CR60368

CRIMIC CR60368

Gene CG6420

CG6420

Information

Donor Location Cytology FBti Stock number
pM37_p0 3R :..Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK OK

Images

Construct Design Sequences

sgRNA sequence GACACTGCATGCAAATGGCAAGG
sgRNA2 sequence
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
 
Amplicon 5' Amplicon 3'
GCCGGAAAACTCGGAAAACTTGGCGAACGCAGCTGCAACGGCAGAAACAACAACAGTGCAAGCACAGCCCCACGAAACTCGGTGTAGTGAAGGAATTAAATCTAATTATAAGAAGAAAACAAGTGAAGGGCAAGCCCAACCCCGCGGCGCTTAATTAGCGGCGACAGCCAGCGATTTTCGTGGCTGGGTAGGAGCAACTGCAGCGCCCGCAGAGCCACCACTAGCACCACTGGCACCTGGCATTCACATGGGCTGAGTGCCGAGTGGATCGGTGGATCGGCGAAGGCGCAGCAGCAGCAGAGGAGCAGAAGCGGGAGCCGCACCAGCGATGGCCAACCAACTGGACGCCAGTGTGAAGGACGACCTGAAGACGCAGTTCGTCACCCGCGAGGGCACCTACCGCCTGCTGACCCTCTCGGAATACTCGCGGCCCAACCGGGTGGGCTACAGCAGCAACCAGAGCTCGCCGCAGGTGCGCGTCTCCATGGTGACCCTGCCCAGTCCGGCGCAGGGCAAGCTCGGCTCGGATGTGGGCGTGGGCACACCTGTGGGCGGCGGAACAGCAGGAGCCAACACCACCACAACGAATGGCAGCTCGCCGGGAGCGTCGCCTACGGGAGCAGCCGGAGCCAGCACAGCAATATCGAATGGAGGCGCTGGCGGTGACTATAGCCACAGCAACCACAACAGCAACAGTGCCGGAAACAACACTGTGGAGGCGCGTCTGGGCGGCGGCATATCCATGCACTCCATGATGAACGGCGGCGTCGTCGACCAGAATGGCGTGGCCACCAATCAGGTCCTAGGAGGCGATCGCATATGCTTCAACTTCGGACGAGATCTCTACGTTTACTCCTTTCGCGGAGCCAAGAAGGTAAACATTGTCGGCCAGAGATAGTCTCCATGCGCAAGCACGTGCGATGGAGGAGAAATGGTGCAGGTGGCGGTGCAGCGAGCTTATCACACCAGAGATAGAATAGAGGCTGCCGGCAATTGCATTCCAGGCGGATTGCACCAGCCAATTGGCGAGGGCCTAAAATACCTTGC CCAGTGTGCTTAATTTGCTCCACTTCGAGCGCTTGATGATCAATTCGCTTGTTTGGCGTTTTTATGAATGTTTATTGCGCTCGAAGGCAGTCGGTTGTGAGCGGTTTCAGCTCATCCCTGCGCGCGGATTACCCTAACTTTTGTTTGCACCTGCTGTAGTCAACGAACATTCCGATAGAGTAATCCTGGTGCGGCTTTCGACCATCCGAATTGCTCATCGCGTCGCTTGTGTTTGCCCTGTTTCCCCTGCCCAGCAGCTGTTTCTAATTCTTTGGCATTCTGTGCGGCAACAGCTTGCGATTCTCCTTATGTATTTGCTTTAGCTGGCTTCTTTGTTGCCATAGTTATCTGCTGGCGAGAACTTGGCAAGGCTTGCATAGAGCTTTTCCCACCTGATTCCGCCAACAAGCTGTTAATTTCCACTCCGCTTTCCGAATGTGTTAATTTCCAATCTTATTTCAACCCACTGAACGTATTCTTCCATTCCAAACTTGGCTCAAACGAAATTATTTGAGCCTGTTCCGCTTTGCCATTTGACTTTCACTGCATTCATATTCTTAATCTTGAAAATTATTCTATGATGATGACTTATATTTCAGTGATTTGTTTATACAAGCTCTCATCCAATAGCATCTTGTTGCACAAAGCATCGAGCTTATGATATCAATAGGGTGGACAGCTTATAAACATTTCCATCTTGGTCACAAGATATTATATAAACTTGGCTTCCATTCAGTGAACGTTTTATTTCAAAGAGTTTCTTGCCTCTTTAGGTTTACAATAGGAAAACGTAACGCAATTCAGAACGACTAAGATAAATATAAACAGATGATAGTGACTCTTGTTGACTTACTTTCAGGGCACGGAGATGAGCAAGCCGATCGATAAGAAGTTCTACAAGGGCACCAATCCCAGCTGCCACGACTTCAACATAAGCTCGGCCACGCCCACGGGAGCGCCCCTTCTGGTGGGCTTCACCACAGGTCAAATCCAGCTG
 
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