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CRIMIC CR60314

Gene CG12910

CG12910

Information

Donor	Location	Cytology	FBti	Stock number
pM37_p1	2R :..			Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CATTAGTTCATGTCACCATTGGAAATC	GTTGAAGGTGTCGTTACTGTCTCG

Images

Construct Design Sequences

sgRNA sequence	GTAGAATGGAATGGATGGGCAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right

 

Amplicon 5'

Amplicon 3'

GACCATTTGGCACATCAGTTGTGATACAGACTAAGATATCTTTTTATATGAAAACCATTTCAAAGTGGACTTTTGGGGATTAAGACCATATAATTTATCGCTCTGCCAGGCCGAGTGCTCTTCTTTCTGCCAGTGTGTGTGAGCCATAATTATCTGCGTATTGTGGGGCGCCAAGTGAACTTGTTGGCTGGGTCGGTCAGTGGTTGGTGCCAAGGTGGCGTGCGGGTGTGGTGGTGGCCAAAGAACGCCGAGGACGGGCGGCAGTCGATGGTGATGACACTGGAGGGACGGCCGCACAGAGTGGCAGCAACAACCACACACGGGCTATCGCTAATCGATGGCCGGCCATCAAGAGCGCTAAGCAAATAAGCAACAGGAGCCGCTTGGGGTTGACTGCAGACAGAAAGCCGGGGCCCCAAGTCCGAGAATCAGAAGACCCGAGACCCAAGACGTCGGCTGCTCAGTCGACGGTAACGACAAGCCACGCGCCGCGCAGCTAGCAAATAAATAAACAGTTTTAGTTGCCGTCGGCGATTCCTGAGGAGCGGAGTCCCTGCCGCACAGTTTGTTCCCGCTCGAGACATGCTGGCCGCCCCGGAGAACCGCTTCCAGCATGTGTACTTCGCGCTATCCGCCCTGGTGGCTAGCGCCATGCTCGTCCTGGTCTACATATCCATGCGCTCGAGCCTTGTGAGTGCAGCGGAATGCCCTTTGTCCTTGCAACGATCCAGTGACTTATGCCCTGAACTCTTTTTCTTGCAGAACGAGCGGCTGCGCGAGGATCTGGCCAAGGCGCAGCGACAGATGATCTTCGAGAGGCCGAATCCACACTGGGACTACAGCAACAGTTGGCGCCGGATAGGGAACAGCACGCTCAGGCATGAGATATACTCGGCGTATTTCGATGCCAGGACGGATATCGTAGGTGAGATTGTCCGAAGGATAGAACATATACAGAGCTGCACCTAGGATTGTTATACCTCTTCAATATATTTACATTTTGAAGTCTTAAATCCTGTAATTTTGGTCAGTTCTGTTATACTTCATAGAACAAATTAAATTTATATATGCTCATGAGCGCCTGCC

CATCCATTCCATTCTACACAAAGTATAAATACATAATAAACAACAAAGAAATGTTGGCCTTTTATATTTGATTATATATATTTTGTACGCATTACTTTTATACTGCTATAAAAACGATCTGCCAATTTAAATGACATAAATATGAAAATCTTTAAATATAGATTCGTAGATACAACACTTTCCAAAAGCTATAAGTAAACATTTCATTTGGTTAGCTATGACTTCATAACGGTCCCATTAGGACTGAAGAACAATTATCATATCAATCAATTTTAAATAACTTTAAATTATTATTCTTTTCTATTCCAACAGGTAATGTCCGCATGGATGACGAGCAGATGACAATTGGTTCGCTGCGCATTTTCGCCATTCTGCCGGAGCGTCTGCGCGACTCGTCGATAAACTGCATCGTGCGCTTCTCGGACTTCTCCAGCCAGGAGATCAAGGCCGAGGAGGCGGGCGCCATGCACGATGTGCACAACAACACCTTTGCGGCGTGGAGCATCATGTGTCCGCTGCACGCCTCCCGCCGCTCTCCCTTGCGCCTTCCCCAGGCAGTGGCCTTGGCCTACGCGTCCAATCGACTCAGTCACCTTAGTCCTACCTTTATTCAGATCAGGTGAGTCATCTATCCACCCAACCAAGATGATCTTAAATAATCATATTATCTAATATTAGACTGCAAGTCGCTCTTTAACCTCGTTCTTAATGCATTTCCACAGTTATCCCCGCAACATGACCAACATGTTTGGCCGATCTCGACCCACGATCTCCGTGTGTGTGGGTCCCCTCCACGAGAACTACTCGAATGTGCTGCGGCTGGTGGAGTTCGTGGAGATGTACCGCCTGCAGGGTGCAACCCACTTCTACTTCTACTACGTAGAGGCCAGCGAGGAGGTGCTTCGCGTGCTGGAGCACTACCAACGGATTGGCCTGGCCGATGTCTTCGAATGGAATGTCCAAGCGCATATGCAGGATGTCCACTACGCCGGAATCGTGGCGCAGTTCAACGACTGTGTGTACCGTGCCAATGTGGTGGACAACTTTCGATACGCGGCAG

 

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