CR60327

CRIMIC CR60327

Gene hll

CG4500

Information

Donor Location Cytology FBti Stock number
pM37_p2 2L:..94379

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK multi band CCTGGATGAGCCATCATCCTTT ACTTGTACAGACGCTCGATGAC

Images

Construct Design Sequences

sgRNA sequence AACAAATGAATCAGCGTAAAAGG
sgRNA2 sequence
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
 
Amplicon 5' Amplicon 3'
CGCCTTGGTGTGGGAAACCCCAGGATCTGGGAACGATGGTTGGACCACTCTTACGTTTGGCGAGTACCAGGAGCGCGTGGAGCAAGCGGCGCTGATGCTGCTGAGCGTGGGAGTCGAGGAACGCAGTTCGGTGGGCATTCTGGCCTTCAATTGTCCCGAGTGGTTCTTCGCGGAATTTGGTGCTTTGAGAGCTGGAGCTGTTGTAGCCGGAGTTTATCCGAGCAATTCGGCTGAGGCCGTTCATCACGTCCTAGCCACAGGAGAGTCATCCGTTTGCGTAGTGGACGACGCCCAGCAGATGGCCAAGCTGAGGGCCATCAAGGAACGCCTGCCACGTCTTAAGGCTGTAATCCAGTTGCACGGACCATTTGAAGCCTTTGTCGATCACGAGCCCGGTTACTTTAGCTGGCAGAAACTCCAGGAGCAGACCTTCTCCAGCGAGCTGAAAGAGGAACTGCTGGCCCGTGAGAGCCGGATTCGTGCCAACGAGTGTGCCATGCTGATCTTCACCTCGGGCACAGTGGGTATGCCAAAGGCAGTGATGCTATCCCACGACAACCTCGTGTTCGACACAAAGTCCGCAGCCGCACACATGCAAGATATCCAAGTTGGCAAGGAGAGTTTTGTGAGCTACCTTCCGCTGAGCCATGTGGCTGCCCAGATTTTCGATGTGTTCCTGGGTCTTTCCCATGCCGGATGCGTCACATTTGCCGACAAGGACGCACTGAAGGGAACGCTGATCAAGACCTTTCGCAAGGCGAGGCCAACGAAAATGTTCGGCGTTCCGCGCGTCTTCGAGAAACTTCAAGAGCGTCTGGTGGCCGCGGAGGCCAAGGCGAGACCTTACTCCAGATTACTTCTAGCAAGAGCTAGAGCTGCTGTCGCTGAACACCAAACAACGCTGATGGCGGGGTAAGTAGTGATAATCCAAAGTAATACTATCAAAAAAGATTTAAGGTCAATGGCCAGACATAACAACGAATTTGGAATAGCC CGCTGATTCATTTGTTAGACTTAGAGATTATATAGCTTAGTAATTGATTAATTTAATTTTTATTTGTTTTTATAGGAAGTCCCCATCGATTTATGGCAACGCTAAATATTGGCTTGCGTGTCGAGTAGTAAAGCCAATACGTGAAATGATAGGTGTGGACAACTGCCGTGTTTTCTTCACCGGCGGAGCACCAACGTCGGAAGAGCTGAAGCAATTCTTCCTGGGACTGGACATCGCCTTGGGCGAGTGCTATGGAATGTCGGAGACTAGTGGTGCTATCACCTTGAATGTCGATATAAGCAATCTGTATAGCGCTGGTCAAGCGTGCGAGGGAGTCACTTTGAAGATCCACGAACCCGATTGCAATGGTCAGGGAGAGATTCTGATGCGGGGTCGCTTGGTTTTCATGGGCTACTTGGGACTGCCGGATAAGACTGAGGAAACGGTCAAAGAGGATGGCTGGCTGCATTCCGGTGACTTGGGCTACATCGATCCCAAGGGTAATCTAATTATATCCGGTCGACTCAAGGAGCTGATCATCACCGCTGGCGGGGAGAATATTCCACCTGTGCACATCGAGGAGCTGATCAAAAAGGAGCTTCCCTGCGTGAGCAATGTGCTACTTATCGGCGATCATCGAAAGTATCTCACCGTCCTGCTGTCCTTGAAGACGAAATGCGATGCAAAGACTGGAATTCCACTGGATGCTCTGCGTGAGGAGACAATCGAATGGCTGCGGGACCTGGATATCCATGAGACCCGTCTCTCCGAACTCCTAAACATTCCAGCCGATCTCCAGCTGCCCAATGATACTGCCGCTCTGGCAGCTACCTTGGAGATCACCGCCAAACCTAAGCTCCTGGAAGCCATCGAAGAGGGCATCAAGCGAGCGAATAAGTATGCCATTTCAAATGCTCAAAAGGTGCAAAAGTTTGCCCTCATTGCTCATGAGTTTTCCGTGGCCACCGGAGAGCTGGGTCCC
 

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