CR60312
CRIMIC CR60312
CG13900
Information
| Donor |
Location |
Cytology |
FBti |
Stock number |
| pM37_p1 | 3L:.. | | | Order from Bellen Lab |
PCR Verification
PCR check relative to sgRNA sequence
| HA-L |
HA-R |
Forward |
Reverse |
| OK |
OK |
TTGGTATGTGGAACTGGGATTGGC |
ATCGTCCGTTTCCAGGGTGATCTT |
Images
Construct Design Sequences
| sgRNA sequence |
CATAAACCAACTCAACGGAGTGG
|
| sgRNA2 sequence |
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| Amplicon PCR 5' Left |
Amplicon PCR 5' Right |
Amplicon PCR 3' Left |
Amplicon PCR 3' Right |
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| Amplicon 5' |
Amplicon 3' |
| GGCCGCGCACTATCGATAAGTCAAGCCACATTTTATGCACCACCATTTTTACTTTTATAGTTCTTTTACTTTTTGTCGGCGAACAGAGCAGATGTGAAAATGAAGAGTTCTGAGTAATCAGGAAAATTCGAATCCAAAGGCATCGCTGGTGAAATGTGCTAATTCATCCATTTAACCAGCCGAAGGTAAACAAATACACGTGCTAGCACTGATATCTACTATTATTTAAGCGCTTTCCGCACGAAGTATCTCCAGAAATCAGAAATGTCTGTGCTTTGGTCGCATTTGAAGCGTGCTAAACTAAGAATTAAATGCTGCTTACTAGCATCGTTAGGCGACACGACTTTCCATACTGTGGCCATGGTTTTCAAAGTAGTACAAATTCACAAAAAAAAAAATACACCGCCATTCACGTGACTTTTTCCCTTGTCTGCAACTGTACCTAACCTCAACATTGCTTCTCTTGCAGTCGTGGACAAAAGTGAAAGAGTGTAGTGCTCCACCATGTATCTGTACAACCTGACCCTGCAGAAGGCCACCGGCGTGACCCACGCGGTCCACGGCAACTTCTCCGGCGGCAAGCAGCAGGAGGTCCTCCTGTCGCGTGGCAAGTCGCTGGAGCTACTGCGTCCGGACTCCAACACCGGCAAGGTGCACACCCTGCTCTCCACGGAGATATTCGGATGCGTTCGCGCCCTGATGGCCTTTCGACTGACGGGCGGCACTAAAGGTGAGTCTTTCAATTGTAATTTAGTTTTGTACCATCAATTGGGAGTAATGTATTGCTCCTATATAATCTATGTAAGTCGCACAAATCCCTATTGAGGCAACAGACGGCTAGTTGACCCCAGTAAGACCTTTGAACGCGTCTAAGTGCGAGAGAAACCGAAAGACTTGACGTCGCATTTGCCATAAACCAACTCAACG
|
GAGTGGCGAATTGAAACTTCGAGTTACAAAGTCAATAAAATATCATTATAGTCATTAGAAAGTGTTAAATGTATGACCCTAATCTCGAATATCGTTACCTTGCTTTGCAGATTACATAGTTGTGGGCTCGGACTCGGGACGCATCGTGATCCTCGAGTACAATCCCTCCAAGAATGCTCTTGAAAAAGTGCACCAGGAGACGTTCGGAAAGTCGGGATGTCGCAGGATTGTGCCTGGGCAATACTTTGCCATCGATCCTAAAGGAAGAGCCGTGATGATTGGCGCCGTGGAGAAGCAAAAGCTGGCCTACATCATGAACCGGGACACGCAGGCTCGCCTCACGATCTCATCGCCCCTGGAGGCCCACAAATCGAATACACTGACATACCACATGGTCGGCGTGGATGTGGGCTTCGATAATCCCATGTTCGCCTGCCTAGAGATCGACTACGAAGAGGCCGACATGGATCCATCGGGTAAGCATCAACTAGGTGGCGTGTAGACCCTAGAAGGCGCTTTAATTCACAGGTTTAGTGGATCCACACCACGAACACTGACTGTACTTAGTGCCAGATAAATCGTGATCACTGAACCATTGGTGTTGCTGTAATTGCCTAGCCACACTTCACTGATTTGTGACCCACACTTCCTAATCTCAATTGATAACATTTTGATAGTACAATGCTATAGTCCTGTAACTAATCAGTGTCGCATCTATTTGTTCCAGGTGACGCCGCCCAGCGCACTCAGCAAACGCTGACTTTCTACGAGTTGGATCTCGGCTTAAACCACGTGGTTCGCAAATACTCGGAGCCCTTGGAAGAGCACGCCAATTTCTTGGTATCCGTGCCTGGGGGCAACGATGGTCCTTCGGGCGTGCTGATCTGCTCGGAGAACTACCTAACGTACAAGAATCTCGGCGATCAGCACGATATCCGCTGTCCCATTCCGCGCAGGAGAAACGATCTGGATGATCCCGAACGTGGTATGATCTTCATCTGCTCGGCCACACATCGCACCAAGAGCATGTACTTCTTCCTGCTG
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