CR60324
CRIMIC CR60324
CG5392
Information
| Donor |
Location |
Cytology |
FBti |
Stock number |
| pM37_p1
| 3L:.. | | | Order from Bellen Lab |
PCR Verification
PCR check relative to sgRNA sequence
| HA-L |
HA-R |
Forward |
Reverse |
| OK |
OK |
GACTCGCTTGGCCGAAATGAAA |
TGTAGCAGACTGGGCTTTTGGTCT |
Images
Construct Design Sequences
| sgRNA sequence |
AGAGATATCTGTCAAAGGAGCGG
|
| sgRNA2 sequence |
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| Amplicon PCR 5' Left |
Amplicon PCR 5' Right |
Amplicon PCR 3' Left |
Amplicon PCR 3' Right |
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| Amplicon 5' |
Amplicon 3' |
| GCAATTGCACACTTAATTAATGGGCACTGCTTTTGATCTTCTGTGAAGTTCAGTAGCCCCCCAATTCCTGACGCAAGTGACAAAAACTGAAATTTTTCACGCACTCAGCGAAACTCTTTGAACCCGCCCTTTACGCCCAGCCAACTGTCACACGTCCAAGTGCCACGGATGCGGAGTTTCGAGTCCGGAGATCGGGGACATGTGTCGCAGATGTCAGACATGGGAAACGCTTCCACATAGTGATGGGCATCCCGACGTGATTTCAAAAAAGTCAAATACTTTTAAGTGATCTTCAAAGAAAAGATATTGCATTCGTGTCACGACTTACAGTTCTCTGCCAGCAAACTCATTCATATTCATCTGGTTGGGATATGCAGAGCTATCTCCTTGTTCAATCTGTGGCCCAATCAGAATAATGTCAATTATAGGTAGGTGAGACAAGCTGTTACAATTGACAGTGAATTGATCTTCAAGATCTTACAAGGAATTTAAATCAGATCGCTGGTTGAACTTTAACAAGCTAAAACTGGTAACTGGGTAATATATTTCATGTTTCAAATACCATAAAATATTTCTTTTTATATTAATTATGCATAATGCATGATTATCTTGTGAACAAAACTTTTTGTTATATTTTTGCCCATCCTATCACGCTACCATCCCTGATCCCAAAACCGAACAACTCATTGTGTCCCCCCCCTTTTTTTTCGCAAGAAAAGTTCAACACCCAATGAGCAATCCTCCAAATGAACCGCAGCCAGATAAAAAGGGGAACGGATAAAAAAAAAAAATTGGAAAGGAAAACAAGCCTTAAGTGCTTAATAAGTAATCAGTCACCCTGGGTACTCGTAGATAAGCAGGATACCATTCCAGCCACCTAATTAGTGCGACAAACGAAATCCCATCCCCGTGAAAGTCCCTGTGTGATTTGTGCCAGAGGAAGTGTATGTTGCAACAGATTCAGCACAACATGTGGCGATCATGATCATGGTCATGATCACAAGCGGGAATCAGGAATCGGGCATCAAGCGAATCTGCGTGCAATCCGCTC
|
CTTTGACAGATATCTCTCTGCCTTCCGTGGCATTTATTTAAATTATAATTTACTGAGTGGTGTCAAAATGCGTGAGCCAAGCCATTAAACCCTGATATGCTGCACTTTGCCAACTTAACTTCTTGTTTTCCACTCGCAACATGCTTATTTTCTGTTTGTTTGCTTCTCTTGCAGATATCTTCACATGTTGCAATCAGGTCTTCGGCTCATGTCGCACAGCATGCGAAAATGTAAGTATCGGCGATCAGAGGAAAGGCGGAAAAAAGGGGGTCTTAGTGGGTAGGATTGATCATATATCAAAAATGCGTATGAATGCGTATGAGTGGATGCAACGGGTGATACAGACAATGGCAGCACATTAATAACAAGCTCAAAAATCTCTTTTTTTTACTATTAACTTGGGATTCTTTTTTATATATTCTTGAGGTTATCATACGAATATTAAAAATTCTTTTTTCAAATATTTCCTTCGGCCTTTGCATAGATTTTCCATTCTAATACTTAAACTCATAACTATCTTAAGCCAAACCGATGTGGCAGGCGCCACTTGCACAGTGAAAACCAACTCGAAGGGAAATGATGTGTCTGTCATAAATATTAAACGTTGCTGCCCCACAGGCAAGTGGCCATAAGTATGAACACCTCGGACAGCAGATCAGATACAGATTGAACAAGCCGATAAGGTGGAAAAACTACTGATCAAGCCACAATGCATTTCCTGGTATTTTTATGTGCCCATGAATCCTCTTTTCCCTCGTTATGAATGGAGTACGAGCGGTGCATGCCAAGCGAAATAAATTTAGAATTCAATAAACGTTGATCCGTCGTTGGACTGTCACACGCAGAAAATGCATTGAAAATTATTATGACAGGTTTTAATGGTCGTTCGATTTCGGTGGAACTTTGAATACCATATCCACATCCGCTGGCAGCCGATTTCAGATTATGAAGCCTTGTGCCAAAAAGGATTGAAAAATCCGCACAAATTGCATTGTTAATTAGCATAAACTGGCAAACAGGTAATTGC
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