CR60305

CRIMIC CR60305

Gene Inx5

CG7537

Information

Donor Location Cytology FBti Stock number
pM37_p0 X:..FBti0219224 Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK OK CCCAACTGACATACTGCCAAGTTC AGGGCACGAATTAACGAACGACAG

Images

Construct Design Sequences

sgRNA sequence CTATATTCAAAGAAAACCCACGG
sgRNA2 sequence
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
 
Amplicon 5' Amplicon 3'
CTGTGAACTGGCTACCATCCTCGGTCGGCAATTCGATTGCATTCATTGTCATCCCTCAAAGATCGAAGTGATTAATCCCAGGGGCAGCATAGATAGGGCAAAAACCCGGACGAAAACCAGGACAAAGGAGGATCGAAATCGGGACTGTGATCGGGAACAGGAACACGAACGGGAACAGGAACAGGAACATAAACAGGTAGCACCGCGAGGCGAAGAGGAGCGGAGCGTTGAGAGTTGGAAACTGTCCTCGAGGAACTCTTCCCGGAGGCAGAAGAAATGTTTTCGGCGGTGAAGCCGCTCTCCAAGTATCTCCAGTTCAAGTCAATACGCATCTACGATTCCGTCTTCACCATCCACTCCAGGTGCACCGTCGTCATCCTGCTCACCTGCTCGCTTCTCCTGTCCGCGCGTCAGTACTTTGGGGATCCGATCCAGTGCATCAGCGAGGAGAAGAACATCGAGTACATACAGTCCTACTGCTGGACCATGGGCACCTACATCCTCAAGCTGGACGACTTCGGCGATCAGGAGCAGGCGCTGGTTTCCCCAAATCAGGAAGTCTCCTACAACTCTGCCTTCTTCTCATCCGCCACAACCAACGCACCGCAGTCATCGTCGAGGGTCCGCACCAGGCCCCACTTCAGATCGAGTCTGCGCAGGATTGGCGAGTACAACGAGGCCTACGCGCGGTCCCTGTCGATAGCCGAGGGCGTGGGCCCCGAGATTCGTGGCCAGACGGAGCGGCAGTATCTGCGCTACTACCAGTGGGTCATCATCCTGCTGCTCTTCCAGTCGTTCGTCTTCTACTTCCCCTCGTGCCTGTGGAAGGTGTGGGAGGGCCGGCGGCTTAAGCAGCTCTGCTCGGAGGTCGGCGACGCGCTCCTCTCGGAGGAGACCTACAATACCCGGCTGCGCATGCTGGTCAAGTACTTCACCACCGACTACGAGGACATGCACTTTTGCTACATGGCCAAGTACGTCTTCTGCGAGGTCCTCAATTTCCTCATCAGTGTGAGTAGGTTTGTGAATGTGAAGAGGAG GTTTTCTTTGAATATAGAGCTCTTAGCTTGTGTAAAATATAAATTTAGTACTTAAACATAAGGACTTAACATAACAGTCAATAGGACATCTAAACTTTTGGATTTTGTATTATAGTTTTTTTGTTATCGAAAAACTAATATATCACAAGATATGTTTACTAATTTTACCATAATCGTAATACAGCTTATGGTATCCAATCCGCAGGTGGTGAACATAATCGTGTTGGAAGTGTTCCTGAACGGATTCTGGTCGAAGTATCTGCGCGCGCTGGCCACCATTCCGTTCTACGACTGGGATCGCTGGAACCGCGTGTCCTCGAGCGTCTTCCCGAAGATCGCCAAGTGCGAGGTGCTCAAGTTCGGGGGCAGCGGCACGGCGAACGTTATGGACAACCTGTGCATCCTGCCGCTGAACATTCTGAACGAGAAAATCTTCGTCTTCCTTTGGGCGTGGTTCCTGCTCATGGCCCTGATGTCCGGTTTGAACCTTCTGTGCCGGCTGGCCATGATTTGCAGCAGGTATCTCCGAGAGCAGATGATACGCAGCCAGTTGCGTTTCATGACCAAACGGCATGTGAAGCGTGCCCTGCGGGACCTGACCATCGGCGACTGGTTCCTGATGATGAAGGTTAGCGTCAACGTAAATCCTATGCTCTTCCGCGACCTAATGCAGGAACTTTGCGAGCTGCGCACCTCCGCATCGGGCAGCACCCTGGAGAGTCCCGTCTAGGATCGCTAGTGATCAGGTCCACGGATTGGATTAGGATCCCCAGTTCGCCTCGTCCTCCGACTGCCGACTATCAATTG
 
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