CR60315
CRIMIC CR60315
CG13424
Information
| Donor |
Location |
Cytology |
FBti |
Stock number |
| pM37_p1
| 2R
:.. | | | 94375
|
PCR Verification
PCR check relative to sgRNA sequence
| HA-L |
HA-R |
Forward |
Reverse |
| OK (weak) |
OK |
GCGTTACAATCACTTAGACTAATGGC |
AAAGGCGATCACCAACCACCAT |
Images
Construct Design Sequences
| sgRNA sequence |
AGCACTGACTATTTGGCTGTTGG
|
| sgRNA2 sequence |
|
| Amplicon PCR 5' Left |
Amplicon PCR 5' Right |
Amplicon PCR 3' Left |
Amplicon PCR 3' Right |
|
|
|
|
| Amplicon 5' |
Amplicon 3' |
| CTGAATTAACCCAAAAGAGGACACGTGCCTGCACACCAAAGCACTGAAAACAAATCCAGTCAAAGAAATTGCTCGCCGTTCATTAATCAAATGGATGAAATATGTTGGCAAACGTATTAAATATATTTAGCAAAGCCATCGAAATATTAAATAGCGCTACTTGTAGACAATTAGCCAAAAATTATGACTTTTAATCACGGGCAAGGCGACAAAAACAATTTCGAAAACCCAGAAGACGGCGTTGAACGCGAGCCACGCCCCTCATCCCAAACATTTCACACATGTTTTAAAAATAACCGAACTTGTGTGCTGTCTTCAGTGAAAATCGAATAGTATGTTTCTGTTCCTTTCTGTCGTTCTATTTGCGCATATGGTGTGAGCGGTGCGGAGATATATGATTTTAGGCTCCGCCCCGCCAGGCGATCATCTTGAAAAATCGGCCAGTGAGCAGGATGCTCCGCATATTCAAATTAGTTTTCCGTAAGATCTCCACGCGCGCTGTTGTCTCCGGAATATCTTCAAAATATATATCGCTCGCAATCGGCGGAAACATGAATTCGCAATCGGCAGTGCGTCGCTATCCACACAGTTTTTCCATCGAACAAATTCTGGCCAAGCCGGAAATGCGCAGCTCCACTTCCTTTGAGGACTCCGTGCAGGATGAAAGCGGTCGCGGTGGCAATTGTCTCGGTGCTTCACGGGCTTCCAGTCCGGCAACATCGAGTTGCTTGGATGACAACATGGACGACGGCAAAAGTGATATCGATTTGGCCTCAGATGATGGAAACGGTGCGTATGATCGATATGCCGGAATATAAGCTTTACAACGACGAGAATTTTGGTGTCTGGAATTGGATTTGGGATCTAAAAACTTAACTTAAAGCTCAATTGGATCCTTATTAAATAGCAAATATACAAAATATAGATTTATACTGAACTTCATCAGGATAAATAGCACCAAC
|
GCCAAATAGTCAGTGCTTTAGTGCTTTTAAGTGTAGTATTATCAAGGTTCAAGGAATTATCAAGGATCTTTCAAATAATACAGTTACAATATACAAATTTTAAATTCTGAAAAAAAATGTCTCTAAAAGATGATTTACCTCCTAATGGAATCACCCATAACGAGCTCAGCACAGCCGTTCATTATTGTATGTAAATATGCAATGAGTTTCCCACCTGAACGAATGAGTGAGTGCCTATGGCTGAATGATCTCATCTGTCATCCGTGCGATCAAGAAACAAATGATCAGAAATTAGCTTCTGGGTGACAGGGAATAAGCCATCCATTCCATATCCACATTTTCGTCGACACCAACGCAGTCGACTAATGATGCAAATGTCACAAGCGAACAAAGATCGCTGGCGATCTCAAAGGAGATATTCTTCCACCCGTTAAGAAGGAATTTATATGGCTATTTGTTTTCGGTCGGCCAAGAGAATGTATGCCAAAATGGCCAATTAGACGCCTTGGCTAAGTGAAATGCAACAGTGTGATTTGCAATTTGCCAGGGGTTTTGCGGTGCGACGGGGCATTTTACATACACATACAACTGTGCCAACAACGGATCGACAAATTACCATTAGGCGAAAATAGTTGGCACCGCGCGCCGGATTGCCCGCTGTCGGTAGCAATAAATGTCTAATTGGGATTTCGATTTCCCACTTTGATTCCATTGCAGGCCTTGGTGATGACCGCAAAAAGCGACCGCGTACCGCCTTCTCGGCAGCTCAAATCAAAGCACTGGAAACGGAGTTCGAAAGGGGAAAGTATCTTTCGGTGGCGAAGCGAACTGCACTGGCCAAGCAGCTGCAGCTCACAGAAACGCAGGTTAGTTGTGGAATCTATCAAAAAATTTAATTACTACCCAAAAAAGTTTGCCATTCTATCTAAAAGCGATTAATAACATGAAATGAAATCCCTTGTATCTATGAGATACAATTGGCATTCCTCGTAAATAATTCATTCATTCATTTCACTTTTCAGATCAAGATCTGGTTCCAAAACCGC
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