CR60336

CRIMIC CR60336

Gene CkIIalpha-i3

CG3217

Information

Donor Location Cytology FBti Stock number
pM37_p0 3L:..Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK multi band CAAATCTGGCTTGGGTATTGGC GGACCCTGTTGGTTATTCCACTCT

Images

Construct Design Sequences

sgRNA sequence AGTTTTCATGAAAACATGGATGG
sgRNA2 sequence
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
 
Amplicon 5' Amplicon 3'
CAACGCCGATCTGTAAGCAAGTTCGTCTTGGAAATTGACAAAAACCGAAAAATCTGGCAGCACCAATATGACACTAAAGGTTCAAGAAAAGACCTTCGGTTGCACAAAATAGAAAATTCAAACATAATTGAAAAGAATTAACTAGTATCGTGAAAACATGTAATACAGTTAATAAAAAGTTATAGTATTATGCAATATTGATCAAATGAAGCAAACTTTATCGATAACTTCGAAATTGCAACCCTAAATGCATCGGATGGGCAATACCAATCTGAAGTCTACAAAAAAGCATATTGTGGAAGTCTAAGTTTTGTTAAATTTATGGAAAAGGAATCCAATTTATCGGACAACTTGCAGTCCCGGTTATTTGCACTGGGTCCTGCCCCGCCAGGATTTCGTCCACCGGATGCTACCGCCTTTGCCACAGCGGAGTCAAATCTTCGTAATGCCATCCGCTACCTTAATGATACCTATGGCGAGCTCGATCAGCTGCGCACTGTCATCCAGAAGGAGAAGAATAACTCGAAGCGTACGTCCTACAAGGCGCAGTCCATGATGCTTGCCATGCAAGTGATACGTTTCAAAGGACTTATTGGGAAGTACAGGGACATTCTGATTGGCCTGCAGGATGCCGAAATCATCAAGGTCAAACAATACATAGAGCAAATGGTGGAGATGAGGAACAAAATCCAGGCAAAGACCGATCCCACACAGCGCGAGTTCCAAGTGGGCAGCTCAATCTCACTGGTGCAGGAACAGCGCAAGACCAAGAGAATCTGCCGCAGGTGGCGGGATCGCCTGGTCCAGCTGACGGAGGCCAAGATGGACAAAACTGAGCAACTTACTGAGAAACCAAAGCTGATCAATGTAAGAACCGTTACAAAAGAACTTATACATATTTTCATTGCGCTTCCAATATCCATCC GTTTTCATGAAAACTCTCATCGAATTCTGATCGAATTTCGACTGAACTCGATATGCTTTTTTAGAAACCTAGAAATATGTTAAATGCAAAAACCAACAAGTGGAAGGAAGCTGTAAGCTTGGTGAGTATAGCCAAACATATAAAGGATAAGTTAAATGCTAGTAAATATAAAACTAATCCTAGCAACAAAGCGGTCTAATTAAAAGTGGCTGTAAGAGGTGTGAATACCTAAGAAAGCTGACTAAGAATCGTCCATCCAAACTTTGTAAGGTGTGTAAAAAATGGTCTGATGATGAAAAAGCAAAAGCGGCAGGAAATAAGAAAGCCAACAAAACGCCAATAATGGTATGACACTATTGAAAAGTCGCAACATAAATTAATACTATTATTGCAGGCGAAGTCGAAAAAATTAGAAGTACGCGTGCGTACGGATTCTGAAGAAGCTATAATTAAAATAGAGATAGAGGTGGGTGTGTACAAGTAGATCAACAGTAACATTAGCACTTATTATTAATTTTCTTAGCATGGTGAATTTACCGATGAAGAGGAAATTGGGTCATTGGAAAAGGAAACGACCTTAAAAGATGAGATCAAGGAGGTTAGTTGTTTCTTCAACCTGAAAAATGTGTTTAATTTTGGAATGTTTTTTTTTAGACTGTTGAATCTCACGACACGGCAGCAGTTGAGGTAAGTAAACAGGTCCTTGGACTGTAATAGAACTAACGTAACGATAAATGACTCAATGACAGGAGGAAAATGAGACCAGTCAAGACGATGTGGACCCCAAAGTAGTCGCTTCCTTACAAAATTCACTGAAATCCATCGACTGTAAAAATATGGCCTTGGCCTACGTGAAGGTCTTACAGGAGTACGCCATGTTAAATGATAACTATCGGGCCATTGGACTACTTACTGAAGTTGAAAAATCAGTTTTAAATCCGCCTCAGAACGAGGATTTCGATCTATGATCTTATTGACGCCACAAAAATAAAGTTTAGTTTTTATATGTAATCTACAATTAAAATGTTCGTGTGTCCTGCCTTTGAGAGTCAAGACCCAAAATG
 

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