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CRIMIC CR60311

Gene Cpr47Ea

CG9079

Information

Donor	Location	Cytology	FBti	Stock number
pM37_p2	2R :..			Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TATTGCATCCAGTCGAGCATCCAG	AGCCTATATCTACTGCTACTGCGG

Images

Construct Design Sequences

sgRNA sequence	TTCATATGTGAGAAGCTCTTTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right

 

Amplicon 5'

Amplicon 3'

CGCATACGCTACGTGGTGCACTGGCAGCGACGGCAGCTCTTCCGATTCTGTTGCTGTCAGGCAGACAAACACTGTTTGTTTCGAATCCCGCTCGGCCTCCGCTCGTCGTCGGCTCCTCCTGCCCTTCCAGCCGCCAAATCTGCAAATCTGGGCTGCTGCACTGCATCTGGCTGCGGATGCTGCTTCTGCCGCTGCTCTGCTGTGAGCTGCAGTGAATGCGATTTCGGTATAAAAACGTTTGTGGCAACTGCAGCCAAGTCAAAGCACTTTTGACGAGCCTCCCAGCGCAGTACCCGCTATCAAGTCGCGAATCCGAAGTGAGTTTAAGTTCTAGTTGCGGTGGTGACTCCTTGAAGACAGGATCACACACAGATACATAGATCAGACACATAAGCTACAACTTGCGAGCGAGTGCAGAGACAAGTGGAGTTGATCCTGCATAAAGGATAACCAAAGTTTAAATTCAAAAGATCACAGTCATCTCCTGGATTTCAAGCCCCTTTTGGATATTTTTCCCACGACCTCCACCATCATAATCATCATAATTTCGCAGAACTTTCAAATCGCAAAAGCCAATCGGAGTATATCACATACTAATTGCCATGCAACGAGTTTGACATTCAAAGACAACGCCGGCGGCATCTCAATCGAGCGCCAAACACTGAAAACCATACGCCAAACACCAAACACCGCACCTCATCTGCAGTACTTACTTATGACTCTATTTAACCACCGTACCACCGCGCGGTTCCCCCCATCTTCTCTTTTGCCCACTAAAGGTCATGTCGCTCACCCACGCCTCCGCCGCAGCCCTCGTCTTGGCCCTCTGCTGCCTCAGTTTCATTCAGGCGCAGCCACAACGCGGACTTCCCCCACCACGCGGCAACTCCTTCGACGCGAACGCGGTGATTCTCAAGCAGAACTTCGACCTGAATCCCGATGGCTCCTATCAGTACAAGTAAGTTGGATCCAGGGATGGTAGGATCAATTTCAGGATTTGGAATGG

CTTGTAGCTACGAGACAAGCAACGGAATCCGAGCGGATGAGGCTGGCTATTTGAAGAACCCGGGCAGTCAGATCGAGGCTCAGGTTAGTGTGCTTTTCAGTGCAGCATTCAGCATATTGTAGCCTATTTTTCGGTGGTGCTGAAACTCTTTATTTCCCTGCACTTACAGGTGATGCAGGGCTCCTACTCGTACACCGGACCCGATGGCGTGGTCTACACCATCACCTACATTGCTGATGAGAACGGATACCGCGCCGAAGGAGCCCACATACCCACACCGCCACCAGTTCGCGCCGCCGCCGCTCCCGGAAGATTCTTCAAGTAATCCGCTCCGCTGTAGAATCGTGTTTTAATTCTTCATCGTTATGATCAAGTTGGCAATCATTTCGTTGTGGGTCAGTGCCGAACTGGCCGAAAGTGAACCAGAAACAGACAGGAGAGAGAGAGAGAGAGAGAGAGCAGCTGCTCGTTGGAGATCCTTTATCCCACCCATAATCATTTCCATTTTATTCCTGCCCCTGAAAAATCTCACTACCTTCGTGCTTCATCTGTGCATTTCTACAGCCAATTCAAATTCTACATTCTACTTAGTGCTTAACGAACAATATGTGAGATTATCATATGTAAATTACGTAAGATCGATTGTTAAATGTCGTACGGCAACGCATGCTCAGCAATAAAACAAAAATTGCACAAAAATCAATCCAAAGCCTTGTGGTCAAACCACATTTAGGAGAATGATCTCAGCATTGTGATGTAGGACATCGCCGTGCGAACAATCTAAAAACGGAAAGTTAGAAAAGAGTATTCATGTAAATCCATGTATTGTACAATGGAAATGATTCACCCACCGATGAGAAGGCCTCCATGTTTGCCTCGAAAAAGTATCCCGTAATGCGGAATCCCCGATGAGCCCGCATCATGCTGATCAGCAAGGATCGGCAGATCGAGGTAGAGGCTCCACCAGCACCCAAACTCTCGTGCCACGGACTGTCGTAGATGG

 

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