGTCTGCTGACGTTGCCACTTT GGTAGCCATTCTCCTTCATCTTGG

Images

Construct Design Sequences

sgRNA sequence CTGCCAAAACTCCGAGCACAAGG
sgRNA2 sequence
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
 
Amplicon 5' Amplicon 3'
GTATTTGTGGTGCGTGCGTGGGTCTGGCGCATTAAATTTGGATTTTACTCCGATTTGGACCTGACCTGGATTCGGAACTCGGACCACGATGATGATGCGACCGGAGCTGACGCTGGCCGGCCTCCTGCTGCTCTGCGCCACTGGCGCCAGCGCCTGGCACTCGGAGGAACTGGAGATCTTTGACTTGGTCGAGGAGGTAAACCGCAACTTCTACGAGTTCATGGGCATCAACCAAACGGCAACGGGCGCCGAGGTGAAGCGCGCCTTCCGCACCCTGTCCATTGTCCTGCATCCGGACAAGAATCCGGCCGAGGATGCCAACATCCAGTTCCGCAACCTCGTTTCCATCTACGAGGTTCTCAAAGATCCGTCGCGCCGAGAGAAGTACGACCGGGTACTGAAGGAGGGCATGCCCAACTGGAAGTCTGCCCTCTACTATTACCGACGTATGCGCAAGATCGGGCTCTATGAGGGCGCCTTTATCCTCTTCCTGATAACCACCGTGGGCCAGTATCTATTCGCCTGGGCGGCCTACCTCGAGAAGAAGTACACCGCCGAACAGGTCTTTGGCACCAAGCTGAAGAAGCTGCAGAAGAAGAACAAGAACATCGACATGGACGTGATCCTTAGCGAGATACCGATGCCCTCGCTACTCAACACCCTGCCCATCCAGATCCCCTTGGCCCTGTGGAACCTGCCGCGCACCATCAAGAACGGATTCAGCAAGGCCAACGAGCTAAAGGAACTCGCCCTGGAGAAGCGCAGGCAGTAAGTGGCAGGGTTCTATTGCTTACCTTACGTTTTTACGTTATTATAGCGCCTTGTTCCATTGTAGTCATTTTGGAGTTGTAGGATTTTGCTATTTCATTAAGTTTCTATATCGAGAGGTCCTGGCAGCGGAAGGATTATTATTTTGCGACCTTGT GCTCGGAGTTTTGGCAGACCTCTCTTACTTTAGTCTTCAATATCTTAGTATATAGAGCAACAAAAGAATATAATTCTAGTTTTGTATTGCATCAGGTTTTGATAACTTACATTGCCACTCTTATCGTTAATTCGCTGTGGTTTCTGTATGTTCATTGCATGCAGAGTGATATCATTTAATATTAAAAGTTGGTTAGTTTCTCATTTCTCAGTTGAAATTTTGGTCATAGCCTTTAGTTAGCATTTTATATTCACAAGCTAGAACAATTCTTCTAAATATAGGCAATTCAATTAAGAAACAAAGAAAGAAAACGATATGTCACCATGCAAACACATTTTATGCTTTCCAAAAAAGGTGCTCATGAGTGCACCTTAAGATGACTTGCACCATTAGATAGGTTCCAAAGAAGGAATATTGTCAGTCTTTGTGTCGCACGATAATCAAATGAAACTGAGCATTCTTGATATGACCACAGATAAGGTACTAAAATTCGATTAGCGTAGAATAGCTAACAAGCTAACAAAAATCGTGTGGGAACGGTATCCAAAAGTAAGAAGTAAGAAACTAATTGATTCATCCCAATTTGCATCCACAGGGAACTGGAGGCCGTCCGTCGTCAGGAGGAACTGGAACGGGAGGCCGAGGAGCAGGCGCGCCTTCGCAAGGAGCACAAGGAGAATCTGCGGAAGCGCAAGCAGAACAGCAAGGCTCCAGAGAAGACCGAGGAAGAATTGCGCGGCTATTCGCAGATTCAGACTCGCGAATTGACAGATGACGATGCAGTTCGTCCAGCTTCCCAGAAGGTATAAAGCCGACAGTATAGTGTTTCCTGTTCCCTAGTAAATTCGAAACCCTACTAATTTCATGCTTTTACTTTTAGAGCACGGTGAGCGGCGGCTTCTGGACTGACGAGGATCTTACCGAGCTGATTCGCTTGGTAAAAAAGTATCCTGGCGGAGCTGGCAGCCGCTGGAACACTATCGCCGAGTCGATGAACCGCAGCGTTCAGGAGGTGACCTTTATGGCG
 
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